Q5JY77 (GASP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor-associated sorting protein 1
Short name=GASP-1

Gene names

Name:	GPRASP1
Synonyms:	GASP, KIAA0443

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes. Ref.5 Ref.6
Subunit structure	Interacts with cytoplasmic tails of a variety of G-protein coupled receptors such as D2 dopamine receptor/DRD2 By similarity, delta opioid receptor/OPRD1, beta-2 adrenergic receptor/ADRB2 and D4 dopamine receptor/DRD4. Interacts with PER1. Ref.5 Ref.6 Ref.7
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra. Ref.5 Ref.7
Sequence similarities	Belongs to the GPRASP family.
Sequence caution	The sequence BAA23715.3 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OPRD1	P41143	2	EBI-2514717,EBI-2624456

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1395	1395	G-protein coupled receptor-associated sorting protein 1		PRO_0000239050
Regions
	Region	899 – 1395	497	OPRD1-binding
	Compositional bias	506 – 978	473	Glu-rich
Natural variations
	Natural variant	315	1	A → G. Ref.1 Corresponds to variant rs17339512 [ dbSNP | Ensembl ].		VAR_026579
	Natural variant	779	1	I → V. Corresponds to variant rs17292748 [ dbSNP | Ensembl ].		VAR_049263
	Natural variant	1093	1	P → S. Corresponds to variant rs2235804 [ dbSNP | Ensembl ].		VAR_049264

