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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2

Gene

tps-2

Organism
Aphelenchus avenae (Mycophagous nematode worm)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the production of trehalose from glucose-6-phosphate and UDP-glucose in a 2 step process.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 (EC:2.4.1.15)
Alternative name(s):
Trehalose-6-phosphate synthase 2
UDP-glucose-glucosephosphate glucosyltransferase 2
Gene namesi
Name:tps-2
OrganismiAphelenchus avenae (Mycophagous nematode worm)
Taxonomic identifieri70226 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaTylenchidaAphelenchinaAphelenchoideaAphelenchidaeAphelenchus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13031303Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2PRO_0000385174Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5K2C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the gob-1 trehalose phosphatase family.Curated

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q5K2C1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVVPGETQC EEAQPAANGS SSPVLLQPFR RPRTDSRSRL DSGSNNTTLS
60 70 80 90 100
EDCARKSILR TLKSLGVEID ESASFEAIID SGGIEKLADF WRKRDSSSEI
110 120 130 140 150
AFQGLLSVLE YCLQHSFDTN EFFEKFVNAL GYHTVQFWRA GVPYVFDSDM
160 170 180 190 200
SHGTKYRDAL LFSLTLYDVN TGKSRLKELY AAVPGIRKSL LGVHAKRFGE
210 220 230 240 250
QYHHLQRRRS VSSRGGSLRG SMDSLNDSGQ NGAEDVIGVE DEEEAQKFRG
260 270 280 290 300
KRTSISLDPA AAGEVMFTIE DGACFPSGGL ANTHFQQRVI NVSNAPPVSL
310 320 330 340 350
KREKSGEWEI KQGSGGLVSC VDPIMSVNQE NMWLANLGMN IDKKKMLRYD
360 370 380 390 400
DCLRSQEHTL ENMRSTELLN VTDDSAPLAP ATNTLGLPLM RQALADVLFH
410 420 430 440 450
VIADDDIKEQ NEDEQSRNVR EEMSLLGVLN QYNRSNYKLN PVVVQEQDYN
460 470 480 490 500
VYYGGISNGL LWPALHNLPE YIVADYDDPK VLYEHWCAYV RVNYQFAIDA
510 520 530 540 550
VRNSRPQDFI WIHDYHLMLT GMIMQSLDSS LEIGFFLHIP FLPPDNFFTK
560 570 580 590 600
YRLCAFPIMR GLLRFTKVGF QTHRDRAKFV ELVGIHLPTA RVTYDEKMDI
610 620 630 640 650
HTVTYQGWSC SLGVFPVSIK NEDFLKVAQS AETIKKADDI RKEILGETPV
660 670 680 690 700
DSARLLFSVE RFDYTKGIKE KLLAYRRYFE RHPDRIGKDV LYQVAVTNRR
710 720 730 740 750
SVDTYRMYQD ECIQMAEDIN KEFATDEYPN WKPLIFRTDG LQRADLVAHY
760 770 780 790 800
LAMDVGVVTP KKDGMNLVAK EMLVCNPSAG LVLSTGAGSE IQFTMAGLHP
810 820 830 840 850
DDGDKCYHRV VDVYDADHYA DAFYEAAVEP EAERAAHGQR LNEFIMNNDI
860 870 880 890 900
ERWSTAFLDP GWSHLVIRQS EIKDLDDFYS LMMRTRDVRR QIVERVLKGI
910 920 930 940 950
PIRSHFSISL SNAKESLLLA CQPGTRTLHL KPSLEEDEQT EPAHFDIANE
960 970 980 990 1000
LDEFEKDLNF MKFIQSDDVY NVEQFINSLQ EYHPVSADKF RDEVIELGDM
1010 1020 1030 1040 1050
LTEADHFNFF FTDRDGTLKS YSCSYPASIQ PAYSGVIQAQ FARRCAQTCA
1060 1070 1080 1090 1100
IVTTAPLMRI GVLDVSTIPE GYYYFGASAG REWFIDPANK FKDQSIPEED
1110 1120 1130 1140 1150
LELLERVFAA ISDLLEEPKF KHFTWVGSGL QKHYGHITIA HQDAFNSVPR
1160 1170 1180 1190 1200
HQVRAIDQKI KDIIHRIDPD QHTLKVKETE TDIKIFLKSE SGEIFDKGQG
1210 1220 1230 1240 1250
IRLLVEHMKC DISNGTILVC GDSSTDLPML QACLEANPSG VYTVWVTRSD
1260 1270 1280 1290 1300
ELKTTVRELC ERFGNKNFVF VSCPEVLLGG MAQATIREIS IGRPGPRASH

DSE

Note: No experimental confirmation available.

Length:1,303
Mass (Da):147,741
Last modified:February 15, 2005 - v1
Checksum:iACF8CF07481DE669
GO
Isoform b (identifier: Q5K2C1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     349-364: Missing.
     420-420: R → RWGHSSAGEAGVEPSQPW

Note: No experimental confirmation available.

Show »
Length:1,304
Mass (Da):147,513
Checksum:i0D28003A90F61398
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801Y → C in CAH18871 (PubMed:15935281).Curated
Sequence conflicti216 – 2161G → A in CAH18871 (PubMed:15935281).Curated
Sequence conflicti228 – 2292SG → A in CAH18871 (PubMed:15935281).Curated
Sequence conflicti232 – 2321G → S in CAH18871 (PubMed:15935281).Curated
Sequence conflicti269 – 2691I → T in CAH18869 (PubMed:15935281).Curated
Sequence conflicti381 – 3833ATN → TTY in CAH18871 (PubMed:15935281).Curated
Sequence conflicti441 – 4411P → A in CAH18871 (PubMed:15935281).Curated
Sequence conflicti995 – 9951I → T in CAH18869 (PubMed:15935281).Curated
Sequence conflicti1057 – 10571L → P in CAH18871 (PubMed:15935281).Curated
Sequence conflicti1084 – 10841F → L in CAH18869 (PubMed:15935281).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei349 – 36416Missing in isoform b. 1 PublicationVSP_038110Add
BLAST
Alternative sequencei420 – 4201R → RWGHSSAGEAGVEPSQPW in isoform b. 1 PublicationVSP_038111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ811571 mRNA. Translation: CAH18870.1.
AJ811570 mRNA. Translation: CAH18869.1.
AJ811572 mRNA. Translation: CAH18871.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ811571 mRNA. Translation: CAH18870.1.
AJ811570 mRNA. Translation: CAH18869.1.
AJ811572 mRNA. Translation: CAH18871.1.

3D structure databases

ProteinModelPortaliQ5K2C1.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Dehydration-induced tps gene transcripts from an anhydrobiotic nematode contain novel spliced leaders and encode atypical GT-20 family proteins."
    Goyal K., Browne J.A., Burnell A.M., Tunnacliffe A.
    Biochimie 87:565-574(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).

Entry informationi

Entry nameiTPS2_APHAV
AccessioniPrimary (citable) accession number: Q5K2C1
Secondary accession number(s): Q5K2C0, Q5K2C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 15, 2005
Last modified: October 1, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.