Q5JVS0 (HABP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intracellular hyaluronan-binding protein 4
Short name=IHABP-4
Short name=IHABP4

Alternative name(s):
Ki-1/57 intracellular antigen

Gene names

Name:

HABP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in nuclear functions such as the remodeling of chromatin and the regulation of transcription. Ref.6 Ref.7
Subunit structure	Interacts with the C-terminus of CHD3. Interacts via its C-terminal region with GNB2L1. Interacts with p53/TP53. Ref.5 Ref.6 Ref.7 UniProtKB Q9JKS5
Subcellular location	Cytoplasm. Nucleus. Note: Transported into the nuclear compartment in activated leukocytes. Ref.4 Ref.6 Ref.7
Tissue specificity	Highly expressed in brain, heart, and kidney, and moderately expressed in skeletal muscle. Also expressed in a variety of tumor cell lines and in activated but not resting leukocytes. Ref.4 Ref.5
Post-translational modification	Phosphorylated by phorbol 12-myristate 13-acetate (PMA)-activated PKC isoforms at Thr-354 and Thr-375 Probable. Ref.6 Ref.8
Miscellaneous	Able to bind hyaluronan. However, its intracellular localization suggests that this interaction may not be relevant in vivo. The interaction with GNB2L1 is abolished upon activation of L540 tumor cells with PMA, which results in phosphorylation and exit of HABP4 from the nucleus. Ref.6

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to mechanical stimulus Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of DNA binding Inferred from direct assay. Source: UniProtKB platelet activation Traceable author statement. Source: Reactome platelet degranulation Traceable author statement. Source: Reactome regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome nucleus Inferred from sequence or structural similarity. Source: UniProtKB sarcomere Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRMT1	Q99873	2	EBI-523625,EBI-78738

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.4 (identifier: Q5JVS0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q5JVS0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     172-276: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Intracellular hyaluronan-binding protein 4		PRO_0000257972
Regions
	Coiled coil	40 – 64	25	Potential
Amino acid modifications
	Modified residue	97	1	Phosphoserine Ref.8
	Modified residue	108	1	Phosphoserine Ref.8
	Modified residue	354	1	Phosphothreonine; by PKC Probable Ref.6
	Modified residue	375	1	Phosphothreonine; by PKC Probable Ref.6
Natural variations
	Alternative sequence	172 – 276	105	Missing in isoform 2. Ref.3		VSP_052194
Experimental info
	Sequence conflict	104	1	Q → H in AAF62546. Ref.1
	Sequence conflict	115	1	A → S in AAC31117. Ref.4
	Sequence conflict	388 – 389	2	YG → NY in AAF62546. Ref.1
	Sequence conflict	388 – 389	2	YG → NY in AAC31117. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 98749EEC5CB92155	FASTA	413	45,785
	10 20 30 40 50 60 MKGALGSPVA AAGAAMQESF GCVVANRFHQ LLDDESDPFD ILREAERRRQ QQLQRKRRDE 70 80 90 100 110 120 AAAAAGAGPR GGRSPAGASG HRAGAGGRRE SQKERKSLPA PVAQRPDSPG GGLQAPGQKR 130 140 150 160 170 180 TPRRGEQQGW NDSRGPEGML ERAERRSYRE YRPYETERQA DFTAEKFPDE KPGDRFDRDR 190 200 210 220 230 240 PLRGRGGPRG GMRGRGRGGP GNRVFDAFDQ RGKREFERYG GNDKIAVRTE DNMGGCGVRT 250 260 270 280 290 300 WGSGKDTSDV EPTAPMEEPT VVEESQGTPE EESPAKVPEL EVEEETQVQE MTLDEWKNLQ 310 320 330 340 350 360 EQTRPKPEFN IRKPESTVPS KAVVIHKSKY RDDMVKDDYE DDSHVFRKPA NDITSQLEIN 370 380 390 400 410 FGNLPRPGRG ARGGTRGGRG RIRRAENYGP RAEVVMQDVA PNPDDPEDFP ALS
	Isoform 2 [UniParc]. Checksum: CFA9C86A34BF5514	FASTA	308	34,365
	10 20 30 40 50 60 MKGALGSPVA AAGAAMQESF GCVVANRFHQ LLDDESDPFD ILREAERRRQ QQLQRKRRDE 70 80 90 100 110 120 AAAAAGAGPR GGRSPAGASG HRAGAGGRRE SQKERKSLPA PVAQRPDSPG GGLQAPGQKR 130 140 150 160 170 180 TPRRGEQQGW NDSRGPEGML ERAERRSYRE YRPYETERQA DFTAEKFPDE KVPELEVEEE 190 200 210 220 230 240 TQVQEMTLDE WKNLQEQTRP KPEFNIRKPE STVPSKAVVI HKSKYRDDMV KDDYEDDSHV 250 260 270 280 290 300 FRKPANDITS QLEINFGNLP RPGRGARGGT RGGRGRIRRA ENYGPRAEVV MQDVAPNPDD PEDFPALS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a novel intracellular hyaluronan-binding protein." Huang L., Grammatikakis N., Yoneda M., Banerjee S.D., Toole B.P. J. Biol. Chem. 275:29829-29839(2000) [PubMed: 10887182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Characterization, mapping and partial cDNA sequence of the 57-kD intracellular Ki-1 antigen." Kobarg J., Schnittger S., Fonatsch C., Lemke H., Bowen M.A., Buck F., Hansen H.P. Exp. Clin. Immunogenet. 14:273-280(1997) [PubMed: 9523163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-413 (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3." Lemos T.A., Passos D.O., Nery F.C., Kobarg J. FEBS Lett. 533:14-20(2003) [PubMed: 12505151] [Abstract] Cited for: INTERACTION WITH CHD3, TISSUE SPECIFICITY.
[6]	"Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C." Nery F.C., Passos D.O., Garcia V.S., Kobarg J. J. Biol. Chem. 279:11444-11455(2004) [PubMed: 14699138] [Abstract] Cited for: FUNCTION, INTERACTION WITH GNB2L1, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[7]	"Evidence for the interaction of the regulatory protein Ki-1/57 with p53 and its interacting proteins." Nery F.C., Rui E., Kuniyoshi T.M., Kobarg J. Biochem. Biophys. Res. Commun. 341:847-855(2006) [PubMed: 16455055] [Abstract] Cited for: FUNCTION, INTERACTION WITH TP53, SUBCELLULAR LOCATION.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND SER-108, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF241831 mRNA. Translation: AAF62546.1. AL133477 Genomic DNA. Translation: CAI39620.1. AL133477 Genomic DNA. Translation: CAI39621.1. BC018788 mRNA. Translation: AAH18788.1. U77327 mRNA. Translation: AAC31117.1.
IPI	IPI00018583. IPI00103004.
RefSeq	NP_055097.2. NM_014282.2.
UniGene	Hs.494567.
3D structure databases
ProteinModelPortal	Q5JVS0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5JVS0. 5 interactions.
MINT	MINT-1346616.
STRING	Q5JVS0.
PTM databases
PhosphoSite	Q5JVS0.
Polymorphism databases
DMDM	74742472.
Proteomic databases
PRIDE	Q5JVS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375249; ENSP00000364398; ENSG00000130956.
GeneID	22927.
KEGG	hsa:22927.
NMPDR	fig|9606.3.peg.31600.
UCSC	uc010msg.1. human. uc010msh.1. human.
Organism-specific databases
CTD	22927.
GeneCards	GC09P099212.
H-InvDB	HIX0008206.
HGNC	HGNC:17062. HABP4.
neXtProt	NX_Q5JVS0.
PharmGKB	PA38434.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05710.
GeneTree	ENSGT00520000055591.
HOGENOM	HBG713968.
HOVERGEN	HBG056357.
InParanoid	Q5JVS0.
OMA	RTEDNMG.
PhylomeDB	Q5JVS0.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q5JVS0.
Bgee	Q5JVS0.
CleanEx	HS_HABP4.
Genevestigator	Q5JVS0.
GermOnline	ENSG00000130956. Homo sapiens.
Family and domain databases
InterPro	IPR006861. HABP4_PAIRBP1-bd. [Graphical view]
Pfam	PF04774. HABP4_PAI-RBP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	43643.

Entry information

Entry name

HABP4_HUMAN

Accession

Primary (citable) accession number: Q5JVS0
Secondary accession number(s): O75804, Q8WV33, Q9NYJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.