Q5KEQ1 (CET1_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA-capping enzyme subunit beta
EC=3.1.3.33

Alternative name(s):
Polynucleotide 5'-triphosphatase
mRNA 5'-triphosphatase
Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	CNF04680

Organism

Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)

Taxonomic identifier

214684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	mRNA-capping enzyme subunit beta		PRO_0000210114

Sequences

	Sequence		Length	Mass (Da)
	Q5KEQ1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: B64BD4442048074C	FASTA	397	44,250
	10 20 30 40 50 60 MSTYIPIHDR PPHYANLPSP PSNSRQPRPP RYSSPDIDPD IPLARMSSAH ESDPSEQANA 70 80 90 100 110 120 TQSGDYVPRQ RVGSQGAEQE GQSRKRARTS PSHSGGNGSY VPRQRTEESH HSHHNGHSGH 130 140 150 160 170 180 AQSDQHGNGQ VYRPRHGQAP LTGSIFNLSP RNPFTSVVGD FIMNAAMGHS NVEIELKLGT 190 200 210 220 230 240 FMTPSMPGQQ PRRINMPTLS EMIIPHDYPN GPFVSTINHL HHRTLNELLN RAVESQSTHP 250 260 270 280 290 300 TGRLYFSRSK LADSFYDHSE HGHGKVRVSR DMDNGHVVQA VEKRRIADLN VYCPGMAYDF 310 320 330 340 350 360 RISVNTETPC EVPTGNAKSV RYKDRACYRH QVCRVDLTSV FSSNPRNADV PPSRSFELEI 370 380 390 EVLDVPALLA EGAAQSERFD EILQNVLDSA RMLVKNI

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JEC21 / ATCC MYA-565.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017346 Genomic DNA. Translation: AAW44125.1.
RefSeq	XP_571432.1. XM_571432.1.
3D structure databases
ProteinModelPortal	Q5KEQ1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3258470.
GenomeReviews	Gene locus CNF04680 in contig AE017346_GR.
KEGG	cne:CNF04680.
Phylogenomic databases
HOGENOM	HBG068117.
OMA	MSSELEM.
OrthoDB	EOG42Z80Z.
Family and domain databases
InterPro	IPR023577. CYTH-like_domain. IPR004206. mRNA_capping_enz_bsu_dom. [Graphical view]
Gene3D	G3DSA:3.20.100.10. mRNA_capping_enz_bsu. 1 hit.
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
SUPFAM	SSF55154. mRNA_capping_enz_bsu. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CET1_CRYNJ

Accession

Primary (citable) accession number: Q5KEQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 15, 2005
Last modified:	October 19, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program