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Q5K2N1

- DET2_SOLLC

UniProt

Q5K2N1 - DET2_SOLLC

Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Can use progesterone, testosterone, androstenedione and campestenone as substrate.1 Publication

    Catalytic activityi

    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

    Enzyme regulationi

    Repressed by steroid (4-MA, VG106, PD91, PD17, Finasteride) and non-steroid (AS601811, AFA27, AFA76, AFA131, AFA192) inhibitors; steroid inhibitors are generally more efficient.1 Publication

    Kineticsi

    1. KM=0.75 µM for progesterone (expressed in COS-7 cells, PubMed:15993049)1 Publication
    2. KM=26 µM for androstenedione (expressed in COS-7 cells, PubMed:15993049)1 Publication
    3. KM=40 µM for testosterone (expressed in COS-7 cells, PubMed:15993049)1 Publication

    Vmax=0.34 nmol/min/mg enzyme with progesterone as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

    Vmax=0.32 nmol/min/mg enzyme with androstenedione as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

    Vmax=0.21 nmol/min/mg enzyme with testosterone as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: InterPro
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. progesterone 5-alpha-reductase activity Source: UniProtKB

    GO - Biological processi

    1. brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
    2. oxidation-reduction process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.3.1.30. 279253.
    UniPathwayiUPA00381.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
    Short name:
    LeDET2
    Gene namesi
    Name:DET2
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Chromosome 10

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257Steroid 5-alpha-reductase DET2PRO_0000418515Add
    BLAST

    Expressioni

    Tissue specificityi

    Mostly expressed in leaves and hypocotyls and, to a lower extent, in stems, cotyledons, roots, seeds and callus.1 Publication

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei47 – 6721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei78 – 9720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the steroid 5-alpha reductase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK09591.
    OMAiVSAANYF.

    Family and domain databases

    InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5K2N1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSSDENLFN FIVFFILVMA FPTFILCQFF TSPYGKHYTS ADSGTTISPP    50
    IAWAFMESPT LWLTIIVFRL GKNYTNPLAF LLISPYLFHY TNRTIIYPLR 100
    LRSRNTKNNF PLNIAVTAFI FNLLNAYIQS RWVSHYANYQ EDDWFWVRFG 150
    IGLVIFGSGM LLNIWADGVL LGLKSQGGGY KIPRGGLFDY VSSPNYLGEI 200
    MEWLGWALMT WSWAGLAFFV YTCANLVPRA VSNHKWYLQK FGEDYPKNRK 250
    AVFPFLY 257
    Length:257
    Mass (Da):29,802
    Last modified:February 15, 2005 - v1
    Checksum:i6D3DA1537BCC1CB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ786362 mRNA. Translation: CAH05260.1.
    RefSeqiNP_001234040.1. NM_001247111.1.
    UniGeneiLes.5994.

    Genome annotation databases

    EnsemblPlantsiSolyc10g086500.1.1; Solyc10g086500.1.1; Solyc10g086500.1.
    GeneIDi543801.
    KEGGisly:543801.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ786362 mRNA. Translation: CAH05260.1 .
    RefSeqi NP_001234040.1. NM_001247111.1.
    UniGenei Les.5994.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Solyc10g086500.1.1 ; Solyc10g086500.1.1 ; Solyc10g086500.1 .
    GeneIDi 543801.
    KEGGi sly:543801.

    Phylogenomic databases

    KOi K09591.
    OMAi VSAANYF.

    Enzyme and pathway databases

    UniPathwayi UPA00381 .
    BRENDAi 1.3.1.30. 279253.

    Family and domain databases

    InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
      Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
      J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Saint Pierre.
      Tissue: Leaf.

    Entry informationi

    Entry nameiDET2_SOLLC
    AccessioniPrimary (citable) accession number: Q5K2N1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3