Q5JUK3 (KCNT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily T member 1

Alternative name(s):
KCa4.1

Gene names

Name:	KCNT1
Synonyms:	KIAA1422

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1230 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) By similarity.
Subunit structure	Interacts with CRBN via its cytoplasmic C-terminus By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. Ref.3
Post-translational modification	Phosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity By similarity.
Sequence similarities	Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily. [View classification] Contains 1 RCK N-terminal domain.
Sequence caution	The sequence BAA92660.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI39240.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Calcium Potassium
Molecular function	Ion channel Potassium channel
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	voltage-gated potassium channel complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	calcium-activated potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome nucleotide binding Inferred from electronic annotation. Source: InterPro voltage-gated potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5JUK3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q5JUK3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: MARAKLPRSP...DFSLDSSLSQ → MPLPDGARTP...GDPSFQNDDR
     1033-1033: E → EPHDLRAQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5JUK3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: MARAKLPRSP...DFSLDSSLSQ → MPLPDGARTP...GDPSFQNDDR
     1033-1033: E → EPHDLRAQ
     1142-1162: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1230	1230	Potassium channel subfamily T member 1		PRO_0000054090
Regions
	Topological domain	1 – 97	97	Cytoplasmic Potential
	Transmembrane	98 – 118	21	Helical; Name=Segment S1; Potential
	Topological domain	119 – 155	37	Extracellular Potential
	Transmembrane	156 – 176	21	Helical; Name=Segment S2; Potential
	Topological domain	177 – 187	11	Cytoplasmic Potential
	Transmembrane	188 – 208	21	Helical; Name=Segment S3; Potential
	Topological domain	209 – 213	5	Extracellular Potential
	Transmembrane	214 – 226	13	Helical; Name=Segment S4; Potential
	Topological domain	227 – 251	25	Cytoplasmic Potential
	Transmembrane	252 – 272	21	Helical; Name=Segment S5; Potential
	Topological domain	273 – 281	9	Extracellular Potential
	Intramembrane	282 – 302	21	Pore-forming; Potential
	Topological domain	303 – 304	2	Extracellular Potential
	Transmembrane	305 – 325	21	Helical; Name=Segment S6; Potential
	Topological domain	326 – 1230	905	Cytoplasmic Potential
	Domain	475 – 596	122	RCK N-terminal
Amino acid modifications
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	137	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 66	66	MARAK…SSLSQ → MPLPDGARTPGGVCREARGG GYTNRTFEFDDGQCAPRRPC AGDGALLDTAGFKMSDLDSE VLPLPPRYRFRDLLLGDPSF QNDDR in isoform 2 and isoform 3.		VSP_015470
