Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5JY77 (GASP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor-associated sorting protein 1

Short name=GASP-1
Gene names
Name:GPRASP1
Synonyms:GASP, KIAA0443
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN1P1/BECN2. Ref.5 Ref.6 Ref.8

Subunit structure

Interacts with cytoplasmic tails of a variety of G-protein coupled receptors such as D2 dopamine receptor/DRD2 By similarity, delta opioid receptor/OPRD1, beta-2 adrenergic receptor/ADRB2 and D4 dopamine receptor/DRD4. Interacts with PER1. Interacts with BECN1P1/BECN2; the interaction is direct. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm Ref.6.

Tissue specificity

Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra. Ref.5 Ref.7

Sequence similarities

Belongs to the GPRASP family.

Sequence caution

The sequence BAA23715.3 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor catabolic process

Inferred from mutant phenotype Ref.8. Source: UniProtKB

endosome to lysosome transport

Inferred from mutant phenotype Ref.8. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BECN1P1A8MW954EBI-2514717,EBI-8839517
OPRD1P411432EBI-2514717,EBI-2624456

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13951395G-protein coupled receptor-associated sorting protein 1
PRO_0000239050

Regions

Region899 – 1395497OPRD1-binding
Compositional bias506 – 978473Glu-rich

Natural variations

Natural variant3151A → G. Ref.1
Corresponds to variant rs17339512 [ dbSNP | Ensembl ].
VAR_026579
Natural variant7791I → V.
Corresponds to variant rs17292748 [ dbSNP | Ensembl ].
VAR_049263
Natural variant10931P → S.
Corresponds to variant rs2235804 [ dbSNP | Ensembl ].
VAR_049264

Sequences

Sequence LengthMass (Da)Tools
Q5JY77 [UniParc].

Last modified February 20, 2007. Version 3.
Checksum: 89733CB9A59BC151

FASTA1,395156,865
        10         20         30         40         50         60 
MTGAEIESGA QVKPEKKPGE EVVGGAEIEN DVPLVVRPKV RTQAQIMPGA RPKNKSKVMP 

        70         80         90        100        110        120 
GASTKVETSA VGGARPKSKA KAIPVSRFKE EAQMWAQPRF GAERLSKTER NSQTNIIASP 

       130        140        150        160        170        180 
LVSTDSVLVA KTKYLSEDRE LVNTDTESFP RRKAHYQAGF QPSFRSKEET NMGSWCCPRP 

       190        200        210        220        230        240 
TSKQEASPNS DFKWVDKSVS SLFWSGDEVT AKFHPGNRVK DSNRSMHMAN QEANTMSRSQ 

       250        260        270        280        290        300 
TNQELYIASS SGSEDESVKT PWFWARDKTN TWSGPREDPN SRSRFRSKKE VYVESSSGSE 

       310        320        330        340        350        360 
HEDHLESWFG AGKEAKFRSK MRAGKEANNR ARHRAKREAC IDFMPGSIDV IKKESCFWPE 

       370        380        390        400        410        420 
ENANTFSRPM IKKEARARAM TKEEAKTKAR ARAKQEARSE EEALIGTWFW ATDESSMADE 

       430        440        450        460        470        480 
ASIESSLQVE DESIIGSWFW TEEEASMGTG ASSKSRPRTD GERIGDSLFG AREKTSMKTG 

       490        500        510        520        530        540 
AEATSESILA ADDEQVIIGS WFWAGEEVNQ EAEEETIFGS WFWVIDAASV ESGVGVSCES 

       550        560        570        580        590        600 
RTRSEEEEVI GPWFWSGEQV DIEAGIGEEA RPGAEEETIF GSWFWAENQT YMDCRAETSC 

       610        620        630        640        650        660 
DTMQGAEEEE PIIGSWFWTR VEACVEGDVN SKSSLEDKEE AMIPCFGAKE EVSMKHGTGV 

       670        680        690        700        710        720 
RCRFMAGAEE TNNKSCFWAE KEPCMYPAGG GSWKSRPEEE EDIVNSWFWS RKYTKPEAII 

       730        740        750        760        770        780 
GSWLWATEES NIDGTGEKAK LLTEEETIIN SWFWKEDEAI SEATDREESR PEAEEGDIIG 

       790        800        810        820        830        840 
SWFWAGEEDR LEPAAETREE DRLAAEKEGI VGSWFGAREE TIRREAGSCS KSSPKAEEEE 

       850        860        870        880        890        900 
VIIGSWFWEE EASPEAVAGV GFESKPGTEE EEITVGSWFW PEEEASIQAG SQAVEEMESE 

       910        920        930        940        950        960 
TEEETIFGSW FWDGKEVSEE AGPCCVSKPE DDEEMIVESW FWSRDKAIKE TGTVATCESK 

       970        980        990       1000       1010       1020 
PENEEGAIVG SWFEAEDEVD NRTDNGSNCG SRTLADEDEA IVGSWFWAGD EAHFESNPSP 

      1030       1040       1050       1060       1070       1080 
VFRAICRSTC SVEQEPDPSR RPQSWEEVTV QFKPGPWGRV GFPSISPFRF PKEAASLFCE 

