Reviewed, UniProtKB/Swiss-Prot Q5K8D3 (ATG1_CRYNE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATG1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1

Gene names

Name:	ATG1
Ordered Locus Names:	CNL06100, CNBI0730

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine protein kinase probably involved in the cytoplasm to vacuole transport (Cvt) and in autophagy, where it may be required for the formation of autophagosomes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAW46622.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 988	988	Serine/threonine-protein kinase ATG1		PRO_0000085644
Regions
	Domain	27 – 346	320	Protein kinase
	Nucleotide binding	33 – 41	9	ATP By similarity
Sites
	Active site	183	1	Proton acceptor By similarity
	Binding site	56	1	ATP By similarity
Natural variations
	Natural variant	140	1	D → N in strain: B-3501A.
	Natural variant	517	1	A → T in strain: B-3501A.
	Natural variant	617	1	A → T in strain: B-3501A.
	Natural variant	625	1	P → A in strain: B-3501A.
	Natural variant	962	1	D → E in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5K8D3-1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: CDF0107160D35036	FASTA	988	107,248
	10 20 30 40 50 60 MPDSNAQGSR EKESGHHRSH KERIGNYVVG AEIGRGSFAT VYKGYRSKTR VPIAIKAVSR 70 80 90 100 110 120 QKLTSKLLEN LESEINILKV INHRNIVALT DCFKNDTHIY LVMEYCSGSD LSVYIKQRGN 130 140 150 160 170 180 IPTLDFVPKA GSSMALLPTD DEGKIYWPHP PTGGLDERVT RSFLGQLAQA IKFLRAQDLM 190 200 210 220 230 240 HRDIKPQNLL LQPATETEVA EGHPYGIPVL KVADFGFARI LPAAAMAETL CGSPLYMAPE 250 260 270 280 290 300 ILRYEKYDAK ADLWSVGAVL FEMSVGRPPF RANNHVELLR RIEKSNDNIV FPDEKERDSK 310 320 330 340 350 360 SSDETSIPVP SDIKALIRAL LKRKPNDRMG FDDFFNCGVW DGHMAESTEE ESLSLDVSTD 370 380 390 400 410 420 SSAGLGESDR IRQMVASTEQ SKDRLIPTRA PQPLAADAAL NPQPAVPISR DTSEGRSRLS 430 440 450 460 470 480 TPPSRPTPTR RSTPKYYVGD ATPSEPTAII PPSPSSAPTP TSAPDPGLTT QQSPTSRANP 490 500 510 520 530 540 RPIITAASSA QRRMSGKGDG REASSVEEAA PITPPFAGPS PTMARPSRGI NEGSPLAAAP 550 560 570 580 590 600 PITMGPDGRL ERSSALEGST SGVGTDYVVV EKQTVEINAL ADELDQASKR PMIRRSSRGS 610 620 630 640 650 660 VVSRPVSSFK PISPNIAKND PTLGPISYSP PFALSSTPPF AMQVPRHASG GNSFPRNPSI 670 680 690 700 710 720 PSSLNAFPPT TISASPSYGQ DAAARFGVSP GSLQTGALAR ALTNTAIRLI GTSANTAATA 730 740 750 760 770 780 IARATAKRRP NIVRVSDMDA AEDDLLCTVE DLARKAFVLF ELADERLLAQ TQLAQTARTS 790 800 810 820 830 840 STPTGLTGTT PPFSMQAAAA AQAGSRRKSS SSSMNSEVWI LRQQEAAAND AVVLYMKSLA 850 860 870 880 890 900 FIVKAMDKVK RYWKNRTDVY DGYVASQELN EMGQWLRARF NETFEKAEWA KTQAGDTLLF 910 920 930 940 950 960 PDWLVHDKAR DTSRQAAVAE LQGDLTTAEQ GYETSLWLLQ ALLDESVYEN GSIPDEDKVA 970 980 YDRLMVPIKT RLDALRKKLA ESSGMTAR

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017352 Genomic DNA. Translation: AAW46622.1. Sequence problems. AAEY01000044 Genomic DNA. Translation: EAL18812.1.
RefSeq	XP_568139.1. XP_773459.1.
UniGene	Fne.283
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3255019. 4938026.
GenomeReviews	Gene locus CNL06100 in contig AE017352_GR. Gene locus CNBI0730 in contig CM000048_GR.
KEGG	cnb:CNBI0730. cne:CNL06100.
Phylogenomic databases
HOGENOM	Q5K8D3.
Enzyme and pathway databases
BRENDA	2.7.11.1. 2772.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_CRYNE

Accession

Primary (citable) accession number: Q5K8D3
Secondary accession number(s): Q55MD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 19, 2005
Last modified:	September 22, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)