Reviewed, UniProtKB/Swiss-Prot Q5K9H0 (BUD32_CRYNE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase BUD32
    EC=2.7.11.1

Gene names

Name:	BUD32
Ordered Locus Names:	CNK01970, CNBK1570

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAW46242.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosomal protein Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Serine/threonine-protein kinase BUD32		PRO_0000278912
Regions
	Domain	8 – 289	282	Protein kinase
	Nucleotide binding	14 – 22	9	ATP By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5K9H0-1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: 5163B24144B287DF	FASTA	289	31,670
	10 20 30 40 50 60 MAASTPLLSR GTLIKQGAEA KVYALPSLFP EPTTYHPGSS SSFSAASPTP VILKHRFTKT 70 80 90 100 110 120 YRHPTLDASL TSQRLTFEAR ALARAAKAGV TVPKVVWVDE KAGVIGMERI EGWSVREILG 130 140 150 160 170 180 GGAEGEVEVI EEQEIEEDVE NKAEDSAVRE EPEGPESEGL KALKNLGVTQ EHLMRSIGAA 190 200 210 220 230 240 LARLHKTMII HGDLTTSNMM VRLTPGGSGP YEIVLIDFGL SSQAQFPENY AVDLYVLERA 250 260 270 280 FASTHPRSEK LYAGVLETYA EGLGEKKWKP IQIKLKDVRR RGRKRDMTG

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017351 Genomic DNA. Translation: AAW46242.1. AAEY01000052 Genomic DNA. Translation: EAL18136.1.
RefSeq	XP_567759.1. XP_772783.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3254467. 4938853.
GenomeReviews	Gene locus CNK01970 in contig AE017351_GR.
KEGG	cnb:CNBK1570. cne:CNK01970.
Phylogenomic databases
HOGENOM	Q5K9H0.
Enzyme and pathway databases
BRENDA	2.7.11.1. 2772.
Family and domain databases
InterPro	IPR002575. APH_trans. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF01636. APH. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_CRYNE

Accession

Primary (citable) accession number: Q5K9H0
Secondary accession number(s): Q55K29

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	June 16, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)