Reviewed, UniProtKB/Swiss-Prot Q5KH67 (PPN1_CRYNE)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	PPN1
Ordered Locus Names:	CNE01080

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole may be required for activation By similarity.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Endopolyphosphatase		PRO_0000058545
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 678	655	Vacuolar Potential
Amino acid modifications
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Potential
	Glycosylation	447	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential
	Glycosylation	616	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5KH67-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 8B3C696309AC617D	FASTA	678	77,209
	10 20 30 40 50 60 MRSPLLASLF ALALSIASSE AAISSTEQVP LSGSVPQLDH SELQNVRINK KRPLKGRFLH 70 80 90 100 110 120 ITDIHPDPHY KTGSTFDSGC HRKPKKDGKS EGKKATENER GNEDLDDKEF DTLIKNEDLA 130 140 150 160 170 180 GKWGTAVSDC DCPMSLVNIT FDWLKEEWAN EVDFVVWTGD NARHDIDRRI PRTPKEIFDS 190 200 210 220 230 240 NRMIVDRMLD AFGRDMPIVP SIGNNDIYPH NVLAAGPSRI TEEFLLIWKH FIPSEAAHVF 250 260 270 280 290 300 ERGAYFSVEV IPDRLAVISL NTLFWYDANT LVDGCRDHSN DPGALEMDWL EVQLNNFRQR 310 320 330 340 350 360 GMQVWLTGHV PPHMNHYYDN CYLRYGDLAL RYQDTIVGHL FGHMNVDHFF FIDVDELEAT 370 380 390 400 410 420 SELTSTSSNT TLSDLPLLHG PRLPRPGPGK YTAMGRSGAR KLEEELRKDF GQMPGPGILK 430 440 450 460 470 480 LKDYAVMNVA PSVIPTYYPG IRIFSYNISD EEDSFDQGHS YQGADELLDD EDEGDELEEL 490 500 510 520 530 540 ELPSDNGFFG GLDERENEDI EILKGSGGHR HDVPKGDCSL PSNEDKPHCT FKRKPRHYSK 550 560 570 580 590 600 RSPSRTNRAL SPLGYTQFYL PSMMKQKKRP KWEVEYTTYK VKTLVPSSPE NTTQPLPVPL 610 620 630 640 650 660 HLLPRYDPSI FSKPKNKTEE KEVAKKTAKF YKAVKAVTPY RMKDLTIGSW VKLARMLVLE 670 KKRWKKFAEL MLVSTETD

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017345 Genomic DNA. Translation: AAW43547.1.
RefSeq	XP_570854.1.
UniGene	Fne.5186
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3257645.
GenomeReviews	Gene locus CNE01080 in contig AE017345_GR.
KEGG	cne:CNE01080.
Phylogenomic databases
HOGENOM	Q5KH67.
OMA	Q5KH67. KATENER.
Enzyme and pathway databases
BRENDA	3.6.1.10. 2772.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. IPR004843. M-pesterase. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF027093. EndopolyPtase_N1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPN1_CRYNE

Accession

Primary (citable) accession number: Q5KH67

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)