Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Alpha-amylase, putative

Gene

CNE03480

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Calcium 1UniRule annotation
Metal bindingi182 – 1821Calcium 1; via carbonyl oxygenUniRule annotation
Metal bindingi195 – 1951Calcium 1UniRule annotation
Active sitei226 – 2261NucleophileUniRule annotation
Metal bindingi226 – 2261Calcium 2UniRule annotation
Metal bindingi230 – 2301Calcium 1; via carbonyl oxygenUniRule annotation
Active sitei250 – 2501Proton donorUniRule annotation
Metal bindingi250 – 2501Calcium 2UniRule annotation
Sitei318 – 3181Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseImported, Hydrolase

Keywords - Ligandi

CalciumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-amylase, putativeImported (EC:3.2.1.1Imported)
Gene namesi
Ordered Locus Names:CNE03480Imported
OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)Imported
Taxonomic identifieri214684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000002149: Chromosome 5

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 58UniRule annotation
Disulfide bondi170 ↔ 184UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi214684.CNE03480.

Structurei

3D structure databases

ProteinModelPortaliQ5KGI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ5KGI6.
KOiK01176.
OMAiNQTQVED.
OrthoDBiEOG7RBZJ4.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013777. A-amylase_fun.
IPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5KGI6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTYQAALAL IPFISFLPAY ALDADAMRSR SVYQVVTDRF ARNSSSTGDC
60 70 80 90 100
NVADRTYCGG TWSALADKLD YIQGMGFDTV WISPVVENIG GTTGEGEAYH
110 120 130 140 150
GYWTLDPESL NSNFGSADDL KTLSSSLHAR GMYLQVDVVV NHVAATSSST
160 170 180 190 200
FQPSESYGPF STSEDYHPFC WITDYNNQTN VEQCWLGDDS VALADLNTES
210 220 230 240 250
DTVVSYWNQW ITDLVSNYTF DAIRIDTVKH VRQSFWPDFV NAAGVFNQGE
260 270 280 290 300
VLLADVPYVA GYQKNASINP FNYPVYYPLV RAFNGTGQSF SELINMVSYV
310 320 330 340 350
NSNFTDPTLL GTFLDNHDNA RFESFVTDPS LIKNAQAYPL VTDGIPYVYY
360 370 380 390 400
GSEAGFNGGN DPENREPMWT SNYDTSSDMY KFFTSLNAAR SAAGNASSTF
410 420 430 440 450
YTDKMTLSQP SESTLLVAKK PLMSVFSNSG SSASNTSVSV DSSSSGWAAS
460 470 480 490 500
TNVIDALTCE SFTTDDSGNL AVTIYQGEPR VLIEDSQKGS LCGGSSSNSS
510 520 530
STSNDSGAGM TTKVSGWVAL AGVFVAGLQV LL
Length:532
Mass (Da):57,639
Last modified:February 15, 2005 - v1
Checksum:i05971682AD484190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017345 Genomic DNA. Translation: AAW43607.1.
RefSeqiXP_570914.1. XM_570914.1.
UniGeneiFne.7153.

Genome annotation databases

EnsemblFungiiAAW43607; AAW43607; CNE03480.
GeneIDi3257780.
KEGGicne:CNE03480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017345 Genomic DNA. Translation: AAW43607.1.
RefSeqiXP_570914.1. XM_570914.1.
UniGeneiFne.7153.

3D structure databases

ProteinModelPortaliQ5KGI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214684.CNE03480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAAW43607; AAW43607; CNE03480.
GeneIDi3257780.
KEGGicne:CNE03480.

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiQ5KGI6.
KOiK01176.
OMAiNQTQVED.
OrthoDBiEOG7RBZJ4.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013777. A-amylase_fun.
IPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
    Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
    , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J., Koo H.L., Krzywinski M.I., Kwon-Chung J.K., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
    Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JEC21 / ATCC MYA-565Imported.

Entry informationi

Entry nameiQ5KGI6_CRYNJ
AccessioniPrimary (citable) accession number: Q5KGI6
Secondary accession number(s): Q55S33
Entry historyi
Integrated into UniProtKB/TrEMBL: February 15, 2005
Last sequence update: February 15, 2005
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.