Reviewed, UniProtKB/Swiss-Prot Q5KK25 (ATG26_CRYNE)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	ATG26
Ordered Locus Names:	CNC04500, CNBC2670

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	1585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.
Sequence caution	The sequence AAW42704.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1585	1585	Sterol 3-beta-glucosyltransferase		PRO_0000215608
Regions
	Domain	387 – 555	169	GRAM 1
	Domain	438 – 530	93	PH
	Domain	862 – 933	72	GRAM 2
	Compositional bias	3 – 32	30	Pro-rich
Natural variations
	Natural variant	83	1	D → N in strain: B-3501A.
	Natural variant	151	1	C → F in strain: B-3501A.
	Natural variant	789	1	M → I in strain: B-3501A.
	Natural variant	1487	1	H → L in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5KK25-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 05317838DF502414	FASTA	1,585	176,616
	10 20 30 40 50 60 MSPPISPTPP PLQPPFPPTA IARGPDRPDP PPQHQQAAES LVNAAAQHVA PTCPPTSDEL 70 80 90 100 110 120 PQMEDQATNS SNDSLIPSRQ APDQEETENA ITAGTPPDEM EPTKDAQTVR FSSSSPASYS 130 140 150 160 170 180 THEYPTEGIN EPRTSSRAPN TASSQMAESS CDFRSSRDIG SIRMGASALV SALNALPWEE 190 200 210 220 230 240 DDDSDDGEDD DEFIEPARGS SSTIYERKQR PQTPSTSHGF HPTHTLHFPF RQNAIARRAC 250 260 270 280 290 300 QPGTTELDYQ YATPETSSRR TSAAGSESSS EGEVPLPKGF VSHPNLIVPS GEGEAAAHPD 310 320 330 340 350 360 PKLISDRITK EQQIADVEEQ AEILRSAEEQ EMRLGKEFVP PKSRDSADLN VDAALREGGS 370 380 390 400 410 420 EREDVIEEQM QTNEAEKRLT RNEKLAERLM EVFGLEEREE VLEEMKCWLL RSVMLKGYMY 430 440 450 460 470 480 LTKRHICFFA NMPNENNLLV KSGPLHKKAS RSKLNTKFWV VLKNDVLSWY ESTSDPYFPK 490 500 510 520 530 540 GNISLQYCHS CDAVSGTRFK VRTSERNYTF TADTESSRDE WVKAIQKVMF KTQHEGETIK 550 560 570 580 590 600 LIIPLEAIVD VEKSPTLEFT ETIEVKCIDA EDQMSVDSYF FASFPDNDYA FSAIQKLVRE 610 620 630 640 650 660 RPSPPELPRI SSVTTIHANQ EPLDTSHATI KRHGTDSSAE KLGMASHRPF RKISSVLKPL 670 680 690 700 710 720 ILKSSDGEPL EEHSQGPHHN DEDASHLPHI EAISNRRRSE EESDNDYFDG YPPRQVGPPP 730 740 750 760 770 780 PSMNDDARNW RPSWIRKPAS KLFGSSPSGS FVSHPGRLPT DSSTTVTESG PSLRSRTGRT 790 800 810 820 830 840 KQASVTEVME PPIQYEEEVS EDEMSNKPSV VDSNSAETAR KRAARLSWTS ETSSGSQMVK 850 860 870 880 890 900 SKSDFSMLGS ESGHSESAET VRKFRTFFAL SDKEELIDHF PGYLYRVLPV SGRFFISTNY 910 920 930 940 950 960 FCFRSSQLLY KTKESFRLMI IPIRDLYGLK AQKAFRFGHS GLTVVIKGHE EIFIEFRSAS 970 980 990 1000 1010 1020 RRKACIALLE ERMEAVRLSG ENTIVDSHKI EARIMEDLDE STPVEPKSPW PVSPSPLFGS 1030 1040 1050 1060 1070 1080 TTSTSFLEFK PEPMKITCLT IGSRGDVQPY IALCKGLQAE GHITKIATHG EYKAWVEGHG 1090 1100 1110 1120 1130 1140 IAFESVGGDP AELMQMCVDN GMFTVSFLKE GLQKFRGWLD DLLNSSWEAC QGSDLLIESP 1150 1160 1170 1180 1190 1200 SAMSGIHVAE ALRIPYYRAF TMPWTRTRAY PHAFAVPEHG RGGPYNYMTY TMFDQVFWRA 1210 1220 1230 1240 1250 1260 ISGQVNRWRR NVLGLDATTF DKMEQHKVPF LYNFSPTVVP PPLDWTEWIH VTGYWFLDKA 1270 1280 1290 1300 1310 1320 DEKQGEKSWT PPQGLVDFID KAHGEEKKVV YIGFGSIVVS DPEEMTRCVV EAVVNSGVCA 1330 1340 1350 1360 1370 1380 ILSKGWSDRG SKKGEPKGDS EGADGVKYPP EIFAIDSIDH GWLFPRIDAA CHHGGAGTTG 1390 1400 1410 1420 1430 1440 ASLRAGIPTI IKPFFGDQAF WAERVESLNV GSSIRRLTSH QLASALIKAT TDEKQISKAR 1450 1460 1470 1480 1490 1500 VVGEMIRKEN GITRAIEAIY RDLEYAKSII KSLPSTDDRT PERISSHLHP LTTADLSFNR 1510 1520 1530 1540 1550 1560 VRSRSRSRSR SSQGRFSPRR HTVDDDGWSV VSGGSRSRSG SASAVTSPER RPLNIGSALG 1570 1580 SHVFKTALLP NTFGKWRNLE EGDDR

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017343 Genomic DNA. Translation: AAW42704.1. Sequence problems. AAEY01000013 Genomic DNA. Translation: EAL22129.1.
RefSeq	XP_570011.1. XP_776776.1.
UniGene	Fne.7173
3D structure databases
SMR	Q5KK25. Positions 408-529, 435-532, 1034-1459.
ModBase	Search...
Genome annotation databases
GeneID	3256569. 4934932.
GenomeReviews	Gene locus CNC04500 in contig AE017343_GR.
KEGG	cnb:CNBC2670. cne:CNC04500.
Phylogenomic databases
eggNOG	fuNOG07241.
OrthoDB	EOG9908S8.
PhylomeDB	Q5KK25.
Enzyme and pathway databases
BRENDA	2.4.1.173. 2772.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. PF00201. UDPGT. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_CRYNE

Accession

Primary (citable) accession number: Q5KK25
Secondary accession number(s): Q55W70

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	February 9, 2010
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)