Q5JR59 (MTUS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated tumor suppressor candidate 2

Alternative name(s):
Cardiac zipper protein
Microtubule plus-end tracking protein TIP150
Short name=Tracking protein of 150 kDa

Gene names

Name:	MTUS2
Synonyms:	CAZIP, KIAA0774, TIP150

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1369 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip. Ref.7
Subunit structure	Homodimer. Interacts with KIF2C and MAPRE1; the interaction is direct and probably targets MTUS2 and KIF2C to microtubules. Ref.7
Subcellular location	Cytoplasm › cytoskeleton. Note: Associated with the microtubule network at the growing distal tip (the plus-end) of microtubules. Ref.7
Tissue specificity	Detected in embryonic stem cells differentiating to cardiomyocytes. Ref.6
Sequence similarities	In the C-terminal section; belongs to the MTUS1 family.
Sequence caution	The sequence AAI50246.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA34494.2 differs from that shown. Reason: Erroneous initiation. The sequence CAI39910.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI39912.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI40386.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI40565.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI40566.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI41204.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	microtubule binding Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5JR59-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5JR59-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1021: Missing.
     1022-1039: AICGFDALAVATQHFFRK → MGHCCCKPYNCLQCLDKT

Note: No experimental confirmation available.

Isoform 3 (identifier: Q5JR59-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1111: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1369	1369	Microtubule-associated tumor suppressor candidate 2		PRO_0000280111
Regions
	Region	641 – 980	340	Mediates interaction with MAPRE1
	Region	801 – 1150	350	Localization to the growing distal tip of microtubules
	Region	801 – 890	90	Sufficient for interaction with KIF2C
	Coiled coil	991 – 1335	345	Potential
Natural variations
	Alternative sequence	1 – 1111	1111	Missing in isoform 3.		VSP_023540
	Alternative sequence	1 – 1021	1021	Missing in isoform 2.		VSP_023541
	Alternative sequence	1022 – 1039	18	AICGF…HFFRK → MGHCCCKPYNCLQCLDKT in isoform 2.		VSP_023543
	Natural variant	955	1	P → H. Corresponds to variant rs12874207 [ dbSNP | Ensembl ].		VAR_055941
	Natural variant	1071	1	L → Q. Corresponds to variant rs17073511 [ dbSNP | Ensembl ].		VAR_058831
Experimental info
	Sequence conflict	942	1	Q → P in BAA34494. Ref.1
	Sequence conflict	942	1	Q → P in AAI50246. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 22, 2009. Version 3. Checksum: 4BF7A754AB1ED7CF	FASTA	1,369	150,195