      1090       1100       1110       1120       1130       1140 
MFGGKPRNMV LSPEGEDQES LLQPDQPSPE FPFQYDPSYR SVQEIREHLR AKESTEPESS 

      1150       1160       1170       1180       1190       1200 
SCNCIQCELK IGSEEFEELL LLMEKIRDPF IHEISKIAMG MRSASQFTRD FIRDSGVVSL 

      1210       1220       1230       1240       1250       1260 
IETLLNYPSS RVRTSFLENM IRMAPPYPNL NIIQTYICKV CEETLAYSVD SPEQLSGIRM 

      1270       1280       1290       1300       1310       1320 
IRHLTTTTDY HTLVANYMSG FLSLLATGNA KTRFHVLKML LNLSENLFMT KELLSAEAVS 

      1330       1340       1350       1360       1370       1380 
EFIGLFNREE TNDNIQIVLA IFENIGNNIK KETVFSDDDF NIEPLISAFH KVEKFAKELQ 

      1390 
GKTDNQNDPE GDQEN 

« Hide

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-315.
Tissue: Brain.
[2]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Completely sequenced partial cDNA clone from a human heart library."
Patzak D.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1362-1395.
Tissue: Heart.
[5]"Modulation of postendocytic sorting of G protein-coupled receptors."
Whistler J.L., Enquist J., Marley A., Fong J., Gladher F., Tsuruda P., Murray S.R., Von Zastrow M.
Science 297:615-620(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH OPRD1; ADRB2 AND DRD4, DOMAIN OPRD1-BINDING, TISSUE SPECIFICITY.
[6]"A library of 7TM receptor C-terminal tails. Interactions with the proposed post-endocytic sorting proteins ERM-binding phosphoprotein 50 (EBP50), N-ethylmaleimide-sensitive factor (NSF), sorting nexin 1 (SNX1), and G protein-coupled receptor-associated sorting protein (GASP)."
Heydorn A., Soendergaard B.P., Ersboell B., Holst B., Nielsen F.C., Haft C.R., Whistler J., Schwartz T.W.
J. Biol. Chem. 279:54291-54303(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH G PROTEIN-COUPLED RECEPTORS, SUBCELLULAR LOCATION.
[7]"Identification of a novel family of G protein-coupled receptor associated sorting proteins."
Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INTERACTION WITH OPRD1.
[8]"Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism."
He C., Wei Y., Sun K., Li B., Dong X., Zou Z., Liu Y., Kinch L.N., Khan S., Sinha S., Xavier R.J., Grishin N.V., Xiao G., Eskelinen E.L., Scherer P.E., Whistler J.L., Levine B.
Cell 154:1085-1099(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BECN1P1/BECN2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007903 mRNA. Translation: BAA23715.3. Different initiation.
AL035427 Genomic DNA. Translation: CAB55683.1.
BC114552 mRNA. Translation: AAI14553.1.
AY044233 mRNA. Translation: AAK95578.1.
PIRT00068.
RefSeqNP_001092880.1. NM_001099410.1.
NP_001092881.1. NM_001099411.1.
NP_001171656.1. NM_001184727.1.
NP_055525.3. NM_014710.4.
UniGeneHs.729265.
Hs.731996.

3D structure databases

ProteinModelPortalQ5JY77.
SMRQ5JY77. Positions 1157-1212.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115086. 28 interactions.
IntActQ5JY77. 18 interactions.
MINTMINT-1413443.
STRING9606.ENSP00000355146.

PTM databases

PhosphoSiteQ5JY77.

Polymorphism databases

DMDM126302546.

Proteomic databases

PaxDbQ5JY77.
PRIDEQ5JY77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361600; ENSP00000355146; ENSG00000198932.
ENST00000415986; ENSP00000393691; ENSG00000198932.
ENST00000444152; ENSP00000409420; ENSG00000198932.
ENST00000537097; ENSP00000445683; ENSG00000198932.
GeneID9737.
KEGGhsa:9737.
UCSCuc004eji.4. human.

Organism-specific databases

CTD9737.
GeneCardsGC0XP101906.
HGNCHGNC:24834. GPRASP1.
HPAHPA000161.
MIM300417. gene.
neXtProtNX_Q5JY77.
PharmGKBPA134970616.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG136667.
HOGENOMHOG000231409.
HOVERGENHBG057167.
InParanoidQ5JY77.
OMAMIQTYVC.
OrthoDBEOG70PBZ0.
PhylomeDBQ5JY77.
TreeFamTF335652.

Gene expression databases

BgeeQ5JY77.
CleanExHS_GPRASP1.
GenevestigatorQ5JY77.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR011989. ARM-like.
IPR006911. ARM-rpt_dom.
IPR016024. ARM-type_fold.
[Graphical view]
PfamPF04826. Arm_2. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

GeneWikiGPRASP1_(gene).
GenomeRNAi9737.
NextBio36640.
PROQ5JY77.
SOURCESearch...

Entry information

Entry nameGASP1_HUMAN
AccessionPrimary (citable) accession number: Q5JY77
Secondary accession number(s): O43168, Q96LA1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 20, 2007
Last modified: April 16, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM