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Q5JKN1

- P2C05_ORYSJ

UniProt

Q5JKN1 - P2C05_ORYSJ

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Protein

Probable protein phosphatase 2C 5

Gene
Os01g0552300, LOC_Os01g37130, OSJNBa0066C06.19
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Manganese 1 By similarity
Metal bindingi92 – 921Manganese 2 By similarity
Metal bindingi93 – 931Manganese 1; via carbonyl oxygen By similarity
Metal bindingi295 – 2951Manganese 2 By similarity
Metal bindingi338 – 3381Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. photosynthetic electron transport chain Source: EnsemblPlants/Gramene
  2. photosystem stoichiometry adjustment Source: EnsemblPlants/Gramene
  3. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 5 (EC:3.1.3.16)
Short name:
OsPP2C05
Gene namesi
Ordered Locus Names:Os01g0552300, LOC_Os01g37130
ORF Names:OSJNBa0066C06.19
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ5JKN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei366 – 38823Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleolus Source: EnsemblPlants/Gramene
  4. thylakoid Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Probable protein phosphatase 2C 5PRO_0000363251Add
BLAST

Proteomic databases

PRIDEiQ5JKN1.

Structurei

3D structure databases

ProteinModelPortaliQ5JKN1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 340285PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000029436.
KOiK04457.
OMAiIVNGRIC.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5JKN1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALLSPRVPR LPLASASAAG AGLRCCVSGG RAGSAAWCHA SAAGSVASSS    50
SELEAIRWGT AKLQGARDEM EDEVVLRPGS LLDGFSFAAV FDGHAGFSAV 100
EFLRDELYKE CAAALDGGAV LSTKNLDAIT DSIQRAFATV DANLSTWLEQ 150
MDKEDESGAT ATAMFLRNDV LVVSHIGDSC LQVVSRGGRP QAVTNFHRPY 200
GNKKASLEEV KRIRAAGGWI VDGRICGEIS VSRAFGDIRF KTRKNEMLVK 250
GVKEGRWTEK FISRINFKGD LIVSSPDVSL VELGPDVEFV LLATDGLWDY 300
IKSSEAVALV RDQLRQHGDV QVACEALGQI ALDRRSQDNI SIVIADLGRT 350
NWKELPAQRP NLFLELTQAV ATVGAVSLGI YISSLLALQ 389
Length:389
Mass (Da):41,738
Last modified:February 15, 2005 - v1
Checksum:iA22EF6515BCF0C01
GO
Isoform 2 (identifier: Q5JKN1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-182: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:388
Mass (Da):41,610
Checksum:i407CB5417AC44BEF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei182 – 1821Missing in isoform 2. VSP_036258

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441G → S in AK106278. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003933 Genomic DNA. Translation: BAD87977.1.
AP008207 Genomic DNA. Translation: BAF05225.1.
AK106278 mRNA. No translation available.
RefSeqiNP_001043311.1. NM_001049846.1. [Q5JKN1-1]
UniGeneiOs.15194.

Genome annotation databases

EnsemblPlantsiOS01T0552300-01; OS01T0552300-01; OS01G0552300. [Q5JKN1-1]
GeneIDi4325851.
KEGGiosa:4325851.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003933 Genomic DNA. Translation: BAD87977.1 .
AP008207 Genomic DNA. Translation: BAF05225.1 .
AK106278 mRNA. No translation available.
RefSeqi NP_001043311.1. NM_001049846.1. [Q5JKN1-1 ]
UniGenei Os.15194.

3D structure databases

ProteinModelPortali Q5JKN1.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5JKN1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0552300-01 ; OS01T0552300-01 ; OS01G0552300 . [Q5JKN1-1 ]
GeneIDi 4325851.
KEGGi osa:4325851.

Organism-specific databases

Gramenei Q5JKN1.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000029436.
KOi K04457.
OMAi IVNGRIC.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Nipponbare.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C05_ORYSJ
AccessioniPrimary (citable) accession number: Q5JKN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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