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Protein

G-protein coupled receptor-associated sorting protein 1

Gene

GPRASP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.3 Publications

GO - Biological processi

  1. endosome to lysosome transport Source: UniProtKB
  2. G-protein coupled receptor catabolic process Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor-associated sorting protein 1
Short name:
GASP-1
Gene namesi
Name:GPRASP1
Synonyms:GASP, KIAA0443
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:24834. GPRASP1.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134970616.

Polymorphism and mutation databases

BioMutaiGPRASP1.
DMDMi126302546.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13951395G-protein coupled receptor-associated sorting protein 1PRO_0000239050Add
BLAST

Proteomic databases

MaxQBiQ5JY77.
PaxDbiQ5JY77.
PRIDEiQ5JY77.

PTM databases

PhosphoSiteiQ5JY77.

Expressioni

Tissue specificityi

Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra.2 Publications

Gene expression databases

BgeeiQ5JY77.
CleanExiHS_GPRASP1.
GenevestigatoriQ5JY77.

Organism-specific databases

HPAiHPA000161.

Interactioni

Subunit structurei

Interacts with cytoplasmic tails of a variety of G-protein coupled receptors such as D2 dopamine receptor/DRD2 (By similarity), delta opioid receptor/OPRD1, beta-2 adrenergic receptor/ADRB2 and D4 dopamine receptor/DRD4. Interacts with PER1. Interacts with BECN2; the interaction is direct.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BECN1P1A8MW954EBI-2514717,EBI-8839517
OPRD1P411432EBI-2514717,EBI-2624456

Protein-protein interaction databases

BioGridi115086. 29 interactions.
IntActiQ5JY77. 18 interactions.
MINTiMINT-1413443.
STRINGi9606.ENSP00000355146.

Structurei

3D structure databases

ProteinModelPortaliQ5JY77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni899 – 1395497OPRD1-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi506 – 978473Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the GPRASP family.Curated