	10 20 30 40 50 60 MSVPVAPKKS CYTQLRDNRN AARNNNESIL SLGDTNANQI MLEVSSSHDE SKTCDLGDEI 70 80 90 100 110 120 GNTNSSEPEN RTHFHKEFHQ LQGFGKGSQA GSASLKDFRL SSTIQRELNE EHTVERGTDS 130 140 150 160 170 180 LQTTRSIQGP SLSSWRNVMS EASLDVLAKR DAEIPRHVPK DKLAKTLDNE ELRRHSLERA 190 200 210 220 230 240 SSSVAAVGSL TPQHPQPLSL DSREARGQIP GGGEGPQKTL PDHAVPAAFP ATDSTSEGKS 250 260 270 280 290 300 VRHPKPSTSE SKQSTPSETQ TVGAHVLQVC SEHTSHSAHP EPALNLTLAS KEIPSKLEAQ 310 320 330 340 350 360 LGQGKGEAKL DLKYVPPRRV EQEGKAAQEG YLGCHKEENL SALEGRDPCG EAHPEATDAL 370 380 390 400 410 420 GHLLNSDLHH LGVGRGNCEE KRGVNPGEQD SLHTTPKQGS ASLGGADNQP TGKISPCAGE 430 440 450 460 470 480 KLGERTSSSF SPGDSHVAFI PNNLTDSKPL DVIEEERRLG SGNKDSVMVL VFNPSVGENK 490 500 510 520 530 540 TEVPEPLDPQ SGRSEARESK EVTTSVAENR NLLENADKIE STSARADSVL NIPAPLHPET 550 560 570 580 590 600 TVNMTYQPTT PSSSFQDVSV FGMDAGSPLV VPPPTDSARL LNTSPKVPDK NTCPSGIPKP 610 620 630 640 650 660 VFTHSKDTPS SQEGMENYQV EKTEERTETK PIIMPKPKHV RPKIITYIRR NPQALGQVDA 670 680 690 700 710 720 SLVPVGLPYA PPTCTMPLPH EEKAAGGDLK PSANLYEKFK PDLQKPRVFS SGLMVSGIKP 730 740 750 760 770 780 PGHPFSQMSE KFLQEVTDHP GKEEFCSPPY AHYEVPPTFY RSAMLLKPQL GLGAMSRLPS 790 800 810 820 830 840 AKSRILIASQ RSSASAIHPP GPITTATSLY SSDPSADLKK ASSSNAAKSN LPKSGLRPPG 850 860 870 880 890 900 YSRLPAAKLA AFGFVRSSSV SSVSSTQSGD SAQPEQGRPA TRSTFGNEEQ PVLKASLPSK 910 920 930 940 950 960 DTPKGAGRVA PPASSSVTAP RRSLLPAPKS TSTPAGTKKD AQKDQDTNKP AVSSPKRVAA 970 980 990 1000 1010 1020 STTKLHSPGY PKQRTAAARN GFPPKPDPQA REAERQLVLR LKERCEQQTR QLGVAQGELK 1030 1040 1050 1060 1070 1080 RAICGFDALA VATQHFFRKN ESALVKEKEL SIELANIRDE VAFHTAKCEK LQKEKEELER 1090 1100 1110 1120 1130 1140 RFEDEVKRLG WQQQAELQEL EERLQLQFEA EMARLQEEHG DQLLSIRCQH QEQVEDLTAS 1150 1160 1170 1180 1190 1200 HDAALLEMEN NHTVAITILQ DDHDHKVQEL MSTHELEKKE LEENFEKLRL SLQDQVDTLT 1210 1220 1230 1240 1250 1260 FQSQSLRDRA RRFEEALRKN TEEQLEIALA PYQHLEEDMK SLKQVLEMKN QQIHEQEKKI 1270 1280 1290 1300 1310 1320 LELEKLAEKN IILEEKIQVL QQQNEDLKAR IDQNTVVTRQ LSEENANLQE YVEKETQEKK 1330 1340 1350 1360 RLSRTNEELL WKLQTGDPTS PIKLSPTSPV YRGSSSGPSS PARVSTTPR
	Isoform 2 [UniParc]. Checksum: D352305A4FA0566B	FASTA	348	40,746
	10 20 30 40 50 60 MGHCCCKPYN CLQCLDKTNE SALVKEKELS IELANIRDEV AFHTAKCEKL QKEKEELERR 70 80 90 100 110 120 FEDEVKRLGW QQQAELQELE ERLQLQFEAE MARLQEEHGD QLLSIRCQHQ EQVEDLTASH 130 140 150 160 170 180 DAALLEMENN HTVAITILQD DHDHKVQELM STHELEKKEL EENFEKLRLS LQDQVDTLTF 190 200 210 220 230 240 QSQSLRDRAR RFEEALRKNT EEQLEIALAP YQHLEEDMKS LKQVLEMKNQ QIHEQEKKIL 250 260 270 280 290 300 ELEKLAEKNI ILEEKIQVLQ QQNEDLKARI DQNTVVTRQL SEENANLQEY VEKETQEKKR 310 320 330 340 LSRTNEELLW KLQTGDPTSP IKLSPTSPVY RGSSSGPSSP ARVSTTPR
	Isoform 3 [UniParc]. Checksum: 83363B0586426D1A	FASTA	258	30,064
	10 20 30 40 50 60 MARLQEEHGD QLLSIRCQHQ EQVEDLTASH DAALLEMENN HTVAITILQD DHDHKVQELM 70 80 90 100 110 120 STHELEKKEL EENFEKLRLS LQDQVDTLTF QSQSLRDRAR RFEEALRKNT EEQLEIALAP 130 140 150 160 170 180 YQHLEEDMKS LKQVLEMKNQ QIHEQEKKIL ELEKLAEKNI ILEEKIQVLQ QQNEDLKARI 190 200 210 220 230 240 DQNTVVTRQL SEENANLQEY VEKETQEKKR LSRTNEELLW KLQTGDPTSP IKLSPTSPVY 250 RGSSSGPSSP ARVSTTPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cerebellum.
