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Protein

Probable protein phosphatase 2C 5

Gene

Os01g0552300

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Manganese 1By similarity
Metal bindingi92 – 921Manganese 2By similarity
Metal bindingi93 – 931Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi295 – 2951Manganese 2By similarity
Metal bindingi338 – 3381Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 5 (EC:3.1.3.16)
Short name:
OsPP2C05
Gene namesi
Ordered Locus Names:Os01g0552300, LOC_Os01g37130
ORF Names:OSJNBa0066C06.19
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 1

Organism-specific databases

GrameneiQ5JKN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei366 – 38823HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Probable protein phosphatase 2C 5PRO_0000363251Add
BLAST

Proteomic databases

PRIDEiQ5JKN1.

Expressioni

Gene expression databases

ExpressionAtlasiQ5JKN1. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g37130.1.

Structurei

3D structure databases

ProteinModelPortaliQ5JKN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 347291PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000029436.
InParanoidiQ5JKN1.
KOiK04457.
OMAiLYKECAA.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5JKN1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLSPRVPR LPLASASAAG AGLRCCVSGG RAGSAAWCHA SAAGSVASSS
60 70 80 90 100
SELEAIRWGT AKLQGARDEM EDEVVLRPGS LLDGFSFAAV FDGHAGFSAV
110 120 130 140 150
EFLRDELYKE CAAALDGGAV LSTKNLDAIT DSIQRAFATV DANLSTWLEQ
160 170 180 190 200
MDKEDESGAT ATAMFLRNDV LVVSHIGDSC LQVVSRGGRP QAVTNFHRPY
210 220 230 240 250
GNKKASLEEV KRIRAAGGWI VDGRICGEIS VSRAFGDIRF KTRKNEMLVK
260 270 280 290 300
GVKEGRWTEK FISRINFKGD LIVSSPDVSL VELGPDVEFV LLATDGLWDY
310 320 330 340 350
IKSSEAVALV RDQLRQHGDV QVACEALGQI ALDRRSQDNI SIVIADLGRT
360 370 380
NWKELPAQRP NLFLELTQAV ATVGAVSLGI YISSLLALQ
Length:389
Mass (Da):41,738
Last modified:February 15, 2005 - v1
Checksum:iA22EF6515BCF0C01
GO
Isoform 2 (identifier: Q5JKN1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-182: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:388
Mass (Da):41,610
Checksum:i407CB5417AC44BEF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441G → S in AK106278 (PubMed:12869764).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei182 – 1821Missing in isoform 2. CuratedVSP_036258

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003933 Genomic DNA. Translation: BAD87977.1.
AP008207 Genomic DNA. Translation: BAF05225.1.
AK106278 mRNA. No translation available.
RefSeqiNP_001043311.1. NM_001049846.1. [Q5JKN1-1]
UniGeneiOs.15194.

Genome annotation databases

EnsemblPlantsiOS01T0552300-01; OS01T0552300-01; OS01G0552300. [Q5JKN1-1]
GeneIDi4325851.
KEGGiosa:4325851.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003933 Genomic DNA. Translation: BAD87977.1.
AP008207 Genomic DNA. Translation: BAF05225.1.
AK106278 mRNA. No translation available.
RefSeqiNP_001043311.1. NM_001049846.1. [Q5JKN1-1]
UniGeneiOs.15194.

3D structure databases

ProteinModelPortaliQ5JKN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g37130.1.

Proteomic databases

PRIDEiQ5JKN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0552300-01; OS01T0552300-01; OS01G0552300. [Q5JKN1-1]
GeneIDi4325851.
KEGGiosa:4325851.

Organism-specific databases

GrameneiQ5JKN1.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000029436.
InParanoidiQ5JKN1.
KOiK04457.
OMAiLYKECAA.

Gene expression databases

ExpressionAtlasiQ5JKN1. baseline.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Nipponbare.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C05_ORYSJ
AccessioniPrimary (citable) accession number: Q5JKN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.