Phylogenomic databases

eggNOGiNOG136667.
GeneTreeiENSGT00550000074488.
HOGENOMiHOG000231409.
HOVERGENiHBG057167.
InParanoidiQ5JY77.
OMAiMIQTYVC.
OrthoDBiEOG70PBZ0.
PhylomeDBiQ5JY77.
TreeFamiTF335652.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR006911. ARM-rpt_dom.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF04826. Arm_2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5JY77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGAEIESGA QVKPEKKPGE EVVGGAEIEN DVPLVVRPKV RTQAQIMPGA
60 70 80 90 100
RPKNKSKVMP GASTKVETSA VGGARPKSKA KAIPVSRFKE EAQMWAQPRF
110 120 130 140 150
GAERLSKTER NSQTNIIASP LVSTDSVLVA KTKYLSEDRE LVNTDTESFP
160 170 180 190 200
RRKAHYQAGF QPSFRSKEET NMGSWCCPRP TSKQEASPNS DFKWVDKSVS
210 220 230 240 250
SLFWSGDEVT AKFHPGNRVK DSNRSMHMAN QEANTMSRSQ TNQELYIASS
260 270 280 290 300
SGSEDESVKT PWFWARDKTN TWSGPREDPN SRSRFRSKKE VYVESSSGSE
310 320 330 340 350
HEDHLESWFG AGKEAKFRSK MRAGKEANNR ARHRAKREAC IDFMPGSIDV
360 370 380 390 400
IKKESCFWPE ENANTFSRPM IKKEARARAM TKEEAKTKAR ARAKQEARSE
410 420 430 440 450
EEALIGTWFW ATDESSMADE ASIESSLQVE DESIIGSWFW TEEEASMGTG
460 470 480 490 500
ASSKSRPRTD GERIGDSLFG AREKTSMKTG AEATSESILA ADDEQVIIGS
510 520 530 540 550
WFWAGEEVNQ EAEEETIFGS WFWVIDAASV ESGVGVSCES RTRSEEEEVI
560 570 580 590 600
GPWFWSGEQV DIEAGIGEEA RPGAEEETIF GSWFWAENQT YMDCRAETSC
610 620 630 640 650
DTMQGAEEEE PIIGSWFWTR VEACVEGDVN SKSSLEDKEE AMIPCFGAKE
660 670 680 690 700
EVSMKHGTGV RCRFMAGAEE TNNKSCFWAE KEPCMYPAGG GSWKSRPEEE
710 720 730 740 750
EDIVNSWFWS RKYTKPEAII GSWLWATEES NIDGTGEKAK LLTEEETIIN
760 770 780 790 800
SWFWKEDEAI SEATDREESR PEAEEGDIIG SWFWAGEEDR LEPAAETREE
810 820 830 840 850
DRLAAEKEGI VGSWFGAREE TIRREAGSCS KSSPKAEEEE VIIGSWFWEE
860 870 880 890 900
EASPEAVAGV GFESKPGTEE EEITVGSWFW PEEEASIQAG SQAVEEMESE
910 920 930 940 950
TEEETIFGSW FWDGKEVSEE AGPCCVSKPE DDEEMIVESW FWSRDKAIKE
960 970 980 990 1000
TGTVATCESK PENEEGAIVG SWFEAEDEVD NRTDNGSNCG SRTLADEDEA
1010 1020 1030 1040 1050
IVGSWFWAGD EAHFESNPSP VFRAICRSTC SVEQEPDPSR RPQSWEEVTV
1060 1070 1080 1090 1100
QFKPGPWGRV GFPSISPFRF PKEAASLFCE MFGGKPRNMV LSPEGEDQES
1110 1120 1130 1140 1150
LLQPDQPSPE FPFQYDPSYR SVQEIREHLR AKESTEPESS SCNCIQCELK
1160 1170 1180 1190 1200
IGSEEFEELL LLMEKIRDPF IHEISKIAMG MRSASQFTRD FIRDSGVVSL
1210 1220 1230 1240 1250
IETLLNYPSS RVRTSFLENM IRMAPPYPNL NIIQTYICKV CEETLAYSVD
1260 1270 1280 1290 1300
SPEQLSGIRM IRHLTTTTDY HTLVANYMSG FLSLLATGNA KTRFHVLKML
1310 1320 1330 1340 1350
LNLSENLFMT KELLSAEAVS EFIGLFNREE TNDNIQIVLA IFENIGNNIK
1360 1370 1380 1390
KETVFSDDDF NIEPLISAFH KVEKFAKELQ GKTDNQNDPE GDQEN
Length:1,395
Mass (Da):156,865
Last modified:February 20, 2007 - v3
Checksum:i89733CB9A59BC151
GO

Sequence cautioni

The sequence BAA23715.3 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti315 – 3151A → G.1 Publication
Corresponds to variant rs17339512 [ dbSNP | Ensembl ].
VAR_026579
Natural varianti779 – 7791I → V.
Corresponds to variant rs17292748 [ dbSNP | Ensembl ].
VAR_049263
Natural varianti1093 – 10931P → S.
Corresponds to variant rs2235804 [ dbSNP | Ensembl ].
VAR_049264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007903 mRNA. Translation: BAA23715.3. Different initiation.
AL035427 Genomic DNA. Translation: CAB55683.1.
BC114552 mRNA. Translation: AAI14553.1.
AY044233 mRNA. Translation: AAK95578.1.
CCDSiCCDS35352.1.
PIRiT00068.
RefSeqiNP_001092880.1. NM_001099410.1.
NP_001092881.1. NM_001099411.1.
NP_001171656.1. NM_001184727.1.
NP_055525.3. NM_014710.4.
UniGeneiHs.729265.
Hs.731996.

Genome annotation databases

EnsembliENST00000361600; ENSP00000355146; ENSG00000198932.
ENST00000415986; ENSP00000393691; ENSG00000198932.
ENST00000444152; ENSP00000409420; ENSG00000198932.
ENST00000537097; ENSP00000445683; ENSG00000198932.
GeneIDi9737.
KEGGihsa:9737.
UCSCiuc004eji.4. human.

