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Protein

CBL-interacting protein kinase 8

Gene

CIPK8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting protein kinase 8 (EC:2.7.11.1)
Alternative name(s):
OsCIPK08
Gene namesi
Name:CIPK8
Ordered Locus Names:Os01g0536000, LOC_Os01g35184
ORF Names:OSJNBa0086A10.18
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446CBL-interacting protein kinase 8PRO_0000338366Add
BLAST

Proteomic databases

PaxDbiQ5JLD8.

Expressioni

Inductioni

By salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ5JLD8. baseline and differential.
GenevisibleiQ5JLD8. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g35184.1.

Structurei

3D structure databases

ProteinModelPortaliQ5JLD8.
SMRiQ5JLD8. Positions 306-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 266254Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini301 – 32929NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 18128Activation loopBy similarityAdd
BLAST
Regioni336 – 36530PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ5JLD8.
OMAiEGLNANK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JLD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGGGALRRV GKYEVGRTIG EGTFAKVKFA QNTESGESVA MKVVDRSSIL
60 70 80 90 100
KHKMADQIKR EISIMKLVRH PNVVRLHEVL ASRKKIFIIL EFITGGELFD
110 120 130 140 150
KIIRHGRLNE ADARRYFQQL IDGVDFCHSK GVYHRDLKPE NLLLDSQGNL
160 170 180 190 200
KISDFGLSAW PAQGGALLRT TCGTPNYVAP EVLSHKGYDG ALADTWSCGV
210 220 230 240 250
ILYVLLAGYL PFDEVDLTTL YGKIESAEYS FPAWFPNGAK SLIHRILDPN
260 270 280 290 300
PDKRIRIEEI RNDEWFKKNY EPTREIESEE VNLDDVNAAF DDPEEDADHT
310 320 330 340 350
LDDEAGPLTL NAFDLIILSQ GLNLAALFDR RQDYDKLQNR FLSRKPAKVI
360 370 380 390 400
MSSMEVVAQS MGYKTHIRNY KMRVEGLNAN KTSHLAVMLE IFEVAPSIFM
410 420 430 440
IELQRAAGDT SDYNKFINNY CSKLDDIIWN FPIEKSKSRI SRLSKR
Length:446
Mass (Da):50,598
Last modified:February 15, 2005 - v1
Checksum:i7E821C9C762ED2B6
GO

Sequence cautioni

The sequence AK120431 differs from that shown. Reason: Frameshift at position 219. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003449 Genomic DNA. Translation: BAD87720.1.
AP008207 Genomic DNA. Translation: BAF05173.1.
AP014957 Genomic DNA. Translation: BAS72544.1.
AK120431 mRNA. No translation available.
RefSeqiXP_015621427.1. XM_015765941.1.
UniGeneiOs.13540.

Genome annotation databases

EnsemblPlantsiOS01T0536000-01; OS01T0536000-01; OS01G0536000.
GeneIDi4326419.
GrameneiOS01T0536000-01; OS01T0536000-01; OS01G0536000.
KEGGiosa:4326419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003449 Genomic DNA. Translation: BAD87720.1.
AP008207 Genomic DNA. Translation: BAF05173.1.
AP014957 Genomic DNA. Translation: BAS72544.1.
AK120431 mRNA. No translation available.
RefSeqiXP_015621427.1. XM_015765941.1.
UniGeneiOs.13540.

3D structure databases

ProteinModelPortaliQ5JLD8.
SMRiQ5JLD8. Positions 306-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g35184.1.

Proteomic databases

PaxDbiQ5JLD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0536000-01; OS01T0536000-01; OS01G0536000.
GeneIDi4326419.
GrameneiOS01T0536000-01; OS01T0536000-01; OS01G0536000.
KEGGiosa:4326419.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ5JLD8.
OMAiEGLNANK.

Gene expression databases

ExpressionAtlasiQ5JLD8. baseline and differential.
GenevisibleiQ5JLD8. OS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks."
    Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.
    Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement."
    Xiang Y., Huang Y., Xiong L.
    Plant Physiol. 144:1416-1428(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCIPK8_ORYSJ
AccessioniPrimary (citable) accession number: Q5JLD8
Secondary accession number(s): A0A0P0V3N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 15, 2005
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.