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Q5JN63 (CESA4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cellulose synthase A catalytic subunit 4 [UDP-forming]
EC=2.4.1.12
Alternative name(s):
OsCesA4
Gene names
Name:CESA4
Ordered Locus Names:Os01g0750300, LOC_Os01g54620
ORF Names:P0046E05.4
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length989 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity. Involved in the secondary cell wall formation. Ref.5 Ref.6

Catalytic activity

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Cofactor

Binds 2 zinc ions per subunit By similarity.

Pathway

Glycan metabolism; plant cellulose biosynthesis.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Disruption phenotype

Plants develop a brittle culm (bc) phenotype with a reduction of up to 80 percent of cellulose content in culm. Ref.5 Ref.6

Sequence similarities

Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.

Contains 1 RING-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 989989Cellulose synthase A catalytic subunit 4 [UDP-forming]
PRO_0000319363

Regions

Topological domain1 – 184184Cytoplasmic Potential
Transmembrane185 – 20521Helical; Potential
Topological domain206 – 2138Extracellular Potential
Transmembrane214 – 23421Helical; Potential
Topological domain235 – 772538Cytoplasmic Potential
Transmembrane773 – 79321Helical; Potential
Topological domain794 – 7985Extracellular Potential
Transmembrane799 – 81921Helical; Potential
Topological domain820 – 83516Cytoplasmic Potential
Transmembrane836 – 85621Helical; Potential
Topological domain857 – 88428Extracellular Potential
Transmembrane885 – 90521Helical; Potential
Topological domain906 – 91611Cytoplasmic Potential
Transmembrane917 – 93721Helical; Potential
Topological domain938 – 9469Extracellular Potential
Transmembrane947 – 96721Helical; Potential
Topological domain968 – 98922Cytoplasmic Potential
Zinc finger9 – 4739RING-type; degenerate
Coiled coil362 – 38928 Potential

Sites

Active site3081 Potential
Active site6881 Potential
Metal binding91Zinc 1 By similarity
Metal binding121Zinc 1 By similarity
Metal binding201Zinc 2 By similarity
Metal binding231Zinc 2 By similarity
Metal binding281Zinc 1 By similarity
Metal binding311Zinc 1 By similarity
Metal binding431Zinc 2 By similarity
Metal binding461Zinc 2 By similarity
Binding site4741Substrate Potential
Binding site4761Substrate Potential

Amino acid modifications

Glycosylation8621N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q5JN63 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 0A504424A94B2E9C

FASTA989111,027
        10         20         30         40         50         60 
MMESGVPPCA ACGDDAHAAC RACSYALCKA CLDEDAAEGR TTCARCGGEY GAPDPAHGQG 

        70         80         90        100        110        120 
AVVEEEVEES HEPAAGGVRE RVTMASQLSD HQDEGVHART MSTHARTISS VSGVGSELND 

       130        140        150        160        170        180 
ESGKPIWKNR VESWKEKKKE KKASAKKAAA KAQAPPVEEQ IMDEKDLTDA YEPLSRIIPI 

       190        200        210        220        230        240 
SKNKLTPYRA VIIMRLVVLG LFFHYRITNP VYSAFGLWMT SVICEIWFGF SWILDQFPKW 

       250        260        270        280        290        300 
CPINRETYVD RLIARYGDGE DSGLAPVDFF VSTVDPLKEP PLITANTVLS ILAVDYPVEK 

       310        320        330        340        350        360 
ISCYVSDDGS AMLTFESLAE TAEFARRWVP FCKKYSIEPR APEFYFSQKI DYLKDKIHPS 

       370        380        390        400        410        420 
FVKERRAMKR DYEEYKVRIN ALVAKAQKTP EEGWIMQDGT PWPGNNPRDH PGMIQVFLGE 

       430        440        450        460        470        480 
TGARDFDGNE LPRLVYVSRE KRPGYQHHKK AGAMNALVRV SAVLTNAPYI LNLDCDHYVN 

       490        500        510        520        530        540 
NSKAVREAMC FMMDPSVGRD VCYVQFPQRF DGIDRSDRYA NRNVVFFDVN MKGLDGLQGP 

       550        560        570        580        590        600 
VYVGTGCCFY RQALYGYGPP SLPALPKSSV CSWCCCCCPK KKAEKSEKEM HRDSRREDLE 

       610        620        630        640        650        660 
SAIFNLREID NYDEYERSML ISQMSFEKSF GLSSVFIEST LMENGGVPES ANPSTLIKEA 

       670        680        690        700        710        720 
IHVISCGYEE KTEWGKEIGW IYGSVTEDIL TGFKMHCRGW RSIYCMPIRP AFKGSAPINL 

       730        740        750        760        770        780 
SDRLHQVLRW ALGSVEIFLS RHCPLWYGYG GGRLKWLQRL SYINTIVYPF TSLPLIAYCC 

       790        800        810        820        830        840 
LPAICLLTGK FIIPTLSNAA TIWFLGLFIS IIVTSVLELR WSGIGIEDWW RNEQFWVIGG 

       850        860        870        880        890        900 
VSAHLFAVFQ GILKMIAGLD TNFTVTAKAT DDTEFGELYV FKWTTVLIPP TSILVLNLVG 

       910        920        930        940        950        960 
VVAGFSDALN SGYESWGPLF GKVFFAMWVI MHLYPFLKGL MGRQNRTPTI VVLWSVLLAS 

       970        980 
VFSLLWVKID PFIGSSETTT TNSCANFDC

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed: 12447438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[5]"Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall."
Tanaka K., Murata K., Yamazaki M., Onosato K., Miyao A., Hirochika H.
Plant Physiol. 133:73-83(2003) [PubMed: 12970476] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[6]"Fine mapping and isolation of Bc7(t), allelic to OsCesA4."
Yan C., Yan S., Zeng X., Zhang Z., Gu M.
J. Genet. Genomics 34:1019-1027(2007) [PubMed: 18037139] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP003237 Genomic DNA. Translation: BAD87094.1.
AP008207 Genomic DNA. Translation: BAF06166.1.
AK100475 mRNA. No translation available.
RefSeqNP_001044252.1. NM_001050787.1.
UniGeneOs.18724.

3D structure databases

ProteinModelPortalQ5JN63.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5JN63.

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4324625.
KEGGosa:4324625.

Organism-specific databases

GrameneQ5JN63.

Phylogenomic databases

HOGENOMHBG744549.
OMAKVFFAFW.
PhylomeDBQ5JN63.
ProtClustDBPLN02195.

Family and domain databases

InterProIPR005150. Cellulose_synth.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
Gene3DG3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KOK10999.
PfamPF03552. Cellulose_synt. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCESA4_ORYSJ
AccessionPrimary (citable) accession number: Q5JN63
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 15, 2005
Last modified: December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents