Reviewed, UniProtKB/Swiss-Prot Q5KFE0 (ATM_CRYNE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase TEL1
    EC=2.7.11.1
Alternative name(s):
    DNA-damage checkpoint kinase TEL1
    Telomere length regulation protein 1
    ATM homolog

Gene names

Name:	TEL1
Ordered Locus Names:	CNF02070, CNBF2640

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	2967 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Associates with DNA double-strand breaks By similarity.
Subcellular location	Nucleus By similarity. Telomere By similarity. Note: Localizes to nuclear DNA repair foci with other DNA repair proteins in response to DNA double strand breaks By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. ATM subfamily. Contains 1 FAT domain. Contains 1 FATC domain. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Chromosomal protein Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chromatin regulator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2967	2967	Serine/threonine-protein kinase TEL1		PRO_0000227700
Regions
	Domain	1935 – 2523	589	FAT
	Domain	2651 – 2967	317	PI3K/PI4K
	Domain	2931 – 2967	37	FATC
Natural variations
	Natural variant	142	1	A → V in strain: B-3501A.
	Natural variant	171	1	L → H in strain: B-3501A.
	Natural variant	336	1	G → R in strain: B-3501A.
	Natural variant	395	1	V → I in strain: B-3501A.
	Natural variant	444	1	S → N in strain: B-3501A.
	Natural variant	472 – 478	7	NDENNND → DDDQSHN in strain: B-3501A.
	Natural variant	501	1	P → S in strain: B-3501A.
	Natural variant	786	1	A → V in strain: B-3501A.
	Natural variant	795	1	E → EE in strain: B-3501A.
	Natural variant	854	1	P → A in strain: B-3501A.
	Natural variant	915 – 916	2	HE → DD in strain: B-3501A.
	Natural variant	972	1	G → A in strain: B-3501A.
	Natural variant	1128	1	D → Q in strain: B-3501A.
	Natural variant	1275	1	T → A in strain: B-3501A.
	Natural variant	1342	1	A → V in strain: B-3501A.
	Natural variant	1380	1	E → K in strain: B-3501A.
	Natural variant	2162	1	V → A in strain: B-3501A.
	Natural variant	2165	1	K → R in strain: B-3501A.
	Natural variant	2254	1	Y → D in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5KFE0-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: FA723CFFAF25F542	FASTA	2,967	330,906
	10 20 30 40 50 60 MDSVSGLHAA LSLCASDSAK DRARAHALLP PIFANAQNLR VFQAAAATHG GAPWLALFHC 70 80 90 100 110 120 LFRAVALEKR AVLRGSSNAQ AAARLAHAIR IVRLVAEQAV HLIARKPLIA LVAHMRHQLV 130 140 150 160 170 180 LPPRIFAPAL LDYSKALSTL LAYPPHVESL DRHTWLALMS MCFAAILGDD LVSDDQMDDA 190 200 210 220 230 240 DMLGVAAELE RLDAENVPPH RPPVTLNTSS LVQIIPALLC STVSPIVPPT MKSGDTLTAG 250 260 270 280 290 300 QKVGLGIVLK IRRFFTMYPH VTIYHLHLLT ALNTVLSDME LNCRDLFLLG SVKIFPHLVT 310 320 330 340 350 360 LWAAPERDRD RRIPEQIAIA IHRILPHLAH SAELQGAQDV TENVERLMEM LAKESTMSRG 370 380 390 400 410 420 IEPLDLGTFP STATPSRQST PSKRRKLENP LASLVSAVGM GRPESRLIAL QTLVFVIDRH 430 440 450 460 470 480 WSKLAKDIQS DIRRTLVSLL TDDSETLQSW AYIALSTIIL SSYTAGANEN NNDENNNDGQ 490 500 510 520 530 540 SQDDWKQVWS FALRKCHLPG PSRSASHAAA VLLQFDKVDS PSTIRGIQTM LTTIAVQGPP 550 560 570 580 590 600 TAHDSVCALY SIALEAARSD IQLYSLNLEE QVLSWLEKTF AREKFERDKM DQRLDQATPG 610 620 630 640 650 660 DILRLFSAVS RIQYHVPLAE PVTKEFLPDS AVVSYALERA KTQPIRDFLL YHTCPTPPPP 670 680 690 700 710 720 PPPLDDSHTT PLLAASENHS TFLGGRPRRL SELLLSILNA TTAQWETKPV ISSGERPRRC 730 740 750 760 770 780 VDLVALGLAF QGLLQLDGYI PHAACINAAI RLLHLLKPSL TSSDLSIPSL DLVWRGLRCL 790 800 810 820 830 840 ADVPLAKEEE EEEEEDWPIL VKPDVQSGIR QDLLPPTMYD TPPQEEEAES SDPPKRFTQY 850 860 870 880 890 900 PPTFPSTLLP TPTPITPSSL STFQSQSNSA SLVHAIWRLP DVSAALQGLF SVCLQVITRS 910 920 930 940 950 960 SSSTSSGQPT QLSHHEDDDG EDDDDDFAVA SGETTSAPLP EKAAELRAST SLLRSAVAFR 970 980 990 1000 1010 1020 LQGVMLVSAG AGGGAQSKAY KDTQLVNSFL QADGLRAVEI GWAICEAMQK GWLRLGIDAV 1030 1040 1050 1060 1070 1080 DLVVASLGNM LNSYGYARDE RLLQLCLEFL KCSAPVWMGN DHSNDDDLKD EAMRLVCYIA 1090 1100 1110 1120 1130 1140 TKITAGSITS WRVRLAMLRF IEAFLHYDSA SKLWADCMVQ EEAEEEEDDV SMEDENHLFH 1150 1160 1170 1180 1190 1200 YIAQSLSDPD MRVRARAATT AASALYRPSI HPSEHPIIYD QVSNSQAGDP TFAEHYLSYV 1210 1220 1230 1240 1250 1260 LWKLNCCIAS AKQRQTVIFD LYEAAIGGTE YSDHLQAGLN AVARRLGLPS ITALYLPYAP 1270 1280 1290 1300 1310 1320 TTTISHDTSR SSAITFVLNT THRLFGLSNR SAFFTACLEH AGAYMLYCGK IGLFTSACDA 1330 1340 1350 1360 1370 1380 AGVLPEDLAL QLSVAAAAMA MALPLSNDKA TNVSMNKCKA EALALLSSFP GISGPDAAEE 1390 1400 1410 1420 1430 1440 FLHSYANGVA AHLWELMDLE SSVDEIVAWF DKTEKESAAG IAFAQMMAHD NAKTGRVKAI 1450 1460 1470 1480 1490 1500 NPAASFQSIH NVCRFLEKNY SSISLHAFTF AAILRLTSLI NNAFLVSEQR RYLRALAMLL 1510 1520 1530 1540 1550 1560 SVHQGTLQRP LIWEAFLTET ITLLLQPDIC RTVLSMVMWA FDWLESLSST PSKLVNIFCQ 1570 1580 1590 1600 1610 1620 LGEIRIELGG SGTPGSQPNQ MGDALEDWIV KMSPKWFKSQ ISQEAFERAV ALWPDSLRSR 1630 1640 1650 1660 1670 1680 LSVHASPLSL RDLDDLSQND AVHNAGQLCK HFLHLADADH GQDVVSVFVD STFWSLKDKI 1690 1700 1710 1720 1730 1740 SSVWDKEGIN AFQDLLYLCN GEVRAPSLNF YGQDTFSKAL NATTGEHKKT EAMNALYHAM 1750 1760 1770 1780 1790 1800 CKTMVQLLHD RRPQIRSAAY RCLQRMKPIL TGKDLKELPA DVSDVVPILV PIPIGSVRQS 1810 1820 1830 1840 1850 1860 QAMKLDGVIN NATWNKKAEH FATWSLELSR LLCKTASQHD KFFLSFEPLL STPLLPLHHF 1870 1880 1890 1900 1910 1920 LGHFVHAALV CSRSMSSERS KAISEHFETV LQNPFASIEA VRSIVNIVLQ LRRYEHPFTS 1930 1940 1950 1960 1970 1980 GMLGYNAWLS VNFVDLSKAA VKCGLYVSAL LFLELANDQG ESLDLAEPRV RQIMYDIYSN 1990 2000 2010 2020 2030 2040 VEDPDGFYGI HNKDIRDSLR RRLEHEGLSW QALGWAGAVY NVDGNDSRSA IPVLHHLHDI 2050 2060 2070 2080 2090 2100 GLSRLASVVA TETRTSGSVP LDDPFFADLS WRTGDWNLPI GRESSATSSG LLYSALSAVH 2110 2120 2130 2140 2150 2160 RSKSYESASK IVDKAVRAEM TRLGGLQKEM LTSIQSTVTN LLCLRELNRW LDPQLQQGMQ 2170 2180 2190 2200 2210 2220 EVIDKGTLRD LQDINDKFEF ASAERIIATR LSVFDSVKQR ESQDMIGDAL TPKMELVTKA 2230 2240 2250 2260 2270 2280 ESTCHIKLSR LALKSNNLQA AINSLTALQK LQTYVGVIDE AQDVFCEVLW KQGEHTLAIQ 2290 2300 2310 2320 2330 2340 LLEDLLLREK EKKSKGQRIP ALEGRLAHWA SEARLKAANE IFGMFSNVTK SIKRSTADVS 2350 2360 2370 2380 2390 2400 EHAEIFYQFA CFADKQYVSQ SSSADVKQLK EYSKLRASQA LRLSARQSRA RESDQKDSAV 2410 2420 2430 2440 2450 2460 REAERDEEKL KKFEMQQKQY LNAALQFYAE AVSMSDNFND CITRLVTLWL ENDENEESNV 2470 2480 2490 2500 2510 2520 TFSRAAHKVP SYKFIFLGPQ LAARLHRPES PTIFNSTLNG LMFRMSQDHP YHTLYHVIPL 2530 2540 2550 2560 2570 2580 LWEHKQPQST NSSMLGRKSA ADDIMRRLAS SASNRLAVGA AKSMKRFVAI AMEWTSFFEK 2590 2600 2610 2620 2630 2640 DKRLEYKLPS DSPLRKAPRD IPVATSTPSI DVTCQYKDIA TFDHFSEWYT RAGGLSRPKV 2650 2660 2670 2680 2690 2700 MTCFDSNGQK YTQLFKKDDG FRQDAVMEQI FVLVNDLLNR NRQTRSRKLR YRTYGVLALP 2710 2720 2730 2740 2750 2760 EATGVIEFVV GTKPLIKYLP PAHEKYHPKD ITSHDFLKAM QEVQSVKNND EKIIQVWTKL 2770 2780 2790 2800 2810 2820 KKRFRPVMRH LFTEKYRDPM AWFSMRLTYA RSLAVTSIVG WVLEIGDRHC SNILMDECTG 2830 2840 2850 2860 2870 2880 ELVHIDFGIA FGAGRILPIP ELVPFRLTDD LVDALGVTGV NGTFRQCSQL VLQTLIDSSD 2890 2900 2910 2920 2930 2940 VILTILEVFK QDPLHTWMVD DKMKKAQDGN HKMYPERGQE KADRIMRETR ENLSKELSVQ 2950 2960 YRVNQLIQEA RDVNNLATIF RGWHSWL

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017346 Genomic DNA. Translation: AAW44228.1. AAEY01000031 Genomic DNA. Translation: EAL20188.1.
RefSeq	XP_571535.1. XP_774835.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3258065. 4936816.
GenomeReviews	Gene locus CNF02070 in contig AE017346_GR.
KEGG	cnb:CNBF2640. cne:CNF02070.
Phylogenomic databases
HOGENOM	Q5KFE0.
Enzyme and pathway databases
BRENDA	2.7.11.1. 2772.
Family and domain databases
InterPro	IPR015519. Ataxia_Telang_Mut. IPR003152. FATC. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR003151. PIK-rel_kinase_FAT. IPR014009. PIK_FAT. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR11139:SF3. Ataxia_Telang_Mut. 1 hit.
Pfam	PF02259. FAT. 1 hit. PF02260. FATC. 1 hit. PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS51189. FAT. 1 hit. PS51190. FATC. 1 hit. PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATM_CRYNE

Accession

Primary (citable) accession number: Q5KFE0
Secondary accession number(s): Q55QS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)