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Protein

Thiamine pyrophosphokinase 1

Gene

TPK1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism (By similarity).By similarity

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (OsJ_01724), Thiamine pyrophosphokinase 3 (TPK3)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 1 (EC:2.7.6.2)
Short name:
OsTPK1
Alternative name(s):
Thiamine kinase 1
Gene namesi
Name:TPK1
Ordered Locus Names:Os01g0931400, LOC_Os01g70580
ORF Names:OSJNBa0052O12.42-1, P0506E04.20-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239681 – 264Thiamine pyrophosphokinase 1Add BLAST264

Proteomic databases

PaxDbiQ5JK24.

Expressioni

Gene expression databases

ExpressionAtlasiQ5JK24. baseline and differential.
GenevisibleiQ5JK24. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g70580.1.

Structurei

3D structure databases

ProteinModelPortaliQ5JK24.
SMRiQ5JK24.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
InParanoidiQ5JK24.
KOiK00949.
OMAiHEIRIER.
OrthoDBiEOG09360JCF.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiView protein in InterPro
IPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR036371. TPK_B1-bd_sf.
IPR007371. TPK_catalytic.
IPR036759. TPK_catalytic_sf.
PfamiView protein in Pfam
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiView protein in SMART
SM00983. TPK_B1_binding. 1 hit.
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5JK24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPTMTHSS SFLRLPATSS PHPPPADDAS AAYAVVVLNQ RLPRFAPLLW
60 70 80 90 100
DRARLRVCAD GGANRVFDGM PELLPAEDPD QVRMRYKPDV IKGDMDSIRP
110 120 130 140 150
EVKEYYSNLG AEIVDESHDQ DTTDLHKCVS FITRNPPGSE ESNLYILVLG
160 170 180 190 200
ALGGRFDHEM GNINVLYRFS NIRIVLLSDD CSIFLLPKTH SHEIHIERSI
210 220 230 240 250
EGPHCGLIPM GSPSASTTTT GLRWNLDNTS MSYGGLISTS NIVEEETVRI
260
TSDSDLIWTI SLRN
Length:264
Mass (Da):29,288
Last modified:February 15, 2005 - v1
Checksum:iD5D19BD6102E2A07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003272 Genomic DNA. Translation: BAD87327.1.
AP004330 Genomic DNA. Translation: BAD88183.1.
AP008207 Genomic DNA. Translation: BAF07211.1.
AP014957 Genomic DNA. Translation: BAS76043.1.
AK105048 mRNA. Translation: BAG97081.1.
RefSeqiXP_015621775.1. XM_015766289.1.
UniGeneiOs.52120.

Genome annotation databases

EnsemblPlantsiOS01T0931400-02; OS01T0931400-02; OS01G0931400.
GeneIDi4326818.
GrameneiOS01T0931400-02; OS01T0931400-02; OS01G0931400.
KEGGiosa:4326818.

Similar proteinsi

Entry informationi

Entry nameiTPK1_ORYSJ
AccessioniPrimary (citable) accession number: Q5JK24
Secondary accession number(s): A0A0P0VCI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: February 15, 2005
Last modified: October 25, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families