[4]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Pancreas.
[6]	"CAZIP, a novel protein expressed in the developing heart and nervous system." Du Puy L., Beqqali A., Monshouwer-Kloots J., Haagsman H.P., Roelen B.A., Passier R. Dev. Dyn. 238:2903-2911(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"TIP150 interacts with and targets MCAK at the microtubule plus ends." Jiang K., Wang J., Liu J., Ward T., Wordeman L., Davidson A., Wang F., Yao X. EMBO Rep. 10:857-865(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KIF2C AND MAPRE1, HOMODIMERIZATION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018317 mRNA. Translation: BAA34494.2. Different initiation. AK293970 mRNA. Translation: BAG57342.1. AL596114 , AL160255, AL596092, AL607144 Genomic DNA. Translation: CAI39910.1. Sequence problems. AL596114, AL607144 Genomic DNA. Translation: CAI39912.1. Sequence problems. AL596092 , AL160255, AL596114, AL607144 Genomic DNA. Translation: CAI40386.1. Sequence problems. AL607144 , AL160255, AL596092, AL596114 Genomic DNA. Translation: CAI40565.1. Sequence problems. AL607144, AL596114 Genomic DNA. Translation: CAI40566.1. Sequence problems. AL160255 , AL596092, AL596114, AL607144 Genomic DNA. Translation: CAI41204.1. Sequence problems. BC032481 mRNA. Translation: AAH32481.1. BC119654 mRNA. Translation: AAI19655.1. BC119655 mRNA. Translation: AAI19656.1. BC129985 mRNA. Translation: AAI29986.1. BC150245 mRNA. Translation: AAI50246.1. Different initiation.
IPI	IPI00645435. IPI00829933. IPI01012982.
RefSeq	NP_001028774.2. NM_001033602.2. NP_056048.1. NM_015233.5.
UniGene	Hs.22287.
3D structure databases
ProteinModelPortal	Q5JR59.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5JR59. 6 interactions.
MINT	MINT-1457050.
STRING	9606.ENSP00000255289.
PTM databases
PhosphoSite	Q5JR59.
Polymorphism databases
DMDM	259016371.
Proteomic databases
PaxDb	Q5JR59.
PRIDE	Q5JR59.
Protocols and materials databases
DNASU	23281.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380808; ENSP00000370186; ENSG00000132938. ENST00000542829; ENSP00000445403; ENSG00000132938.
GeneID	23281.
KEGG	hsa:23281.
UCSC	uc001usl.4. human. uc001usm.4. human. uc010aau.3. human.
Organism-specific databases
CTD	23281.
GeneCards	GC13P029599.
H-InvDB	HIX0020942.
HGNC	HGNC:20595. MTUS2.
HPA	HPA031474. HPA031475.
neXtProt	NX_Q5JR59.
PharmGKB	PA165505194.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG119660.
InParanoid	Q5JR59.
OrthoDB	EOG4S1T7T.
Gene expression databases
ArrayExpress	Q5JR59.
Bgee	Q5JR59.
CleanEx	HS_KIAA0774.
Genevestigator	Q5JR59.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	MTUS2. human.
GenomeRNAi	23281.
NextBio	45074.

Entry information

Entry name

MTUS2_HUMAN

Accession

Primary (citable) accession number: Q5JR59
Secondary accession number(s): A7E292 , B4DF81, O94872, Q08E97, Q5JQR3, Q8N5E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	September 22, 2009
Last modified:	April 3, 2013
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.