Sequences

	Sequence		Length	Mass (Da)
	Q5JY77 [UniParc]. Last modified February 20, 2007. Version 3. Checksum: 89733CB9A59BC151	FASTA	1,395	156,865
	10 20 30 40 50 60 MTGAEIESGA QVKPEKKPGE EVVGGAEIEN DVPLVVRPKV RTQAQIMPGA RPKNKSKVMP 70 80 90 100 110 120 GASTKVETSA VGGARPKSKA KAIPVSRFKE EAQMWAQPRF GAERLSKTER NSQTNIIASP 130 140 150 160 170 180 LVSTDSVLVA KTKYLSEDRE LVNTDTESFP RRKAHYQAGF QPSFRSKEET NMGSWCCPRP 190 200 210 220 230 240 TSKQEASPNS DFKWVDKSVS SLFWSGDEVT AKFHPGNRVK DSNRSMHMAN QEANTMSRSQ 250 260 270 280 290 300 TNQELYIASS SGSEDESVKT PWFWARDKTN TWSGPREDPN SRSRFRSKKE VYVESSSGSE 310 320 330 340 350 360 HEDHLESWFG AGKEAKFRSK MRAGKEANNR ARHRAKREAC IDFMPGSIDV IKKESCFWPE 370 380 390 400 410 420 ENANTFSRPM IKKEARARAM TKEEAKTKAR ARAKQEARSE EEALIGTWFW ATDESSMADE 430 440 450 460 470 480 ASIESSLQVE DESIIGSWFW TEEEASMGTG ASSKSRPRTD GERIGDSLFG AREKTSMKTG 490 500 510 520 530 540 AEATSESILA ADDEQVIIGS WFWAGEEVNQ EAEEETIFGS WFWVIDAASV ESGVGVSCES 550 560 570 580 590 600 RTRSEEEEVI GPWFWSGEQV DIEAGIGEEA RPGAEEETIF GSWFWAENQT YMDCRAETSC 610 620 630 640 650 660 DTMQGAEEEE PIIGSWFWTR VEACVEGDVN SKSSLEDKEE AMIPCFGAKE EVSMKHGTGV 670 680 690 700 710 720 RCRFMAGAEE TNNKSCFWAE KEPCMYPAGG GSWKSRPEEE EDIVNSWFWS RKYTKPEAII 730 740 750 760 770 780 GSWLWATEES NIDGTGEKAK LLTEEETIIN SWFWKEDEAI SEATDREESR PEAEEGDIIG 790 800 810 820 830 840 SWFWAGEEDR LEPAAETREE DRLAAEKEGI VGSWFGAREE TIRREAGSCS KSSPKAEEEE 850 860 870 880 890 900 VIIGSWFWEE EASPEAVAGV GFESKPGTEE EEITVGSWFW PEEEASIQAG SQAVEEMESE 910 920 930 940 950 960 TEEETIFGSW FWDGKEVSEE AGPCCVSKPE DDEEMIVESW FWSRDKAIKE TGTVATCESK 970 980 990 1000 1010 1020 PENEEGAIVG SWFEAEDEVD NRTDNGSNCG SRTLADEDEA IVGSWFWAGD EAHFESNPSP 1030 1040 1050 1060 1070 1080 VFRAICRSTC SVEQEPDPSR RPQSWEEVTV QFKPGPWGRV GFPSISPFRF PKEAASLFCE 1090 1100 1110 1120 1130 1140 MFGGKPRNMV LSPEGEDQES LLQPDQPSPE FPFQYDPSYR SVQEIREHLR AKESTEPESS 1150 1160 1170 1180 1190 1200 SCNCIQCELK IGSEEFEELL LLMEKIRDPF IHEISKIAMG MRSASQFTRD FIRDSGVVSL 1210 1220 1230 1240 1250 1260 IETLLNYPSS RVRTSFLENM IRMAPPYPNL NIIQTYICKV CEETLAYSVD SPEQLSGIRM 1270 1280 1290 1300 1310 1320 IRHLTTTTDY HTLVANYMSG FLSLLATGNA KTRFHVLKML LNLSENLFMT KELLSAEAVS 1330 1340 1350 1360 1370 1380 EFIGLFNREE TNDNIQIVLA IFENIGNNIK KETVFSDDDF NIEPLISAFH KVEKFAKELQ 1390 GKTDNQNDPE GDQEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro." Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-315. Tissue: Brain.
[2]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Completely sequenced partial cDNA clone from a human heart library." Patzak D. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1362-1395. Tissue: Heart.
[5]	"Modulation of postendocytic sorting of G protein-coupled receptors." Whistler J.L., Enquist J., Marley A., Fong J., Gladher F., Tsuruda P., Murray S.R., Von Zastrow M. Science 297:615-620(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH OPRD1; ADRB2 AND DRD4, DOMAIN OPRD1-BINDING, TISSUE SPECIFICITY.
[6]	"A library of 7TM receptor C-terminal tails. Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated sorting protein (GASP)." Heydorn A., Soendergaard B.P., Ersboell B., Holst B., Nielsen F.C., Haft C.R., Whistler J., Schwartz T.W. J. Biol. Chem. 279:54291-54303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH G PROTEIN-COUPLED RECEPTORS, SUBCELLULAR LOCATION.
[7]	"Identification of a novel family of G protein-coupled receptor associated sorting proteins." Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L. J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH OPRD1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007903 mRNA. Translation: BAA23715.3. Different initiation. AL035427 Genomic DNA. Translation: CAB55683.1. BC114552 mRNA. Translation: AAI14553.1. AY044233 mRNA. Translation: AAK95578.1.
IPI	IPI00060549.
PIR	T00068.
RefSeq	NP_001092880.1. NM_001099410.1. NP_001092881.1. NM_001099411.1. NP_001171656.1. NM_001184727.1. NP_055525.3. NM_014710.4.
UniGene	Hs.729265.
3D structure databases
ProteinModelPortal	Q5JY77.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5JY77. 17 interactions.
MINT	MINT-1413443.
STRING	9606.ENSP00000355146.
PTM databases
PhosphoSite	Q5JY77.
Polymorphism databases
DMDM	126302546.
Proteomic databases
PaxDb	Q5JY77.
PRIDE	Q5JY77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361600; ENSP00000355146; ENSG00000198932. ENST00000415986; ENSP00000393691; ENSG00000198932. ENST00000444152; ENSP00000409420; ENSG00000198932. ENST00000537097; ENSP00000445683; ENSG00000198932.
GeneID	9737.
KEGG	hsa:9737.
UCSC	uc004eji.4. human.
Organism-specific databases
CTD	9737.
GeneCards	GC0XP101906.
HGNC	HGNC:24834. GPRASP1.
HPA	HPA000161.
MIM	300417. gene.
neXtProt	NX_Q5JY77.
PharmGKB	PA134970616.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG136667.
HOGENOM	HOG000231409.
HOVERGEN	HBG057167.
InParanoid	Q5JY77.
OMA	FGSWFWA.
OrthoDB	EOG47PX56.
Gene expression databases
Bgee	Q5JY77.
CleanEx	HS_GPRASP1.
Genevestigator	Q5JY77.
GermOnline	ENSG00000198932. Homo sapiens.
Family and domain databases
Gene3D	1.25.10.10. 2 hits.
InterPro	IPR011989. ARM-like. IPR006911. ARM-rpt_dom. IPR016024. ARM-type_fold. [Graphical view]
Pfam	PF04826. Arm_2. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	9737.
NextBio	36640.
SOURCE	Search...

Entry information

Entry name

GASP1_HUMAN

Accession

Primary (citable) accession number: Q5JY77
Secondary accession number(s): O43168, Q96LA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 20, 2007
Last modified:	April 3, 2013
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.