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Q5K2N1

- DET2_SOLLC

UniProt

Q5K2N1 - DET2_SOLLC

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Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Can use progesterone, testosterone, androstenedione and campestenone as substrate.1 Publication

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

Enzyme regulationi

Repressed by steroid (4-MA, VG106, PD91, PD17, Finasteride) and non-steroid (AS601811, AFA27, AFA76, AFA131, AFA192) inhibitors; steroid inhibitors are generally more efficient.1 Publication

Kineticsi

  1. KM=0.75 µM for progesterone (expressed in COS-7 cells, PubMed:15993049)1 Publication
  2. KM=26 µM for androstenedione (expressed in COS-7 cells, PubMed:15993049)1 Publication
  3. KM=40 µM for testosterone (expressed in COS-7 cells, PubMed:15993049)1 Publication

Vmax=0.34 nmol/min/mg enzyme with progesterone as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

Vmax=0.32 nmol/min/mg enzyme with androstenedione as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

Vmax=0.21 nmol/min/mg enzyme with testosterone as substrate (expressed in COS-7 cells, PubMed:15993049)1 Publication

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: EnsemblPlants/Gramene
  2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  3. progesterone 5-alpha-reductase activity Source: UniProtKB
  4. sterol 5-alpha reductase activity Source: EnsemblPlants/Gramene

GO - Biological processi

  1. brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
  2. oxidation-reduction process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.30. 279253.
UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
Short name:
LeDET2
Gene namesi
Name:DET2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Chromosome 10

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence AnalysisAdd
BLAST
Transmembranei47 – 6721HelicalSequence AnalysisAdd
BLAST
Transmembranei78 – 9720HelicalSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence AnalysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: EnsemblPlants/Gramene
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Steroid 5-alpha-reductase DET2PRO_0000418515Add
BLAST

Expressioni

Tissue specificityi

Mostly expressed in leaves and hypocotyls and, to a lower extent, in stems, cotyledons, roots, seeds and callus.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ5K2N1.
KOiK09591.
OMAiVSAANYF.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5K2N1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSSDENLFN FIVFFILVMA FPTFILCQFF TSPYGKHYTS ADSGTTISPP
60 70 80 90 100
IAWAFMESPT LWLTIIVFRL GKNYTNPLAF LLISPYLFHY TNRTIIYPLR
110 120 130 140 150
LRSRNTKNNF PLNIAVTAFI FNLLNAYIQS RWVSHYANYQ EDDWFWVRFG
160 170 180 190 200
IGLVIFGSGM LLNIWADGVL LGLKSQGGGY KIPRGGLFDY VSSPNYLGEI
210 220 230 240 250
MEWLGWALMT WSWAGLAFFV YTCANLVPRA VSNHKWYLQK FGEDYPKNRK

AVFPFLY
Length:257
Mass (Da):29,802
Last modified:February 15, 2005 - v1
Checksum:i6D3DA1537BCC1CB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ786362 mRNA. Translation: CAH05260.1.
RefSeqiNP_001234040.1. NM_001247111.1.
UniGeneiLes.5994.

Genome annotation databases

EnsemblPlantsiSolyc10g086500.1.1; Solyc10g086500.1.1; Solyc10g086500.1.
GeneIDi543801.
KEGGisly:543801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ786362 mRNA. Translation: CAH05260.1 .
RefSeqi NP_001234040.1. NM_001247111.1.
UniGenei Les.5994.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi Solyc10g086500.1.1 ; Solyc10g086500.1.1 ; Solyc10g086500.1 .
GeneIDi 543801.
KEGGi sly:543801.

Phylogenomic databases

InParanoidi Q5K2N1.
KOi K09591.
OMAi VSAANYF.

Enzyme and pathway databases

UniPathwayi UPA00381 .
BRENDAi 1.3.1.30. 279253.

Family and domain databases

InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes."
    Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A., Serio M., Racchi M.L., Danza G.
    J. Steroid Biochem. Mol. Biol. 96:287-299(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Saint Pierre.
    Tissue: Leaf.

Entry informationi

Entry nameiDET2_SOLLC
AccessioniPrimary (citable) accession number: Q5K2N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3