Q5JQC9 (AKAP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 75.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: A-kinase anchor protein 4 Short name=AKAP-4 Alternative name(s): A-kinase anchor protein 82 kDa Short name=AKAP 82 Short name=hAKAP82 Major sperm fibrous sheath protein Short name=HI Protein kinase A-anchoring protein 4 Short name=PRKA4 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 854 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Ref.2 |
| Subunit structure | Interacts with PRKAR1A and PRKAR2A By similarity. UniProtKB Q60662 |
| Subcellular location | Cell projection › cilium › flagellum. Note: Localizes to the principle piece of the sperm flagellum. Ref.2 |
| Tissue specificity | Testis specific; only expressed in round spermatids. Ref.1 |
| Developmental stage | Post-meiotic phase of spermatogenesis. Ref.1 |
| Domain | RI-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer By similarity. UniProtKB Q60662 |
| Sequence similarities | Belongs to the AKAP110 family. |
| Sequence caution | The sequence CAA75494.1 differs from that shown. Reason: Frameshift at several positions. |
Ontologies
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.1 (identifier: Q5JQC9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 Ref.2 (identifier: Q5JQC9-2) The sequence of this isoform differs from the canonical sequence as follows: 1-9: Missing. | ||||||
| Isoform 3 Ref.3 (identifier: Q5JQC9-3) The sequence of this isoform differs from the canonical sequence as follows: 1-9: Missing. 191-564: Missing. | ||||||
| Note: Gene prediction based on EST data. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 188 | 188 | Potential | PRO_0000248230 | |||||
| Chain | 189 – 854 | 666 | A-kinase anchor protein 4 UniProtKB Q60662 | PRO_0000248231 | |||||
Regions | |||||||||
| Region | 219 – 232 | 14 | PKA-RI and PKA-RII subunit binding domain By similarity UniProtKB Q60662 | ||||||
| Region | 336 – 345 | 10 | PKA-RI-alpha subunit binding domain By similarity UniProtKB Q60662 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 9 | 9 | Missing in isoform 2 and isoform 3. Ref.2 Ref.3 | VSP_052142 | |||||
| Alternative sequence | 191 – 564 | 374 | Missing in isoform 3. Ref.3 | VSP_052143 | |||||
| Natural variant | 233 | 1 | H → R. Corresponds to variant rs17174078 [ dbSNP | Ensembl ]. | VAR_048206 | |||||
| Natural variant | 673 | 1 | A → G. Corresponds to variant rs12012704 [ dbSNP | Ensembl ]. | VAR_027266 | |||||
Experimental info | |||||||||
| Sequence conflict | 123 | 1 | L → W in AAC79433. Ref.2 | ||||||
| Sequence conflict | 128 | 1 | A → E in AAC79433. Ref.2 | ||||||
| Sequence conflict | 146 | 1 | E → D in AAC79433. Ref.2 | ||||||
| Sequence conflict | 190 | 1 | S → N in CAI41558. Ref.3 | ||||||
| Sequence conflict | 369 | 1 | R → W in CAA75494. Ref.1 | ||||||
| Sequence conflict | 428 – 429 | 2 | KR → IL in AAC79433. Ref.2 | ||||||
| Sequence conflict | 517 | 1 | K → M in AAC79433. Ref.2 | ||||||
| Sequence conflict | 560 | 1 | T → I in CAA75494. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of human testis mRNA specifically expressed in haploid germ cells, having structural homology with the A-kinase anchoring proteins." Mohapatra B., Verma S., Shankar S., Suri A. Biochem. Biophys. Res. Commun. 244:540-545(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Testis. |
| [2] | "An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail." Turner R.M.O., Johnson L.R., Haig-Ladewig L., Gerton G.L., Moss S.B. J. Biol. Chem. 273:32135-32141(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION. |
| [3] | "The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. Bentley D.R.Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3). |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y15195 mRNA. Translation: CAA75494.1. Sequence problems. AF072756 mRNA. Translation: AAC79433.1. AL663119 Genomic DNA. Translation: CAI41557.1. AL663119 Genomic DNA. Translation: CAI41558.1. AL663119 Genomic DNA. Translation: CAI41559.1. AL663119 Genomic DNA. Translation: CAI41561.1. BC126250 mRNA. Translation: AAI26251.1. BC126252 mRNA. Translation: AAI26253.1. |
| IPI | IPI00157860. IPI00333264. IPI00552135. |
| PIR | JC5986. |
| RefSeq | NP_003877.2. NM_003886.2. NP_647450.1. NM_139289.1. |
| UniGene | Hs.97633. |
3D structure databases | |
| ProteinModelPortal | Q5JQC9. |
| SMR | Q5JQC9. Positions 771-853. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 9606.ENSP00000351327. |
PTM databases | |
| PhosphoSite | Q5JQC9. |
Polymorphism databases | |
| DMDM | 74741729. |
Proteomic databases | |
| PaxDb | Q5JQC9. |
| PRIDE | Q5JQC9. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000358526; ENSP00000351327; ENSG00000147081. ENST00000376056; ENSP00000365224; ENSG00000147081. ENST00000376064; ENSP00000365232; ENSG00000147081. ENST00000437370; ENSP00000412279; ENSG00000147081. ENST00000448865; ENSP00000402403; ENSG00000147081. ENST00000594184; ENSP00000472101; ENSG00000269285. ENST00000594628; ENSP00000471711; ENSG00000269285. ENST00000594732; ENSP00000469992; ENSG00000269285. ENST00000596010; ENSP00000470803; ENSG00000269285. ENST00000601454; ENSP00000473209; ENSG00000269285. |
| GeneID | 8852. |
| KEGG | hsa:8852. |
| UCSC | uc004dou.1. human. |
Organism-specific databases | |
| CTD | 8852. |
| GeneCards | GC0XM049955. |
| HGNC | HGNC:374. AKAP4. |
| HPA | HPA005949. HPA020046. |
| MIM | 300185. gene. |
| neXtProt | NX_Q5JQC9. |
| PharmGKB | PA24668. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | NOG40269. |
| HOGENOM | HOG000220883. |
| HOVERGEN | HBG050478. |
| InParanoid | Q5JQC9. |
| KO | K16521. |
| OMA | SKGLMVY. |
| OrthoDB | EOG4JQ3WX. |
| PhylomeDB | Q5JQC9. |
Gene expression databases | |
| ArrayExpress | Q5JQC9. |
| Bgee | Q5JQC9. |
| CleanEx | HS_AKAP4. |
| Genevestigator | Q5JQC9. |
| GermOnline | ENSG00000147081. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR020799. AKAP_110. IPR018292. AKAP_110_C. IPR018459. RII_binding_1. IPR008382. SPHK1-interactor_AKAP_110. [Graphical view] |
| PANTHER | PTHR10226. PTHR10226. 1 hit. |
| Pfam | PF05716. AKAP_110. 1 hit. PF10522. RII_binding_1. 1 hit. [Graphical view] |
| SMART | SM00807. AKAP_110. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| ChiTaRS | AKAP4. human. |
| GenomeRNAi | 8852. |
| NextBio | 33231. |
| SOURCE | Search... |
Entry information
| Entry name | AKAP4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q5JQC9 Secondary accession number(s): A0AV85 Q5JQD3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome X Human chromosome X: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
