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Q5K2N1 (DET2_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 5-alpha-reductase DET2

Short name=LeDET2
EC=1.3.1.22
Gene names
Name:DET2
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Can use progesterone, testosterone, androstenedione and campestenone as substrate. Ref.1

Catalytic activity

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.1

Enzyme regulation

Repressed by steroid (4-MA, VG106, PD91, PD17, Finasteride) and non-steroid (AS601811, AFA27, AFA76, AFA131, AFA192) inhibitors; steroid inhibitors are generally more efficient. Ref.1

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Mostly expressed in leaves and hypocotyls and, to a lower extent, in stems, cotyledons, roots, seeds and callus. Ref.1

Sequence similarities

Belongs to the steroid 5-alpha reductase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.75 µM for progesterone (expressed in COS-7 cells, Ref.1) Ref.1

KM=26 µM for androstenedione (expressed in COS-7 cells, Ref.1)

KM=40 µM for testosterone (expressed in COS-7 cells, Ref.1)

Vmax=0.34 nmol/min/mg enzyme with progesterone as substrate (expressed in COS-7 cells, Ref.1)

Vmax=0.32 nmol/min/mg enzyme with androstenedione as substrate (expressed in COS-7 cells, Ref.1)

Vmax=0.21 nmol/min/mg enzyme with testosterone as substrate (expressed in COS-7 cells, Ref.1)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Steroid 5-alpha-reductase DET2
PRO_0000418515

Regions

Transmembrane11 – 3121Helical; Potential
Transmembrane47 – 6721Helical; Potential
Transmembrane78 – 9720Helical; Potential
Transmembrane110 – 13021Helical; Potential
Transmembrane151 – 17121Helical; Potential
Transmembrane200 – 22021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q5K2N1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 6D3DA1537BCC1CB7

FASTA25729,802
        10         20         30         40         50         60 
MFSSDENLFN FIVFFILVMA FPTFILCQFF TSPYGKHYTS ADSGTTISPP IAWAFMESPT 

        70         80         90        100        110        120 
LWLTIIVFRL GKNYTNPLAF LLISPYLFHY TNRTIIYPLR LRSRNTKNNF PLNIAVTAFI 

       130        140        150        160        170        180 
FNLLNAYIQS RWVSHYANYQ EDDWFWVRFG IGLVIFGSGM LLNIWADGVL LGLKSQGGGY 

       190        200        210        220        230        240 
KIPRGGLFDY VSSPNYLGEI MEWLGWALMT WSWAGLAFFV YTCANLVPRA VSNHKWYLQK 

       250 
FGEDYPKNRK AVFPFLY 

« Hide

References

[1]"5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: cv. Saint Pierre.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ786362 mRNA. Translation: CAH05260.1.
RefSeqNP_001234040.1. NM_001247111.1.
UniGeneLes.5994.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc10g086500.1.1; Solyc10g086500.1.1; Solyc10g086500.1.
GeneID543801.
KEGGsly:543801.

Phylogenomic databases

KOK09591.

Enzyme and pathway databases

BRENDA1.3.1.30. 279253.
UniPathwayUPA00381.

Family and domain databases

InterProIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDET2_SOLLC
AccessionPrimary (citable) accession number: Q5K2N1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways