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Protein

Glycine receptor subunit alpha-4

Gene

GLRA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei296 – 2961Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-HSA-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-4
Gene namesi
Name:GLRA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:31715. GLRA4.

Subcellular locationi

  • Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cell junctionsynapse By similarity
  • Perikaryon By similarity
  • Cell projectiondendrite By similarity
  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 257229ExtracellularBy similarityAdd
BLAST
Transmembranei258 – 27922Helical; Name=1By similarityAdd
BLAST
Topological domaini280 – 2845CytoplasmicBy similarity
Transmembranei285 – 30521Helical; Name=2By similarityAdd
BLAST
Topological domaini306 – 31611ExtracellularBy similarityAdd
BLAST
Transmembranei317 – 33721Helical; Name=3By similarityAdd
BLAST
Topological domaini338 – 41780CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671731.

Polymorphism and mutation databases

BioMutaiGLRA4.
DMDMi262527577.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 417389Glycine receptor subunit alpha-4PRO_0000338609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Disulfide bondi172 ↔ 186By similarity
Disulfide bondi233 ↔ 244By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5JXX5.
PRIDEiQ5JXX5.

PTM databases

PhosphoSiteiQ5JXX5.

Expressioni

Tissue specificityi

Detected in trachea smooth muscle.1 Publication

Gene expression databases

BgeeiQ5JXX5.
CleanExiHS_GLRA4.
ExpressionAtlasiQ5JXX5. baseline and differential.

Organism-specific databases

HPAiHPA044759.

Interactioni

Subunit structurei

Homopentamer (in vitro). Pentamer composed of alpha and beta subunits.By similarity

Protein-protein interaction databases

BioGridi137537. 1 interaction.
STRINGi9606.ENSP00000361700.

Structurei

3D structure databases

ProteinModelPortaliQ5JXX5.
SMRiQ5JXX5. Positions 43-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2426Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ5JXX5.
KOiK05271.
OMAiARDGGPM.
OrthoDBiEOG712TVZ.
PhylomeDBiQ5JXX5.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JXX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLVPATLS FLLLWTLPGQ VLLRVALAKE EVKSGTKGSQ PMSPSDFLDK
60 70 80 90 100
LMGRTSGYDA RIRPNFKGPP VNVTCNIFIN SFSSITKTTM DYRVNVFLRQ
110 120 130 140 150
QWNDPRLSYR EYPDDSLDLD PSMLDSIWKP DLFFANEKGA NFHEVTTDNK
160 170 180 190 200
LLRIFKNGNV LYSIRLTLIL SCLMDLKNFP MDIQTCTMQL ESFGYTMKDL
210 220 230 240 250
VFEWLEDAPA VQVAEGLTLP QFILRDEKDL GCCTKHYNTG KFTCIEVKFH
260 270 280 290 300
LERQMGYYLI QMYIPSLLIV ILSWVSFWIN MDAAPARVGL GITTVLTMTT
310 320 330 340 350
QSSGSRASLP KVSYVKAIDI WMAVCLLFVF AALLEYAAIN FVSRQHKEFI
360 370 380 390 400
RLRRRQRRQR LEEDIIQESR FYFRGYGLGH CLQARDGGPM EGSGIYSPQP
410
PAPLLREGET TRKLYVD
Length:417
Mass (Da):47,728
Last modified:November 3, 2009 - v3
Checksum:i7F3B1A5B26B7F0E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93848, AL049610 Genomic DNA. Translation: CAI41994.2.
AL049610, Z93848 Genomic DNA. Translation: CAI42158.2.
CCDSiCCDS43980.2.
RefSeqiNP_001019623.2. NM_001024452.2.
UniGeneiHs.533289.

Genome annotation databases

EnsembliENST00000372617; ENSP00000361700; ENSG00000188828.
GeneIDi441509.
KEGGihsa:441509.
UCSCiuc011mse.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93848, AL049610 Genomic DNA. Translation: CAI41994.2.
AL049610, Z93848 Genomic DNA. Translation: CAI42158.2.
CCDSiCCDS43980.2.
RefSeqiNP_001019623.2. NM_001024452.2.
UniGeneiHs.533289.

3D structure databases

ProteinModelPortaliQ5JXX5.
SMRiQ5JXX5. Positions 43-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi137537. 1 interaction.
STRINGi9606.ENSP00000361700.

PTM databases

PhosphoSiteiQ5JXX5.

Polymorphism and mutation databases

BioMutaiGLRA4.
DMDMi262527577.

Proteomic databases

PaxDbiQ5JXX5.
PRIDEiQ5JXX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372617; ENSP00000361700; ENSG00000188828.
GeneIDi441509.
KEGGihsa:441509.
UCSCiuc011mse.3. human.

Organism-specific databases

CTDi441509.
GeneCardsiGLRA4.
HGNCiHGNC:31715. GLRA4.
HPAiHPA044759.
neXtProtiNX_Q5JXX5.
PharmGKBiPA142671731.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ5JXX5.
KOiK05271.
OMAiARDGGPM.
OrthoDBiEOG712TVZ.
PhylomeDBiQ5JXX5.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-HSA-975298. Ligand-gated ion channel transport.

Miscellaneous databases

GeneWikiiGLRA4.
GenomeRNAii441509.
NextBioi110250.
PROiQ5JXX5.

Gene expression databases

BgeeiQ5JXX5.
CleanExiHS_GLRA4.
ExpressionAtlasiQ5JXX5. baseline and differential.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Novel expression of a functional glycine receptor chloride channel that attenuates contraction in airway smooth muscle."
    Yim P.D., Gallos G., Xu D., Zhang Y., Emala C.W.
    FASEB J. 25:1706-1717(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGLRA4_HUMAN
AccessioniPrimary (citable) accession number: Q5JXX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: November 3, 2009
Last modified: April 13, 2016
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.By similarity

Caution

Is shorter at the C-terminus than its mouse ortholog due to the presence of a premature stop codon. As a consequence, it lacks the fourth transmembrane span.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.