Q5K2K3 (L_ISFV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Isfahan virus (ISFV)

Taxonomic identifier

290008 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Gerbillinae (gerbils) [TaxID: 10045]
Phlebotomus papatasi (Sandfly) [TaxID: 29031]

Protein attributes

Sequence length	2093 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2093	2093	RNA-directed RNA polymerase L		PRO_0000287260
Regions
	Domain	587 – 773	187	RdRp catalytic
	Nucleotide binding	1656 – 1665	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5K2K3 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: B65373A9AB43E2C9	FASTA	2,093	237,644
	10 20 30 40 50 60 MDEYSEEKWG DSDEESFGTG KYSDESRIRG LNSVDYNLNS PLIQDDLYYL MERVRGRPVP 70 80 90 100 110 120 PIWKAKNWTE TIHLVQESRL DYLPTQKLHS WYAEWLMEES HDSSQGLAFL KEVDKDSLET 130 140 150 160 170 180 YEVVMSFLRG WCGGAPAYKK KEGRHIAKIG SLCQKFLDLH RVILIMNAST QMELSNLAET 190 200 210 220 230 240 FQASSVSKKI ITTPSMGKME MSGQFALAYQ QKVILDRNFL LMMKDVVIGR MQTLLSMVSR 250 260 270 280 290 300 TDDKFSDGDI SYLIKIYQLG DKIIQSLGND GYELIKTIEP MCNLRLSDLA REYRPLIPEF 310 320 330 340 350 360 PHFRQHIEGT VSELRKKTAL IVDMFKMIDR TPGVDITLVI YGSFRHWGHP FIDYFAGLTK 370 380 390 400 410 420 LNSQVTMGKQ IDDEYVACLA SDLARIVLTK EFNEKKRWSV NYNLVPQDHP FHEHIRDNTW 430 440 450 460 470 480 PTPAVIQDFG DKWHELPLTQ CFEIPDLIDP SIIYSDKSHS MNRQDVLNHV KRKPDQPIPS 490 500 510 520 530 540 RKVLQTMIDT PATNWLEFLE EIDKNGLSDD DLVIGLKGKE RELKIAGRFF SLMSWKLREY 550 560 570 580 590 600 FVITEYLIKT HFVPLFHGLT MADDMTAVIK KMLESSSGQG LKDYSAVCIA NHIDYEKWNN 610 620 630 640 650 660 HQRKRSNEPI FKVMGQFLGF PNLISRTHEF FEKSLIYYNG RPDLMKVQDG RLVNTTKQLV 670 680 690 700 710 720 CWEGQAGGLE GLRQKGWSIL NLLVIQRESK IRNTAVKVLA QGDNQVICTQ YKTKQHRNET 730 740 750 760 770 780 ELRSALTQMK LNNDAVMKAI ESGTNKLGLL INQDETMQSA DYLNYGKVPI FRGVIRGLET 790 800 810 820 830 840 KRWSRVTCVT NDQLPTCANL MSSVSTNALT VAHFDVTPLN AMIQYNYFGN FSRLLLNMHD 850 860 870 880 890 900 PAVRCSLFQL SQKHKIDLFS FEFKVGVLYL DPSIGGVCGT ALSRFLIRGF PDPVTESISF 910 920 930 940 950 960 WKVIYNNTQD NRLKKLCTAF GNPKIAQFRY SHIEKLLEDP TSLNISMGMS AANLLKSEIK 970 980 990 1000 1010 1020 KNLLRKRRTI GNSIVRDAVT YIHSEDEKIR SYLWSINPLF PRFLSEFKSG TFMGVASSVV 1030 1040 1050 1060 1070 1080 SLFQNSRTIR NVFKDYMSSA IDELITKSEV NSLEHLCKYK GVRNFDQVWK CSASQADYLR 1090 1100 1110 1120 1130 1140 RLSWGRKVLG TTIPHPLEML GAGTIKNNSS TCCEHSGQDY ISVFCPKGIS NVLIERGPMA 1150 1160 1170 1180 1190 1200 AYLGSKTSES TSILQPWEKE SKIPIIKRAT RLRDAIHWFV EPSSNLAKSI LQNITALTGE 1210 1220 1230 1240 1250 1260 EWGSSLEGFK RTGSALHRFT TSRMSHGGFC AQSPAALTRM MATTDTMSDY AKDNYDFMFQ 1270 1280 1290 1300 1310 1320 ACLLFSQITT SVLLLETTIS NTVHFHTRCI NCVRKIEEPW LESPSVLQSK DVSNVLASWR 1330 1340 1350 1360 1370 1380 NGGGSWGEQL HQLKPLKGDW EILTPAEKSY HVGRTLGFLF GDLTGQSSIR ADDSSLFPLS 1390 1400 1410 1420 1430 1440 IQKRLRGRGF LRGVLDGLVR ASACQVIHRR SLTQLKRPAN AVYGGLIFLI DKISASSTFI 1450 1460 1470 1480 1490 1500 NLCRDGPIRE ELSSIPHKIP TSYPTSNADL GLHIRNYFKF QCKSVELGKY QSDLEDLWLF 1510 1520 1530 1540 1550 1560 SDLLSSGFAG PYALSSKVLK SLYKPSLSRR DRNNIRKLGA LSRLLRSHEN WSELHKEFLT 1570 1580 1590 1600 1610 1620 SQLLLCQEEV RHACKFGIPK NVSAKSSMVW GKEAVSYVLD IPVEFTSQKQ TKHLNACPRI 1630 1640 1650 1660 1670 1680 QDPTISGLRL GQLPTGAHYK IRTILNAYNI KCRDVLCGGD GSGGMTAACL RYYSNSRAIF 1690 1700 1710 1720 1730 1740 NSILEFDGSS MKGSSPDPPS ALETVDQGMV RCVNATTCWE NPSDLSQERT WDYFLHLKKS 1750 1760 1770 1780 1790 1800 FNMKIDLIIL DMEVRDFQIS KLIEGNLRLK ISKLLEKNGT LIYKTYGTII CSETSNVLTT 1810 1820 1830 1840 1850 1860 LGPLFHSVYI VQTGYSSSFT SEVYVLFSKQ KSFVDSPYVD WGSLQYNWEK LACFRNPRQE 1870 1880 1890 1900 1910 1920 FKRALRIRSS RSLMGIPSSF LPDPLVNLET LLQISGVPSG VSHQLVTDVK SSGASGLSSA 1930 1940 1950 1960 1970 1980 IGLLGLISHF TLDVTKLYVQ EYRPPSDNRL IKMASAITGI SYWISIAYHD QQLNQALTSV 1990 2000 2010 2020 2030 2040 IKKSFPIRWG LINHRLHWSV SDRFHRSKDV RLSDCLAGIG NWIRGMELMK LPAGMFSHKE 2050 2060 2070 2080 2090 VNMILSKYIR GLNYHTISSR TGILEILKSQ FSIIDRSLMT ITTDNIQSSD WTD

References

[1]	"Complete genome sequences of Chandipura and Isfahan vesiculoviruses." Marriott A.C. Arch. Virol. 150:671-680(2005) [PubMed: 15614433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ810084 Genomic RNA. Translation: CAH17548.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_ISFV

Accession

Primary (citable) accession number: Q5K2K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	February 15, 2005
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program