Reviewed, UniProtKB/Swiss-Prot Q5JN63 (CESA4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cellulose synthase A catalytic subunit 4 [UDP-forming]
    EC=2.4.1.12
Alternative name(s):
    OsCesA4

Gene names

Name:	CESA4
Ordered Locus Names:	Os01g0750300, LOC_Os01g54620
ORF Names:	P0046E05.4

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	989 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity. Involved in the secondary cell wall formation.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Disruption phenotype	Plants develop a brittle culm (bc) phenotype with a reduction of up to 80 percent of cellulose content in culm. Ref.5 Ref.6
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 989	989	Cellulose synthase A catalytic subunit 4 [UDP-forming]		PRO_0000319363
Regions
	Topological domain	1 – 184	184	Cytoplasmic Potential
	Transmembrane	185 – 205	21	Potential
	Topological domain	206 – 213	8	Extracellular Potential
	Transmembrane	214 – 234	21	Potential
	Topological domain	235 – 772	538	Cytoplasmic Potential
	Transmembrane	773 – 793	21	Potential
	Topological domain	794 – 798	5	Extracellular Potential
	Transmembrane	799 – 819	21	Potential
	Topological domain	820 – 835	16	Cytoplasmic Potential
	Transmembrane	836 – 856	21	Potential
	Topological domain	857 – 884	28	Extracellular Potential
	Transmembrane	885 – 905	21	Potential
	Topological domain	906 – 916	11	Cytoplasmic Potential
	Transmembrane	917 – 937	21	Potential
	Topological domain	938 – 946	9	Extracellular Potential
	Transmembrane	947 – 967	21	Potential
	Topological domain	968 – 989	22	Cytoplasmic Potential
	Zinc finger	9 – 47	39	RING-type; degenerate
	Coiled coil	362 – 389	28	Potential
Sites
	Active site	308	1	Potential
	Active site	688	1	Potential
	Metal binding	9	1	Zinc 1 By similarity
	Metal binding	12	1	Zinc 1 By similarity
	Metal binding	20	1	Zinc 2 By similarity
	Metal binding	23	1	Zinc 2 By similarity
	Metal binding	28	1	Zinc 1 By similarity
	Metal binding	31	1	Zinc 1 By similarity
	Metal binding	43	1	Zinc 2 By similarity
	Metal binding	46	1	Zinc 2 By similarity
	Binding site	474	1	Substrate Potential
	Binding site	476	1	Substrate Potential
Amino acid modifications
	Glycosylation	862	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5JN63-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 0A504424A94B2E9C	FASTA	989	111,027
	10 20 30 40 50 60 MMESGVPPCA ACGDDAHAAC RACSYALCKA CLDEDAAEGR TTCARCGGEY GAPDPAHGQG 70 80 90 100 110 120 AVVEEEVEES HEPAAGGVRE RVTMASQLSD HQDEGVHART MSTHARTISS VSGVGSELND 130 140 150 160 170 180 ESGKPIWKNR VESWKEKKKE KKASAKKAAA KAQAPPVEEQ IMDEKDLTDA YEPLSRIIPI 190 200 210 220 230 240 SKNKLTPYRA VIIMRLVVLG LFFHYRITNP VYSAFGLWMT SVICEIWFGF SWILDQFPKW 250 260 270 280 290 300 CPINRETYVD RLIARYGDGE DSGLAPVDFF VSTVDPLKEP PLITANTVLS ILAVDYPVEK 310 320 330 340 350 360 ISCYVSDDGS AMLTFESLAE TAEFARRWVP FCKKYSIEPR APEFYFSQKI DYLKDKIHPS 370 380 390 400 410 420 FVKERRAMKR DYEEYKVRIN ALVAKAQKTP EEGWIMQDGT PWPGNNPRDH PGMIQVFLGE 430 440 450 460 470 480 TGARDFDGNE LPRLVYVSRE KRPGYQHHKK AGAMNALVRV SAVLTNAPYI LNLDCDHYVN 490 500 510 520 530 540 NSKAVREAMC FMMDPSVGRD VCYVQFPQRF DGIDRSDRYA NRNVVFFDVN MKGLDGLQGP 550 560 570 580 590 600 VYVGTGCCFY RQALYGYGPP SLPALPKSSV CSWCCCCCPK KKAEKSEKEM HRDSRREDLE 610 620 630 640 650 660 SAIFNLREID NYDEYERSML ISQMSFEKSF GLSSVFIEST LMENGGVPES ANPSTLIKEA 670 680 690 700 710 720 IHVISCGYEE KTEWGKEIGW IYGSVTEDIL TGFKMHCRGW RSIYCMPIRP AFKGSAPINL 730 740 750 760 770 780 SDRLHQVLRW ALGSVEIFLS RHCPLWYGYG GGRLKWLQRL SYINTIVYPF TSLPLIAYCC 790 800 810 820 830 840 LPAICLLTGK FIIPTLSNAA TIWFLGLFIS IIVTSVLELR WSGIGIEDWW RNEQFWVIGG 850 860 870 880 890 900 VSAHLFAVFQ GILKMIAGLD TNFTVTAKAT DDTEFGELYV FKWTTVLIPP TSILVLNLVG 910 920 930 940 950 960 VVAGFSDALN SGYESWGPLF GKVFFAMWVI MHLYPFLKGL MGRQNRTPTI VVLWSVLLAS 970 980 VFSLLWVKID PFIGSSETTT TNSCANFDC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall." Tanaka K., Murata K., Yamazaki M., Onosato K., Miyao A., Hirochika H. Plant Physiol. 133:73-83(2003) [PubMed: 12970476] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[6]	"Fine mapping and isolation of Bc7(t), allelic to OsCesA4." Yan C., Yan S., Zeng X., Zhang Z., Gu M. J. Genet. Genomics 34:1019-1027(2007) [PubMed: 18037139] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003237 Genomic DNA. Translation: BAD87094.1. AP008207 Genomic DNA. Translation: BAF06166.1. AK100475 mRNA. No translation available.
RefSeq	NP_001044252.1.
UniGene	Os.18724
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	4324625.
KEGG	osa:4324625.
Organism-specific databases
Gramene	Q5JN63.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA4_ORYSJ

Accession

Primary (citable) accession number: Q5JN63

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)