Q5JS13 (RGPS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-specific guanine nucleotide-releasing factor RalGPS1

Alternative name(s):
Ral GEF with PH domain and SH3-binding motif 1
Ral guanine nucleotide exchange factor 2
Short name=RalGEF 2
RalA exchange factor RalGPS1

Gene names

Name:	RALGPS1
Synonyms:	KIAA0351, RALGEF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide exchange factor (GEF) for the small GTPase RALA. May be involved in cytoskeletal organization By similarity. Guanine nucleotide exchange factor for. Ref.1 Ref.7
Subunit structure	Interacts with the SH3 domains of GRB2, NCK1, PLCG1 and SRC. Ref.1
Subcellular location	Cytoplasm. Cell membrane. Note: Associates with membranes through the PH domain. Ref.7
Tissue specificity	Widely expressed (at protein level). Isoform 2 is expressed in brain, colon, kidney, pancreas, prostate, skeletal muscle, small intestine, testis, thymus and uterus. Isoform 1 is expressed at high levels in heart and testis and at lower levels in brain, pancreas, skeletal muscle, small intestine and thymus. Ref.1 Ref.7
Domain	The PH domain mediates binding to membranes. It is required for efficient GEF activity.
Sequence similarities	Contains 1 PH domain. Contains 1 Ras-GEF domain.
Sequence caution	The sequence AAH19329.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA20808.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Guanine-nucleotide releasing factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Non-traceable author statement Ref.1. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanyl-nucleotide exchange factor activity Non-traceable author statement Ref.1. Source: UniProtKB phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5JS13-1)

Also known as: RALGPS1B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5JS13-2)

Also known as: RALGPS1A;

The sequence of this isoform differs from the canonical sequence as follows:
     347-415: MMCQLSVVES...FSEEMSSGLE → RGRLYATLGPNWRVPVRNSPRTRSCVY
     483-490: Missing.
     549-557: VPANLMSFE → EAGAAPGPTGTDSHEVDHLEGGAGKEAGPCA

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5JS13-3)

The sequence of this isoform differs from the canonical sequence as follows:
     304-384: GPSAGSGSAR...LESRSPRRGL → VSHLSSLSHQ...TLGSRSRRIT
     385-557: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q5JS13-4)

The sequence of this isoform differs from the canonical sequence as follows:
     304-305: GP → AM
     306-557: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q5JS13-5)

The sequence of this isoform differs from the canonical sequence as follows:
     205-213: IYLLDLIYI → RSLNWLIFF
     214-557: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.

Isoform 6 (identifier: Q5JS13-6)

The sequence of this isoform differs from the canonical sequence as follows:
     73-108: ELASCGWSKKEKHSLAPNVVAFTRRFNQVSFWVVRE → KGWPARMEDSLQKSTVSSRPHDTLQFRKTLPVGPGT
     109-557: Missing.

Note: No experimental confirmation available.

Isoform 7 (identifier: Q5JS13-7)

The sequence of this isoform differs from the canonical sequence as follows:
     347-415: MMCQLSVVES...FSEEMSSGLE → RGRLYATLGPNWRVPVRNSPRTRSCVY
     549-557: VPANLMSFE → EAGAAPGPTGTDSHEVDHLEGGAGKEAGPCA

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 557

557

Ras-specific guanine nucleotide-releasing factor RalGPS1

PRO_0000333192

Regions

Domain

50 – 289

240

Ras-GEF

Domain

431 – 543

113

PH

Region

433 – 557

125

Required for stimulation of nucleotide exchange by RALA

Motif

330 – 333

4

PXXP

Compositional bias

21 – 26

6

Poly-Ser

Natural variations

Alternative sequence

73 – 108

36

ELASC…WVVRE → KGWPARMEDSLQKSTVSSRP HDTLQFRKTLPVGPGT in isoform 6.

VSP_033471

Alternative sequence

109 – 557

449

Missing in isoform 6.

VSP_033472

Alternative sequence

205 – 213

9

IYLLDLIYI → RSLNWLIFF in isoform 5.

VSP_033473

Alternative sequence

214 – 557

344

Missing in isoform 5.

VSP_033474

Alternative sequence

304 – 384

81

GPSAG…PRRGL → VSHLSSLSHQGQAEEARLKP TSGQHPAWMWPSSSRVPAAP PASAAPRSPWPRNLRNDQGQ PGAVALTCNPSTLGSRSRRI T in isoform 3.

VSP_033475

Alternative sequence

304 – 305

2

GP → AM in isoform 4.

VSP_033476

Alternative sequence

306 – 557

252

Missing in isoform 4.

VSP_033477

Alternative sequence

347 – 415

69

MMCQL…SSGLE → RGRLYATLGPNWRVPVRNSP RTRSCVY in isoform 2 and isoform 7.

VSP_033478

Alternative sequence

385 – 557

173

Missing in isoform 3.

VSP_033479

Alternative sequence

483 – 490

8

Missing in isoform 2.

VSP_033480

Alternative sequence

549 – 557

9

VPANLMSFE → EAGAAPGPTGTDSHEVDHLE GGAGKEAGPCA in isoform 2 and isoform 7.

VSP_033481

Natural variant

290

1

S → N.
Corresponds to variant rs55918931 [ dbSNP | Ensembl ].

VAR_061785

Experimental info

Mutagenesis

148

1

L → A: Loss of activity. Ref.7

Sequence conflict

86

1

S → T in AAH32372. Ref.6

Sequence conflict

189

1

E → G in BAG61198. Ref.3

Sequence conflict

251

1

H → R in BAG61198. Ref.3

Secondary structure

1

.

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557

Helix Strand Turn

Details...

