Q5K027 (SIAT2_ORYLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 2
Short name=Alpha 2,6-ST 2
EC=2.4.99.1

Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
ST6Gal II
Short name=ST6GalII
Sialyltransferase 2

Gene names

Name:

st6gal2

Organism

Oryzias latipes (Medaka fish) (Japanese ricefish)

Taxonomic identifier

8090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates By similarity.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Beta-galactoside alpha-2,6-sialyltransferase 2		PRO_0000314792
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 509	478	Lumenal Potential
Amino acid modifications
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Disulfide bond	237 ↔ 507		By similarity
	Disulfide bond	284 ↔ 436		By similarity
	Disulfide bond	454 ↔ 465		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5K027 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 1748521D1688AE1A	FASTA	509	57,528
	10 20 30 40 50 60 MRFSMRQWRK LVLAAILAWA LLFLGLLSYF LDNRVEEPLT PAGSLVSQHS DTRRLTSIQS 70 80 90 100 110 120 SQQQQQPVPG LRSEQGLNSI RTSHGNQPEA GVLSSSETPG NMVNHIPSSS PYQTYGSQEA 130 140 150 160 170 180 NHQNDLDPQS LAAWSSFGTQ NVDSNFNIAS QHRERASQSI FSNNVEDEEL PNEISPLVER 190 200 210 220 230 240 RADADNVVQH MWRGTVSSGM LSPRLKRAMN DYINANKHHV SYQRHRKVAR SAKELLCQMK 250 260 270 280 290 300 NQSQLRTVDG SEQPFSSLGW ADFVPLVPLQ RWNKQRGGRS FRTCAVVSSA GAILHSGLGK 310 320 330 340 350 360 EIDSHDAVLR FNAAPTEGYE QDVGTKTTIR IINSQILANP KHEFKTSSIY KNITLVAWDP 370 380 390 400 410 420 APYTLNLDEW FASPDYDLFG PYVEHRKNHA EQLFYILHPS YLWQLWDLIQ SNTQEKIQPN 430 440 450 460 470 480 PPSSGFIGIL TMMALCDKLH VYEYIPSMRQ TDLCHYHENY YDAACTLGAY HPLIYEKNLI 490 500 RRINLGSEKD LLKKGRVTLP GFSTLTCGA

References

[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ871601 mRNA. Translation: CAI39644.1.
RefSeq	NP_001098242.1. NM_001104772.1.
UniGene	Ola.9871.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSORLT00000015722; ENSORLP00000015721; ENSORLG00000012556.
GeneID	100049381.
Organism-specific databases
CTD	100049381.
Phylogenomic databases
eggNOG	fiNOG08912.
GeneTree	ENSGT00550000074444.
HOVERGEN	HBG052853.
InParanoid	Q5K027.
Enzyme and pathway databases
BRENDA	2.4.99.1. 3199.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIAT2_ORYLA

Accession

Primary (citable) accession number: Q5K027

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program