	Alternative sequence	1033	1	E → EPHDLRAQ in isoform 2 and isoform 3.		VSP_015471
	Alternative sequence	1142 – 1162	21	Missing in isoform 3.		VSP_044476
Experimental info
	Sequence conflict	615	1	A → V in AAI71770. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 482D70015434493E	FASTA	1,230	138,343
	10 20 30 40 50 60 MARAKLPRSP SEGKAGPGGA PAGAAAPEEP HGLSPLLPAR GGGSVGSDVG QRLPVEDFSL 70 80 90 100 110 120 DSSLSQVQVE FYVNENTFKE RLKLFFIKNQ RSSLRIRLFN FSLKLLTCLL YIVRVLLDDP 130 140 150 160 170 180 ALGIGCWGCP KQNYSFNDSS SEINWAPILW VERKMTLWAI QVIVAIISFL ETMLLIYLSY 190 200 210 220 230 240 KGNIWEQIFR VSFVLEMINT LPFIITIFWP PLRNLFIPVF LNCWLAKHAL ENMINDFHRA 250 260 270 280 290 300 ILRTQSAMFN QVLILFCTLL CLVFTGTCGI QHLERAGENL SLLTSFYFCI VTFSTVGYGD 310 320 330 340 350 360 VTPKIWPSQL LVVIMICVAL VVLPLQFEEL VYLWMERQKS GGNYSRHRAQ TEKHVVLCVS 370 380 390 400 410 420 SLKIDLLMDF LNEFYAHPRL QDYYVVILCP TEMDVQVRRV LQIPLWSQRV IYLQGSALKD 430 440 450 460 470 480 QDLMRAKMDN GEACFILSSR NEVDRTAADH QTILRAWAVK DFAPNCPLYV QILKPENKFH 490 500 510 520 530 540 VKFADHVVCE EECKYAMLAL NCICPATSTL ITLLVHTSRG QEGQESPEQW QRMYGRCSGN 550 560 570 580 590 600 EVYHIRMGDS KFFREYEGKS FTYAAFHAHK KYGVCLIGLK REDNKSILLN PGPRHILAAS 610 620 630 640 650 660 DTCFYINITK EENSAFIFKQ EEKRKKRAFS GQGLHEGPAR LPVHSIIASM GTVAMDLQGT 670 680 690 700 710 720 EHRPTQSGGG GGGSKLALPT ENGSGSRRPS IAPVLELADS SALLPCDLLS DQSEDEVTPS 730 740 750 760 770 780 DDEGLSVVEY VKGYPPNSPY IGSSPTLCHL LPVKAPFCCL RLDKGCKHNS YEDAKAYGFK 790 800 810 820 830 840 NKLIIVSAET AGNGLYNFIV PLRAYYRSRK ELNPIVLLLD NKPDHHFLEA ICCFPMVYYM 850 860 870 880 890 900 EGSVDNLDSL LQCGIIYADN LVVVDKESTM SAEEDYMADA KTIVNVQTMF RLFPSLSITT 910 920 930 940 950 960 ELTHPSNMRF MQFRAKDSYS LALSKLEKRE RENGSNLAFM FRLPFAAGRV FSISMLDTLL 970 980 990 1000 1010 1020 YQSFVKDYMI TITRLLLGLD TTPGSGYLCA MKITEGDLWI RTYGRLFQKL CSSSAEIPIG 1030 1040 1050 1060 1070 1080 IYRTESHVFS TSESQISVNV EDCEDTREVK GPWGSRAGTG GSSQGRHTGG GDPAEHPLLR 1090 1100 1110 1120 1130 1140 RKSLQWARRL SRKAPKQAGR AAAAEWISQQ RLSLYRRSER QELSELVKNR MKHLGLPTTG 1150 1160 1170 1180 1190 1200 YEDVANLTAS DVMNRVNLGY LQDEMNDHQN TLSYVLINPP PDTRLEPSDI VYLIRSDPLA 1210 1220 1230 HVASSSQSRK SSCSHKLSSC NPETRDETQL
	Isoform 2 [UniParc]. Checksum: D6F9FCC8D1383EE6	FASTA	1,256	142,004
	10 20 30 40 50 60 MPLPDGARTP GGVCREARGG GYTNRTFEFD DGQCAPRRPC AGDGALLDTA GFKMSDLDSE 70 80 90 100 110 120 VLPLPPRYRF RDLLLGDPSF QNDDRVQVEF YVNENTFKER LKLFFIKNQR SSLRIRLFNF 130 140 150 160 170 180 SLKLLTCLLY IVRVLLDDPA LGIGCWGCPK QNYSFNDSSS EINWAPILWV ERKMTLWAIQ 190 200 210 220 230 240 VIVAIISFLE TMLLIYLSYK GNIWEQIFRV SFVLEMINTL PFIITIFWPP LRNLFIPVFL 250 260 270 280 290 300 NCWLAKHALE NMINDFHRAI LRTQSAMFNQ VLILFCTLLC LVFTGTCGIQ HLERAGENLS 310 320 330 340 350 360 LLTSFYFCIV TFSTVGYGDV TPKIWPSQLL VVIMICVALV VLPLQFEELV YLWMERQKSG 370 380 390 400 410 420 GNYSRHRAQT EKHVVLCVSS LKIDLLMDFL NEFYAHPRLQ DYYVVILCPT EMDVQVRRVL 430 440 450 460 470 480 QIPLWSQRVI YLQGSALKDQ DLMRAKMDNG EACFILSSRN EVDRTAADHQ TILRAWAVKD 490 500 510 520 530 540 FAPNCPLYVQ ILKPENKFHV KFADHVVCEE ECKYAMLALN CICPATSTLI TLLVHTSRGQ 550 560 570 580 590 600 EGQESPEQWQ RMYGRCSGNE VYHIRMGDSK FFREYEGKSF TYAAFHAHKK YGVCLIGLKR 610 620 630 640 650 660 EDNKSILLNP GPRHILAASD TCFYINITKE ENSAFIFKQE EKRKKRAFSG QGLHEGPARL 670 680 690 700 710 720 PVHSIIASMG TVAMDLQGTE HRPTQSGGGG GGSKLALPTE NGSGSRRPSI APVLELADSS 730 740 750 760 770 780 ALLPCDLLSD QSEDEVTPSD DEGLSVVEYV KGYPPNSPYI GSSPTLCHLL PVKAPFCCLR 790 800 810 820 830 840 LDKGCKHNSY EDAKAYGFKN KLIIVSAETA GNGLYNFIVP LRAYYRSRKE LNPIVLLLDN 850 860 870 880 890 900 KPDHHFLEAI CCFPMVYYME GSVDNLDSLL QCGIIYADNL VVVDKESTMS AEEDYMADAK 910 920 930 940 950 960 TIVNVQTMFR LFPSLSITTE LTHPSNMRFM QFRAKDSYSL ALSKLEKRER ENGSNLAFMF 970 980 990 1000 1010 1020 RLPFAAGRVF SISMLDTLLY QSFVKDYMIT ITRLLLGLDT TPGSGYLCAM KITEGDLWIR 1030 1040 1050 1060 1070 1080 TYGRLFQKLC SSSAEIPIGI YRTESHVFST SEPHDLRAQS QISVNVEDCE DTREVKGPWG 1090 1100 1110 1120 1130 1140 SRAGTGGSSQ GRHTGGGDPA EHPLLRRKSL QWARRLSRKA PKQAGRAAAA EWISQQRLSL 1150 1160 1170 1180 1190 1200 YRRSERQELS ELVKNRMKHL GLPTTGYEDV ANLTASDVMN RVNLGYLQDE MNDHQNTLSY 1210 1220 1230 1240 1250 VLINPPPDTR LEPSDIVYLI RSDPLAHVAS SSQSRKSSCS HKLSSCNPET RDETQL
	Isoform 3 [UniParc]. Checksum: AACACE2A0A39A693	FASTA	1,235	139,700
	10 20 30 40 50 60 MPLPDGARTP GGVCREARGG GYTNRTFEFD DGQCAPRRPC AGDGALLDTA GFKMSDLDSE 70 80 90 100 110 120 VLPLPPRYRF RDLLLGDPSF QNDDRVQVEF YVNENTFKER LKLFFIKNQR SSLRIRLFNF 130 140 150 160 170 180 SLKLLTCLLY IVRVLLDDPA LGIGCWGCPK QNYSFNDSSS EINWAPILWV ERKMTLWAIQ 190 200 210 220 230 240 VIVAIISFLE TMLLIYLSYK GNIWEQIFRV SFVLEMINTL PFIITIFWPP LRNLFIPVFL 250 260 270 280 290 300 NCWLAKHALE NMINDFHRAI LRTQSAMFNQ VLILFCTLLC LVFTGTCGIQ HLERAGENLS 310 320 330 340 350 360 LLTSFYFCIV TFSTVGYGDV TPKIWPSQLL VVIMICVALV VLPLQFEELV YLWMERQKSG 370 380 390 400 410 420 GNYSRHRAQT EKHVVLCVSS LKIDLLMDFL NEFYAHPRLQ DYYVVILCPT EMDVQVRRVL 430 440 450 460 470 480 QIPLWSQRVI YLQGSALKDQ DLMRAKMDNG EACFILSSRN EVDRTAADHQ TILRAWAVKD 490 500 510 520 530 540 FAPNCPLYVQ ILKPENKFHV KFADHVVCEE ECKYAMLALN CICPATSTLI TLLVHTSRGQ 550 560 570 580 590 600 EGQESPEQWQ RMYGRCSGNE VYHIRMGDSK FFREYEGKSF TYAAFHAHKK YGVCLIGLKR 610 620 630 640 650 660 EDNKSILLNP GPRHILAASD TCFYINITKE ENSAFIFKQE EKRKKRAFSG QGLHEGPARL 670 680 690 700 710 720 PVHSIIASMG TVAMDLQGTE HRPTQSGGGG GGSKLALPTE NGSGSRRPSI APVLELADSS 730 740 750 760 770 780 ALLPCDLLSD QSEDEVTPSD DEGLSVVEYV KGYPPNSPYI GSSPTLCHLL PVKAPFCCLR 790 800 810 820 830 840 LDKGCKHNSY EDAKAYGFKN KLIIVSAETA GNGLYNFIVP LRAYYRSRKE LNPIVLLLDN 850 860 870 880 890 900 KPDHHFLEAI CCFPMVYYME GSVDNLDSLL QCGIIYADNL VVVDKESTMS AEEDYMADAK 910 920 930 940 950 960 TIVNVQTMFR LFPSLSITTE LTHPSNMRFM QFRAKDSYSL ALSKLEKRER ENGSNLAFMF 970 980 990 1000 1010 1020 RLPFAAGRVF SISMLDTLLY QSFVKDYMIT ITRLLLGLDT TPGSGYLCAM KITEGDLWIR 1030 1040 1050 1060 1070 1080 TYGRLFQKLC SSSAEIPIGI YRTESHVFST SEPHDLRAQS QISVNVEDCE DTREVKGPWG 1090 1100 1110 1120 1130 1140 SRAGTGGSSQ GRHTGGGDPA EHPLLRRKSL QWARRLSRKA PKQAGRAAAA EWISQQRLSL 1150 1160 1170 1180 1190 1200 YRRSERQELS ELVKNRMKHL GLPTTGYDEM NDHQNTLSYV LINPPPDTRL EPSDIVYLIR 1210 1220 1230 SDPLAHVASS SQSRKSSCSH KLSSCNPETR DETQL

References

[1]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1103 (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL158822 Genomic DNA. Translation: CAI39240.1. Different initiation. BC136618 mRNA. Translation: AAI36619.1. BC171770 mRNA. Translation: AAI71770.1. AB037843 mRNA. Translation: BAA92660.1. Different initiation.
IPI	IPI00143205. IPI00945462. IPI00946359.
RefSeq	NP_065873.2. NM_020822.2.
UniGene	Hs.104950.
3D structure databases
ProteinModelPortal	Q5JUK3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000298480.
PTM databases
PhosphoSite	Q5JUK3.
Polymorphism databases
DMDM	73920089.
Proteomic databases
PaxDb	Q5JUK3.
PRIDE	Q5JUK3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263604; ENSP00000263604; ENSG00000107147. ENST00000298480; ENSP00000298480; ENSG00000107147. ENST00000371757; ENSP00000360822; ENSG00000107147.
GeneID	57582.
KEGG	hsa:57582.
Organism-specific databases
CTD	57582.
GeneCards	GC09P138594.
HGNC	HGNC:18865. KCNT1.
MIM	608167. gene.
neXtProt	NX_Q5JUK3.
PharmGKB	PA38725.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1226.
HOGENOM	HOG000231460.
HOVERGEN	HBG055190.
InParanoid	Q5JUK3.
KO	K04946.
OMA	IRREENK.
Gene expression databases
ArrayExpress	Q5JUK3.
Bgee	Q5JUK3.
CleanEx	HS_KCNT1.
Genevestigator	Q5JUK3.
GermOnline	ENSG00000107147. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013099. Ion_trans_2. IPR003929. K_chnl_Ca-activ_BK_asu. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF03493. BK_channel_a. 1 hit. PF07885. Ion_trans_2. 1 hit. [Graphical view]
PROSITE	PS51201. RCK_N. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57582.
NextBio	64138.
SOURCE	Search...

Entry information

Entry name

KCNT1_HUMAN

Accession

Primary (citable) accession number: Q5JUK3
Secondary accession number(s): B7ZVY4, B9EGP2, Q9P2C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.