Reviewed, UniProtKB/Swiss-Prot Q5K8R1 (ALG3_CRYNE)

Names and origin

Protein names

Recommended name:
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    EC=2.4.1.130
Alternative name(s):
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Asparagine-linked glycosylation protein 6

Gene names

Name:	ALG3
Ordered Locus Names:	CNL04800, CNBI2040

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Transfers an alpha-D-mannosyl residue from dolichyl-phosphate D-mannose into membrane lipid-linked oligosaccharide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase		PRO_0000350925
Regions
	Topological domain	1 – 34	34	Lumenal Potential
	Transmembrane	35 – 55	21	Potential
	Topological domain	56 – 83	28	Cytoplasmic Potential
	Transmembrane	84 – 104	21	Potential
	Topological domain	105 – 118	14	Lumenal Potential
	Transmembrane	119 – 139	21	Potential
	Topological domain	140 – 170	31	Cytoplasmic Potential
	Transmembrane	171 – 191	21	Potential
	Topological domain	192 – 204	13	Lumenal Potential
	Transmembrane	205 – 225	21	Potential
	Topological domain	226 – 232	7	Cytoplasmic Potential
	Transmembrane	233 – 253	21	Potential
	Topological domain	254 – 296	43	Lumenal Potential
	Transmembrane	297 – 317	21	Potential
	Topological domain	318 – 343	26	Cytoplasmic Potential
	Transmembrane	344 – 364	21	Potential
	Topological domain	365 – 379	15	Lumenal Potential
	Transmembrane	380 – 400	21	Potential
	Topological domain	401 – 411	11	Cytoplasmic Potential
	Transmembrane	412 – 432	21	Potential
	Topological domain	433 – 447	15	Lumenal Potential
Natural variations
	Natural variant	7	1	F → S in strain: B-3501A. Ref.1
	Natural variant	8	1	T → S in strain: B-3501A. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5K8R1-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 5D466EAF24A77013	FASTA	447	50,713
	10 20 30 40 50 60 MSRQSLFTPA LGQRKGLISH TVDLVRALLF DRRYFWHTAF LLFLGEVALS LLVIWKIPYT 70 80 90 100 110 120 KIDWPAYMQQ VDMFLAGERD YSKIEGETGP LVYPALHLYI YTAFHRLLPS IENVRPAQFV 130 140 150 160 170 180 FLGFYLATYL AISTIYYLAG RPSNGGHHFP QVLLIPLTLS KRAHSIFLLR LFNDPIAMLI 190 200 210 220 230 240 FYLSVIAFQI GGRKGWRLGC VLFSLALGVK MNILNFLPGL LVLLFQYRGI VGTVEGLSII 250 260 270 280 290 300 GLIQFLLPAP FFFSKSNPYL IRAYFTSAFD FSRQFLYEWT VNWRFISEET FLSRERAVTL 310 320 330 340 350 360 LAGHLTVLGL FAAFKWSPVP GGTLRVLRKG FSDPLNQALE VSQVPAYHIP LVLFSANLIG 370 380 390 400 410 420 MLFARSLHYQ FHSWYFHQLP FLLYSGAGWG NMLTSVLIWV TVQYAWETAP STISTSAALL 430 440 AGHGAMVFGL FFHGMKRPSS SQKSKAK

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS SER-7 AND SER-8. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017352 Genomic DNA. Translation: AAW46504.1. AAEY01000044 Genomic DNA. Translation: EAL18943.1.
RefSeq	XP_568021.1. XP_773590.1.
UniGene	Fne.3496
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5K8R1.
Genome annotation databases
GeneID	3254774. 4938164.
GenomeReviews	Gene locus CNL04800 in contig AE017352_GR.
KEGG	cnb:CNBI2040. cne:CNL04800.
Phylogenomic databases
eggNOG	fuNOG07998.
HOGENOM	HBG525447.
OMA	LRLFNDC.
OrthoDB	EOG9T4FBS.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_CRYNE

Accession

Primary (citable) accession number: Q5K8R1
Secondary accession number(s): Q55M08

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)