Helix

46 – 48

3

Helix

51 – 67

17

Helix

71 – 76

6

Helix

84 – 87

4

Helix

89 – 110

22

Helix

115 – 134

20

Helix

138 – 148

11

Helix

151 – 154

4

Helix

157 – 161

5

Helix

165 – 177

13

Helix

181 – 192

12

Turn

193 – 196

4

Helix

203 – 216

14

Beta strand

219 – 221

3

Helix

223 – 245

23

Helix

256 – 263

8

Helix

269 – 271

3

Helix

272 – 286

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (RALGPS1B) [UniParc]. Last modified February 5, 2008. Version 1. Checksum: 0C93A55468ED95ED	FASTA	557	62,133
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 220 230 240 EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII 250 260 270 280 290 300 ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE 310 320 330 340 350 360 DLAGPSAGSG SARFSRRPTC PDTSVAGSLP TPPVPRHRKS HSLGNNMMCQ LSVVESKSAT 370 380 390 400 410 420 FPSEKARHLL DDSVLESRSP RRGLALTSSS AVTNGLSLGS SESSEFSEEM SSGLESPTGP 430 440 450 460 470 480 CICSLGNSAA VPTMEGPLRR KTLLKEGRKP ALSSWTRYWV ILSGSTLLYY GAKSLRGTDR 490 500 510 520 530 540 KHYKSTPGKK VSIVGWMVQL PDDPEHPDIF QLNNPDKGNV YKFQTGSRFH AILWHKHLDD 550 ACKSNRPQVP ANLMSFE
	Isoform 2 (RALGPS1A) [UniParc]. Checksum: 49CFB803DABF6BAA	FASTA	529	58,975
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 220 230 240 EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII 250 260 270 280 290 300 ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE 310 320 330 340 350 360 DLAGPSAGSG SARFSRRPTC PDTSVAGSLP TPPVPRHRKS HSLGNNRGRL YATLGPNWRV 370 380 390 400 410 420 PVRNSPRTRS CVYSPTGPCI CSLGNSAAVP TMEGPLRRKT LLKEGRKPAL SSWTRYWVIL 430 440 450 460 470 480 SGSTLLYYGA KSLRGTDRKH VSIVGWMVQL PDDPEHPDIF QLNNPDKGNV YKFQTGSRFH 490 500 510 520 AILWHKHLDD ACKSNRPQEA GAAPGPTGTD SHEVDHLEGG AGKEAGPCA
	Isoform 3 [UniParc]. Checksum: 2B0F6988D850036E	FASTA	384	43,154
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 220 230 240 EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII 250 260 270 280 290 300 ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE 310 320 330 340 350 360 DLAVSHLSSL SHQGQAEEAR LKPTSGQHPA WMWPSSSRVP AAPPASAAPR SPWPRNLRND 370 380 QGQPGAVALT CNPSTLGSRS RRIT
	Isoform 4 [UniParc]. Checksum: A23253E8A5E97706	FASTA	305	34,728
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 220 230 240 EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII 250 260 270 280 290 300 ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE DLAAM
	Isoform 5 [UniParc]. Checksum: 6BE4541C45159E89	FASTA	213	24,325
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 EDNYKRTREY IRSLKMVPSI PYLGRSLNWL IFF
	Isoform 6 [UniParc]. Checksum: 96CA60064D585BB5	FASTA	108	11,772
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 MDIPVFKAIQ PEKGWPARME DSLQKSTVSS RPHDTLQFRK TLPVGPGT
	Isoform 7 [UniParc]. Checksum: 3CCD9778267F43FA	FASTA	537	59,865
	10 20 30 40 50 60 MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT PEEFASQITL 70 80 90 100 110 120 MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ VSFWVVREIL TAQTLKIRAE 130 140 150 160 170 180 ILSHFVKIAK KLLELNNLHS LMSVVSALQS APIFRLTKTW ALLNRKDKTT FEKLDYLMSK 190 200 210 220 230 240 EDNYKRTREY IRSLKMVPSI PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII 250 260 270 280 290 300 ADLQVSCSYD HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE 310 320 330 340 350 360 DLAGPSAGSG SARFSRRPTC PDTSVAGSLP TPPVPRHRKS HSLGNNRGRL YATLGPNWRV 370 380 390 400 410 420 PVRNSPRTRS CVYSPTGPCI CSLGNSAAVP TMEGPLRRKT LLKEGRKPAL SSWTRYWVIL 430 440 450 460 470 480 SGSTLLYYGA KSLRGTDRKH YKSTPGKKVS IVGWMVQLPD DPEHPDIFQL NNPDKGNVYK 490 500 510 520 530 FQTGSRFHAI LWHKHLDDAC KSNRPQEAGA APGPTGTDSH EVDHLEGGAG KEAGPCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral." Rebhun J.F., Chen H., Quilliam L.A. J. Biol. Chem. 275:13406-13410(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH GRB2; NCK1; PLCG1; RALA AND SRC, TISSUE SPECIFICITY. Tissue: Small intestine.
[2]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7).
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3; 4 AND 6). Tissue: Brain and Pancreas.
[7]	"RalGEF2, a pleckstrin homology domain containing guanine nucleotide exchange factor for Ral." de Bruyn K.M., de Rooij J., Wolthuis R.M., Rehmann H., Wesenbeek J., Cool R.H., Wittinghofer A.H., Bos J.L. J. Biol. Chem. 275:29761-29766(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-148.
[8]	"Structural study of the Cdc25 domain from Ral-specific guanine-nucleotide exchange factor RalGPS1a." Peng W., Xu J., Guan X., Sun Y., Zhang X.C., Li X., Rao Z. Protein Cell 2:308-319(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) OF 23-289.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF221098 mRNA. Translation: AAF65253.1.
AB002349 mRNA. Translation: BAA20808.2. Different initiation.
AK299149 mRNA. Translation: BAG61198.1.
AL357623, AL160169, AL356862 Genomic DNA. Translation: CAH72699.1.
AL357623 , AL160169, AL356862, AL450263 Genomic DNA. Translation: CAH72700.1.
AL357623 , AL160169, AL356862, AL450263 Genomic DNA. Translation: CAH72701.1.
AL357623, AL160169, AL356862 Genomic DNA. Translation: CAH72702.1.
AL356862, AL160169, AL357623 Genomic DNA. Translation: CAI39718.1.
AL356862 , AL160169, AL357623, AL450263 Genomic DNA. Translation: CAI39719.1.
AL356862 , AL160169, AL357623, AL450263 Genomic DNA. Translation: CAI39720.1.
AL356862, AL160169, AL357623 Genomic DNA. Translation: CAI39721.1.
AL356862, AL160169 Genomic DNA. Translation: CAI39723.1.
AL160169, AL356862, AL357623 Genomic DNA. Translation: CAI40638.1.
AL160169 , AL356862, AL357623, AL450263 Genomic DNA. Translation: CAI40639.1.
AL160169 , AL356862, AL357623, AL450263 Genomic DNA. Translation: CAI40640.1.
AL160169, AL356862, AL357623 Genomic DNA. Translation: CAI40641.1.
AL160169, AL356862 Genomic DNA. Translation: CAI40643.1.
AL160169 Genomic DNA. Translation: CAI40644.1.
AL450263 , AL160169, AL356862, AL357623 Genomic DNA. Translation: CAI41460.1.
AL450263 , AL160169, AL356862, AL357623 Genomic DNA. Translation: CAI41461.1.
CH471090 Genomic DNA. Translation: EAW87645.1.
CH471090 Genomic DNA. Translation: EAW87646.1.
CH471090 Genomic DNA. Translation: EAW87648.1.
BC019329 mRNA. Translation: AAH19329.1. Different initiation.
BC032372 mRNA. Translation: AAH32372.1.
BC033708 mRNA. Translation: AAH33708.1.

