Reviewed, UniProtKB/Swiss-Prot Q5K7E9 (NMT_CRYNE)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Ordered Locus Names:

CNN00080, CNBN0070

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.
Sequence caution	The sequence AAW47000.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAL17377.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064238
Regions
	Region	45 – 48	4	Myristoyl CoA-binding By similarity
	Region	179 – 215	37	Myristoyl CoA-binding By similarity
Sites
	Active site	493	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5K7E9-1 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: CC82641C5B7CFB02	FASTA	493	54,964
	10 20 30 40 50 60 MDSSDSKAAT DEEIRRALKA ADLMKILDGK IALGNKSGTK NLGEHKFWKT QPVPQITGSG 70 80 90 100 110 120 APAPIEEGPI DDPKTPADVR QEPGVLPAGF EWSTIDINDE EQSKEVYVLL CENYVEDDDA 130 140 150 160 170 180 MFRFNYSREF LLWALTAPGY LPDWHIGVRV QKTKKLVAFI SGIKIDIRVR AKTFPAAEIN 190 200 210 220 230 240 FLCVHKKLRS KRLAPVLIKE VTRRVNLTNI WQAIYTAGVI LPTPIGTCRY FHRNLNPPKL 250 260 270 280 290 300 VDIGFSPLPR GSTIARLVQQ YSVPSHPRIP GFREMKKEDV PQVGALLRRY LDRFDVAQAF 310 320 330 340 350 360 RDDDEVEHWL LSGQGKEVGG RRVEQVVWAY VVEDPTTHRI TDLISFYALP STIMKHPKHN 370 380 390 400 410 420 LLNAAYMFYY ATDVVFPPSS SSANSDVDVD ANAGESSVAA VGTGGEDAKT KKKLETRLNA 430 440 450 460 470 480 LTADILIIAK QAGFDVFNAL TLLDNNMFLQ EQKFGPGDGY LNYYLYNWNC APIDGGHHST 490 TAKQGSKIGV VML

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017356 Genomic DNA. Translation: AAW47000.1. Sequence problems. AAEY01000065 Genomic DNA. Translation: EAL17377.1. Sequence problems.
RefSeq	XP_568517.1. XP_772024.1.
UniGene	Fne.2994
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5K7E9.
Genome annotation databases
GeneID	3255415. 4939624.
GenomeReviews	Gene locus CNN00080 in contig AE017356_GR. Gene locus CNBN0070 in contig CM000053_GR.
KEGG	cnb:CNBN0070. cne:CNN00080.
Phylogenomic databases
HOGENOM	Q5K7E9.
Enzyme and pathway databases
BRENDA	2.3.1.97. 2772.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_CRYNE

Accession

Primary (citable) accession number: Q5K7E9
Secondary accession number(s): Q55HW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	January 10, 2006
Last modified:	September 22, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)