Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Migration and invasion-inhibitory protein

Gene

MIIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits glioma cells invasion and down-regulates adhesion- and motility-associated genes such as NFKB2 and ICAM1. Exhibits opposing effects to IGFBP2 on cell invasion.1 Publication

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Migration and invasion-inhibitory protein
Alternative name(s):
IGFBP2-binding protein
Invasion-inhibitory protein 45
Short name:
IIp45
Gene namesi
Name:MIIP
Synonyms:IIP45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25715. MIIP.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165751598.

Polymorphism and mutation databases

BioMutaiMIIP.
DMDMi296439302.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Migration and invasion-inhibitory proteinPRO_0000324322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei303 – 3031PhosphoserineCombined sources

Post-translational modificationi

Isoform 2 is degraded by the ubiquitin-proteasome pathway.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5JXC2.
MaxQBiQ5JXC2.
PaxDbiQ5JXC2.
PRIDEiQ5JXC2.

PTM databases

iPTMnetiQ5JXC2.
PhosphoSiteiQ5JXC2.

Expressioni

Tissue specificityi

Ubiquitous. Isoform 1 is expressed in brain but underexpressed in glioma tissues, at protein level. Isoform 2 is not detected in normal organs, but is expressed in gliomas with increasing levels with glioma progression. On the contrary, at protein level, isoform 2 is not detected in gliomas, suggesting that this isoform is unstable in glioma cells.1 Publication

Inductioni

Up-regulated by IGFBP2.1 Publication

Gene expression databases

BgeeiQ5JXC2.
GenevisibleiQ5JXC2. HS.

Organism-specific databases

HPAiHPA044948.

Interactioni

Subunit structurei

Interacts with IGFBP2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
STK16O757163EBI-2801965,EBI-749295

Protein-protein interaction databases

BioGridi121947. 8 interactions.
IntActiQ5JXC2. 8 interactions.
STRINGi9606.ENSP00000235332.

Structurei

3D structure databases

ProteinModelPortaliQ5JXC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni239 – 28244Interaction with IGFBP2Add
BLAST

Phylogenomic databases

eggNOGiENOG410II0C. Eukaryota.
ENOG4111PRQ. LUCA.
GeneTreeiENSGT00390000003768.
HOGENOMiHOG000095519.
InParanoidiQ5JXC2.
OMAiTPCRLCR.
OrthoDBiEOG7SV0WS.
PhylomeDBiQ5JXC2.
TreeFamiTF335829.

Family and domain databases

InterProiIPR031466. MIIP.
[Graphical view]
PfamiPF15734. MIIP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5JXC2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEAEELAQL RLLNLELLRQ LWVGQDAVRR SVARAASESS LESSSSYNSE
60 70 80 90 100
TPSTPETSST SLSTSCPRGR SSVWGPPDAC RGDLRDVARS GVASLPPAKC
110 120 130 140 150
QHQESLGRPR PHSAPSLGTS SLRDPEPSGR LGDPGPQEAQ TPRSILAQQS
160 170 180 190 200
KLSKPRVTFS EESAVPKRSW RLRPYLGYDW IAGSLDTSSS ITSQPEAFFS
210 220 230 240 250
KLQEFRETNK EECICSHPEP QLPGLRESSG SGVEEDHECV YCYRVNRRLF
260 270 280 290 300
PVPVDPGTPC RLCRTPRDQQ GPGTLAQPAH VRVSIPLSIL EPPHRYHIHR
310 320 330 340 350
RKSFDASDTL ALPRHCLLGW DIFPPKSEKS SAPRNLDLWS SVSAEAQHQK
360 370 380
LSGTSSPFHP ASPMQMLPPT PTWSVPQVPR PHVPRQKP
Length:388
Mass (Da):42,824
Last modified:May 18, 2010 - v3
Checksum:i31854F9B1669B00C
GO
Isoform 2 (identifier: Q5JXC2-2) [UniParc]FASTAAdd to basket

Also known as: IIP45S

The sequence of this isoform differs from the canonical sequence as follows:
     282-388: RVSIPLSILE...PRPHVPRQKP → STACWAGTFF...PTSHGRSPED

Show »
Length:359
Mass (Da):39,292
Checksum:i68EB9BA04ECACF33
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831G → V in BAB14062 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti99 – 991K → N.3 Publications
Corresponds to variant rs11553925 [ dbSNP | Ensembl ].
VAR_039729
Natural varianti142 – 1421P → S.1 Publication
Corresponds to variant rs11588712 [ dbSNP | Ensembl ].
VAR_039730
Natural varianti167 – 1671K → E.4 Publications
Corresponds to variant rs2295283 [ dbSNP | Ensembl ].
VAR_039731
Natural varianti247 – 2471R → W.
Corresponds to variant rs2295289 [ dbSNP | Ensembl ].
VAR_059688
Natural varianti288 – 2881S → L.
Corresponds to variant rs35317667 [ dbSNP | Ensembl ].
VAR_059689
Natural varianti381 – 3811P → S.
Corresponds to variant rs34874602 [ dbSNP | Ensembl ].
VAR_059690