IPI

IPI00041588.
IPI00103033.
IPI00166762.
IPI00329517.
IPI00641854.
IPI00644546.
IPI00942454.

RefSeq

NP_001177657.1. NM_001190728.1.
NP_001177658.1. NM_001190729.1.
NP_001177659.1. NM_001190730.1.
NP_055451.1. NM_014636.2.

UniGene

Hs.432842.
Hs.606576.
Hs.721343.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3QXL	X-ray	2.24	A/B	23-289	[»]

ProteinModelPortal

Q5JS13.

ModBase

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Protein-protein interaction databases

IntAct

Q5JS13. 1 interaction.

PTM databases

PhosphoSite

Q5JS13.

Polymorphism databases

DMDM

189040074.

Proteomic databases

PaxDb

Q5JS13.

PRIDE

Q5JS13.

Protocols and materials databases

StructuralBiologyKnowledgebase

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Genome annotation databases

Ensembl

ENST00000259351; ENSP00000259351; ENSG00000136828.
ENST00000373434; ENSP00000362533; ENSG00000136828.
ENST00000373436; ENSP00000362535; ENSG00000136828.
ENST00000373439; ENSP00000362538; ENSG00000136828.
ENST00000394011; ENSP00000377579; ENSG00000136828.
ENST00000394022; ENSP00000377590; ENSG00000136828.
ENST00000424082; ENSP00000415630; ENSG00000136828.

GeneID

9649.

KEGG

hsa:9649.

UCSC

uc004bqo.2. human.
uc004bqq.4. human.
uc011mab.2. human.
uc022bno.1. human.

Organism-specific databases

CTD

9649.

GeneCards

GC09P129679.

HGNC

HGNC:16851. RALGPS1.

MIM

614444. gene.

neXtProt

NX_Q5JS13.

PharmGKB

PA134907502.

HUGE

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GenAtlas

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Phylogenomic databases

eggNOG

NOG275669.

HOVERGEN

HBG059258.

InParanoid

Q5JS13.

OMA

SCNYDHL.

OrthoDB

EOG41C6W1.

Gene expression databases

ArrayExpress

Q5JS13.

Bgee

Q5JS13.

CleanEx

HS_RALGPS1.

Genevestigator

Q5JS13.

Family and domain databases

Gene3D

1.10.840.10. 1 hit.
2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR008937. Ras_GEF.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view]

PANTHER

PTHR23113. PTHR23113. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF00617. RasGEF. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
[Graphical view]

SUPFAM

SSF48366. Ras_GEF. 1 hit.

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS00720. RASGEF. False negative.
PS50009. RASGEF_CAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

9649.

NextBio

36221.

SOURCE

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Entry information

Entry name

RGPS1_HUMAN

Accession

Primary (citable) accession number: Q5JS13
Secondary accession number(s): B4DR86 , E9PBQ5, O15059, Q5JT60, Q5JT65, Q5JUG5, Q8N4S6, Q8N5H4, Q8WUV7, Q9NZ16

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.