Polymorphism and mutation databases

BioMutaiGPRASP1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007903 mRNA. Translation: BAA23715.3. Different initiation.
AL035427 Genomic DNA. Translation: CAB55683.1.
BC114552 mRNA. Translation: AAI14553.1.
AY044233 mRNA. Translation: AAK95578.1.
CCDSiCCDS35352.1.
PIRiT00068.
RefSeqiNP_001092880.1. NM_001099410.1.
NP_001092881.1. NM_001099411.1.
NP_001171656.1. NM_001184727.1.
NP_055525.3. NM_014710.4.
UniGeneiHs.729265.
Hs.731996.

3D structure databases

ProteinModelPortaliQ5JY77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115086. 29 interactions.
IntActiQ5JY77. 18 interactions.
MINTiMINT-1413443.
STRINGi9606.ENSP00000355146.

PTM databases

PhosphoSiteiQ5JY77.

Polymorphism and mutation databases

BioMutaiGPRASP1.
DMDMi126302546.

Proteomic databases

MaxQBiQ5JY77.
PaxDbiQ5JY77.
PRIDEiQ5JY77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361600; ENSP00000355146; ENSG00000198932.
ENST00000415986; ENSP00000393691; ENSG00000198932.
ENST00000444152; ENSP00000409420; ENSG00000198932.
ENST00000537097; ENSP00000445683; ENSG00000198932.
GeneIDi9737.
KEGGihsa:9737.
UCSCiuc004eji.4. human.

Organism-specific databases

CTDi9737.
GeneCardsiGC0XP101906.
HGNCiHGNC:24834. GPRASP1.
HPAiHPA000161.
MIMi300417. gene.
neXtProtiNX_Q5JY77.
PharmGKBiPA134970616.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG136667.
GeneTreeiENSGT00550000074488.
HOGENOMiHOG000231409.
HOVERGENiHBG057167.
InParanoidiQ5JY77.
OMAiMIQTYVC.
OrthoDBiEOG70PBZ0.
PhylomeDBiQ5JY77.
TreeFamiTF335652.

Miscellaneous databases

GeneWikiiGPRASP1_(gene).
GenomeRNAii9737.
NextBioi36640.
PROiQ5JY77.
SOURCEiSearch...

Gene expression databases

BgeeiQ5JY77.
CleanExiHS_GPRASP1.
GenevestigatoriQ5JY77.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR006911. ARM-rpt_dom.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF04826. Arm_2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
    Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-315.
    Tissue: Brain.
  2. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Completely sequenced partial cDNA clone from a human heart library."
    Patzak D.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1362-1395.
    Tissue: Heart.
  5. "Modulation of postendocytic sorting of G protein-coupled receptors."
    Whistler J.L., Enquist J., Marley A., Fong J., Gladher F., Tsuruda P., Murray S.R., Von Zastrow M.
    Science 297:615-620(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH OPRD1; ADRB2 AND DRD4, DOMAIN OPRD1-BINDING, TISSUE SPECIFICITY.
  6. "A library of 7TM receptor C-terminal tails. Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated sorting protein (GASP)."
    Heydorn A., Soendergaard B.P., Ersboell B., Holst B., Nielsen F.C., Haft C.R., Whistler J., Schwartz T.W.
    J. Biol. Chem. 279:54291-54303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH G PROTEIN-COUPLED RECEPTORS, SUBCELLULAR LOCATION.
  7. "Identification of a novel family of G protein-coupled receptor associated sorting proteins."
    Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
    J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH OPRD1.
  8. "Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism."
    He C., Wei Y., Sun K., Li B., Dong X., Zou Z., Liu Y., Kinch L.N., Khan S., Sinha S., Xavier R.J., Grishin N.V., Xiao G., Eskelinen E.L., Scherer P.E., Whistler J.L., Levine B.
    Cell 154:1085-1099(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BECN2.

Entry informationi

Entry nameiGASP1_HUMAN
AccessioniPrimary (citable) accession number: Q5JY77
Secondary accession number(s): O43168, Q96LA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 20, 2007
Last modified: April 29, 2015
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.