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei282 – 388107RVSIP…PRQKP → STACWAGTFFLRSLRKAQPP GTWTSGPLYPLRPSTRSCPA PAQALFTRPHQCRCCPRPRP GQCPRSLGPTSHGRSPED in isoform 2. 1 PublicationVSP_032214Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ618905 mRNA. Translation: ACM90320.1.
AK022500 mRNA. Translation: BAB14062.1.
AK024020 mRNA. Translation: BAB14781.1.
AL096840 Genomic DNA. Translation: CAI19089.1.
CH471130 Genomic DNA. Translation: EAW71729.1.
BC008068 mRNA. Translation: AAH08068.1.
CCDSiCCDS143.1. [Q5JXC2-1]
RefSeqiNP_068752.2. NM_021933.3. [Q5JXC2-1]
XP_011540197.1. XM_011541895.1. [Q5JXC2-1]
XP_011540198.1. XM_011541896.1. [Q5JXC2-1]
UniGeneiHs.8595.

Genome annotation databases

EnsembliENST00000235332; ENSP00000235332; ENSG00000116691. [Q5JXC2-1]
GeneIDi60672.
KEGGihsa:60672.
UCSCiuc001ato.3. human. [Q5JXC2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ618905 mRNA. Translation: ACM90320.1.
AK022500 mRNA. Translation: BAB14062.1.
AK024020 mRNA. Translation: BAB14781.1.
AL096840 Genomic DNA. Translation: CAI19089.1.
CH471130 Genomic DNA. Translation: EAW71729.1.
BC008068 mRNA. Translation: AAH08068.1.
CCDSiCCDS143.1. [Q5JXC2-1]
RefSeqiNP_068752.2. NM_021933.3. [Q5JXC2-1]
XP_011540197.1. XM_011541895.1. [Q5JXC2-1]
XP_011540198.1. XM_011541896.1. [Q5JXC2-1]
UniGeneiHs.8595.

3D structure databases

ProteinModelPortaliQ5JXC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121947. 8 interactions.
IntActiQ5JXC2. 8 interactions.
STRINGi9606.ENSP00000235332.

PTM databases

iPTMnetiQ5JXC2.
PhosphoSiteiQ5JXC2.

Polymorphism and mutation databases

BioMutaiMIIP.
DMDMi296439302.

Proteomic databases

EPDiQ5JXC2.
MaxQBiQ5JXC2.
PaxDbiQ5JXC2.
PRIDEiQ5JXC2.

Protocols and materials databases

DNASUi60672.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000235332; ENSP00000235332; ENSG00000116691. [Q5JXC2-1]
GeneIDi60672.
KEGGihsa:60672.
UCSCiuc001ato.3. human. [Q5JXC2-1]

Organism-specific databases

CTDi60672.
GeneCardsiMIIP.
H-InvDBHIX0000137.
HGNCiHGNC:25715. MIIP.
HPAiHPA044948.
MIMi608772. gene.
neXtProtiNX_Q5JXC2.
PharmGKBiPA165751598.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410II0C. Eukaryota.
ENOG4111PRQ. LUCA.
GeneTreeiENSGT00390000003768.
HOGENOMiHOG000095519.
InParanoidiQ5JXC2.
OMAiTPCRLCR.
OrthoDBiEOG7SV0WS.
PhylomeDBiQ5JXC2.
TreeFamiTF335829.

Miscellaneous databases

GenomeRNAii60672.
NextBioi65469.
PROiQ5JXC2.
SOURCEiSearch...

Gene expression databases

BgeeiQ5JXC2.
GenevisibleiQ5JXC2. HS.

Family and domain databases

InterProiIPR031466. MIIP.
[Graphical view]
PfamiPF15734. MIIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of the invasion inhibitory gene IIp45 by alternative splicing in gliomas."
    Song S.W., Fuller G.N., Zheng H., Zhang W.
    Cancer Res. 65:3562-3567(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS ASN-99; SER-142 AND GLU-167.
  2. "Molecular cloning and bioinformatic analysis of IIP45 gene."
    Liu S., Wu Y., Zhang W.
    Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-99 AND GLU-167.
  4. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-167.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASN-99 AND GLU-167.
    Tissue: Skin.
  7. "IIp45, an insulin-like growth factor binding protein 2 (IGFBP-2) binding protein, antagonizes IGFBP-2 stimulation of glioma cell invasion."
    Song S.W., Fuller G.N., Khan A., Kong S., Shen W., Taylor E., Ramdas L., Lang F.F., Zhang W.
    Proc. Natl. Acad. Sci. U.S.A. 100:13970-13975(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGION, TISSUE SPECIFICITY, INTERACTION WITH IGFBP2.
  8. "IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T."
    Frommer K.W., Reichenmiller K., Schutt B.S., Hoeflich A., Ranke M.B., Dodt G., Elmlinger M.W.
    J. Mol. Endocrinol. 37:13-23(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY IGFBP2.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMIIP_HUMAN
AccessioniPrimary (citable) accession number: Q5JXC2
Secondary accession number(s): C0KL22
, Q96HU6, Q9H839, Q9HA00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 18, 2010
Last modified: April 13, 2016
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.