Q5K2C4 (TPS1_APHAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase 1
UDP-glucose-glucosephosphate glucosyltransferase 1

Gene names

Name:

tps-1

Organism

Aphelenchus avenae (Mycophagous nematode worm)

Taxonomic identifier

70226 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Tylenchida › Aphelenchina › Aphelenchoidea › Aphelenchidae › Aphelenchus

Protein attributes

Sequence length	1155 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the production of trehalose from glucose-6-phosphate and UDP-glucose in a 2 step process By similarity.
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the gob-1 trehalose phosphatase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q5K2C4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: Q5K2C4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1155	1155	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1		PRO_0000385173
Natural variations
	Alternative sequence	1 – 175	175	Missing in isoform b.		VSP_038109

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 5D15061B15C32E47	FASTA	1,155	130,891
	10 20 30 40 50 60 MSHGTKYRDA LLFSLTLYDV NTGKSRLKEL YAAVPGIRKS LLGVHAKRFG EQYHHLQRRR 70 80 90 100 110 120 SVSSRGGSLR GSMDSLNDSG QNGAEDVIGV EDEEEAQKFR GKRTSISLDP AAAGEVMFTI 130 140 150 160 170 180 EDGACFPSGG LANTHFQQRV INVSNAPPVS LKREKSGEWE IKQGSGGLVS CVDPIMSVNQ 190 200 210 220 230 240 ENMWLANLGM NIDKKKMLRS TELLNVTDDS APLAPATNTL GLPLMRQALA DVLFHVIADD 250 260 270 280 290 300 DIKEQNEDEQ SRNVRWGHSS VEAGGVAPSQ PWEEMSLLGV LNQYNRSNYK LNPVVVQEQD 310 320 330 340 350 360 YNVYYGGISN GLLWPALHNL PEYIVADYDD PKVLYEHWCA YVRVNYQFAI DAVRNSRPQD 370 380 390 400 410 420 FIWIHDYHLM LTGMIMQSLD SSLEIGFFLH IPFLPPDNFF TKYRLCAFPI MRGLLRFTKV 430 440 450 460 470 480 GFQTHRDRAK FVELVGIHLP TARVTYDEKM DIHTVTYQGW SCSLGVFPVS IKNEDFLKVA 490 500 510 520 530 540 QSAETIKKAD DIRKEILGET PVDSARLLFS VERFDYTKGI KEKLLAYRRY FERHPDRIGK 550 560 570 580 590 600 DVLYQVAVTN RRSVDTYRMY QDECIQMAED INREFATDEY PNWKPLIFRT DGLQRADLVA 610 620 630 640 650 660 HYLAMDVGVV TPKKDGMNLV AKEMLVCNPS AGLVLSTGAG SEIQFTMAGL HPDDGDKCYH 670 680 690 700 710 720 RVVDVYDADH YADAFYEAAV EPEGERAAHG QRLNEFIMNN DIERWSTAFL DPGWSHLVIR 730 740 750 760 770 780 QSEIKDLDDF YSLMMRTRDV RRQIVERVLK GIPIRSHFSI SLSNTKESLL LACQPGTRTL 790 800 810 820 830 840 HLKPSLEEDE QTEPAHFDIA NELDEFEKDL NFMKFIQSDD VYNVEQFINS LQEYHPVSAD 850 860 870 880 890 900 KFRDEVIELG DVLTEADHFN FFFTDRDGTL KSYSCSYPAS IQPAYSGVIQ AQFARRCAQT 910 920 930 940 950 960 CAIVTTAPLM RIGVLDVSTI PEGYYYFGAS AGREWFIDPA NKFKDQSIPE EDLELLERVF 970 980 990 1000 1010 1020 AAISDLLEEP KFKHFTWVGS GLQKHYGHIT IAHQDAFNSV PRHQVRAIDQ KIKDIIHRID 1030 1040 1050 1060 1070 1080 PDQHTLKVKE TETDIKIFLK SESGEIFDKG QGIRLLVEHM KCDISNGTIL VCGDSSTDLP 1090 1100 1110 1120 1130 1140 MLQACLEANP SGVYTVWVTR SDELKTTVRE LCERFGNKNF VFVSCPEVLL GGMAQATIRE 1150 ISIGRPGPRA SHDSE
	Isoform b [UniParc]. Checksum: 747939ABD0ED22FF	FASTA	980	111,888
	10 20 30 40 50 60 MSVNQENMWL ANLGMNIDKK KMLRSTELLN VTDDSAPLAP ATNTLGLPLM RQALADVLFH 70 80 90 100 110 120 VIADDDIKEQ NEDEQSRNVR WGHSSVEAGG VAPSQPWEEM SLLGVLNQYN RSNYKLNPVV 130 140 150 160 170 180 VQEQDYNVYY GGISNGLLWP ALHNLPEYIV ADYDDPKVLY EHWCAYVRVN YQFAIDAVRN 190 200 210 220 230 240 SRPQDFIWIH DYHLMLTGMI MQSLDSSLEI GFFLHIPFLP PDNFFTKYRL CAFPIMRGLL 250 260 270 280 290 300 RFTKVGFQTH RDRAKFVELV GIHLPTARVT YDEKMDIHTV TYQGWSCSLG VFPVSIKNED 310 320 330 340 350 360 FLKVAQSAET IKKADDIRKE ILGETPVDSA RLLFSVERFD YTKGIKEKLL AYRRYFERHP 370 380 390 400 410 420 DRIGKDVLYQ VAVTNRRSVD TYRMYQDECI QMAEDINREF ATDEYPNWKP LIFRTDGLQR 430 440 450 460 470 480 ADLVAHYLAM DVGVVTPKKD GMNLVAKEML VCNPSAGLVL STGAGSEIQF TMAGLHPDDG 490 500 510 520 530 540 DKCYHRVVDV YDADHYADAF YEAAVEPEGE RAAHGQRLNE FIMNNDIERW STAFLDPGWS 550 560 570 580 590 600 HLVIRQSEIK DLDDFYSLMM RTRDVRRQIV ERVLKGIPIR SHFSISLSNT KESLLLACQP 610 620 630 640 650 660 GTRTLHLKPS LEEDEQTEPA HFDIANELDE FEKDLNFMKF IQSDDVYNVE QFINSLQEYH 670 680 690 700 710 720 PVSADKFRDE VIELGDVLTE ADHFNFFFTD RDGTLKSYSC SYPASIQPAY SGVIQAQFAR 730 740 750 760 770 780 RCAQTCAIVT TAPLMRIGVL DVSTIPEGYY YFGASAGREW FIDPANKFKD QSIPEEDLEL 790 800 810 820 830 840 LERVFAAISD LLEEPKFKHF TWVGSGLQKH YGHITIAHQD AFNSVPRHQV RAIDQKIKDI 850 860 870 880 890 900 IHRIDPDQHT LKVKETETDI KIFLKSESGE IFDKGQGIRL LVEHMKCDIS NGTILVCGDS 910 920 930 940 950 960 STDLPMLQAC LEANPSGVYT VWVTRSDELK TTVRELCERF GNKNFVFVSC PEVLLGGMAQ 970 980 ATIREISIGR PGPRASHDSE

References

[1]

"Dehydration-induced tps gene transcripts from an anhydrobiotic nematode contain novel spliced leaders and encode atypical GT-20 family proteins." Goyal K., Browne J.A., Burnell A.M., Tunnacliffe A. Biochimie 87:565-574(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ811568 mRNA. Translation: CAH18873.1. AJ811569 mRNA. Translation: CAH18874.1.
3D structure databases
ProteinModelPortal	Q5K2C4.
ModBase	Search...
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPS1_APHAV

Accession

Primary (citable) accession number: Q5K2C4
Secondary accession number(s): Q5K2C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	February 15, 2005
Last modified:	May 29, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)