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Q5JJY4

- P2C04_ORYSJ

UniProt

Q5JJY4 - P2C04_ORYSJ

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Protein

Protein kinase and PP2C-like domain-containing protein

Gene

Os01g0541900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591ATPPROSITE-ProRule annotation
Active sitei156 – 1561Proton acceptor; for kinase activityPROSITE-ProRule annotation
Metal bindingi426 – 4261Manganese 1By similarity
Metal bindingi426 – 4261Manganese 2By similarity
Metal bindingi427 – 4271Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi598 – 5981Manganese 2By similarity
Metal bindingi638 – 6381Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase and PP2C-like domain-containing protein
Including the following 2 domains:
Probable serine/threonine-protein kinase Os01g0541900 (EC:2.7.11.1)
Probable protein phosphatase 2C 4 (EC:3.1.3.16)
Short name:
OsPP2C04
Gene namesi
Ordered Locus Names:Os01g0541900, LOC_Os01g36080
ORF Names:OSJNBa0062A24.20
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ5JJY4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Protein kinase and PP2C-like domain-containing proteinPRO_0000363250Add
BLAST

Proteomic databases

PRIDEiQ5JJY4.

Expressioni

Gene expression databases

ExpressionAtlasiQ5JJY4. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os01g36080.1.

Structurei

3D structure databases

ProteinModelPortaliQ5JJY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 327296Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini389 – 640252PP2C-likeAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Curated
In the C-terminal section; belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ5JJY4.
OMAiVPYTDLR.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5JJY4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR
60 70 80 90 100
LGGERVAAKK PVLSTSDDLD KFHYQLQLLW WVLPIELDHP GLARLVAAHA
110 120 130 140 150
RPPNYLMFFD FFEPPNLADK IHVEEWNPSV QQVVTIATDL AKALQYLNIL
160 170 180 190 200
GIVHRDIKPA NILIDKDFHP HLADFGLAMY QKDIKHVSVE NWRSSGKPTG
210 220 230 240 250
GFHKKNMVGT LIYMAPEILR KDIHTEKSDV YSFAISINEL LTGVVPYTDL
260 270 280 290 300
RAEAQAHTVL EMTYTEQQLT AAIVSQGLRP ALALPESGAP PSLLSLIQRC
310 320 330 340 350
WDSDPQQRPS FKDITEELKI IEKHIAVNSC SLASPANKSQ NGNTEVHHYQ
360 370 380 390 400
EALSWLNQGE LFAKGNKLDS TVDHWSDIFD QSSKYCPTLS WGSFATCGRR
410 420 430 440 450
ETMEDTHFML PHMSEEKDLH AFGIFDGHRG SAAAEFSVRA VPGFLKQFNS
460 470 480 490 500
NTSPTDALTE AFVRTDIAFR EELILHQKSK RITQKNWHPG CTAVTALIVR
510 520 530 540 550
NKLFVANAGD CRAILNRAGE PFPMTRDHVA SCPKERERIV KEGTEVKWQI
560 570 580 590 600
DTWRVGAAAL QVTRSIGDDD LKPAVTAQPE VIETILSPDD EFLVMASDGL
610 620 630 640 650
WDVMSNEDVL SIIKDTVKEP GMCSKRLATE AAARGSKDNI TVIVVFLRPV

STAERIY
Length:657
Mass (Da):73,191
Last modified:February 15, 2005 - v1
Checksum:iCA595BF1BA9A1202
GO
Isoform 2 (identifier: Q5JJY4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     80-85: WWVLPI → C

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:652
Mass (Da):72,500
Checksum:iF3709E2AF2FD0F2D
GO
Isoform 3 (identifier: Q5JJY4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     527-554: DHVASCPKERERIVKEGTEVKWQIDTWR → VRIIFLLYFARFLKFKHYNIRYVFILPW
     555-657: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:554
Mass (Da):62,413
Checksum:i6D1B9D5C0AB53C0A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti442 – 4421P → H in AK069784. (PubMed:12869764)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei80 – 856WWVLPI → C in isoform 2. CuratedVSP_036255
Alternative sequencei527 – 55428DHVAS…IDTWR → VRIIFLLYFARFLKFKHYNI RYVFILPW in isoform 3. CuratedVSP_036256Add
BLAST
Alternative sequencei555 – 657103Missing in isoform 3. CuratedVSP_036257Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004361 Genomic DNA. Translation: BAD88224.1.
AP008207 Genomic DNA. Translation: BAF05185.1.
AK069784 mRNA. No translation available.
RefSeqiNP_001043271.1. NM_001049806.1. [Q5JJY4-1]
UniGeneiOs.21164.

Genome annotation databases

EnsemblPlantsiOS01T0541900-01; OS01T0541900-01; OS01G0541900. [Q5JJY4-1]
GeneIDi4326869.
KEGGiosa:4326869.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004361 Genomic DNA. Translation: BAD88224.1 .
AP008207 Genomic DNA. Translation: BAF05185.1 .
AK069784 mRNA. No translation available.
RefSeqi NP_001043271.1. NM_001049806.1. [Q5JJY4-1 ]
UniGenei Os.21164.

3D structure databases

ProteinModelPortali Q5JJY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os01g36080.1.

Proteomic databases

PRIDEi Q5JJY4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0541900-01 ; OS01T0541900-01 ; OS01G0541900 . [Q5JJY4-1 ]
GeneIDi 4326869.
KEGGi osa:4326869.

Organism-specific databases

Gramenei Q5JJY4.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi Q5JJY4.
OMAi VPYTDLR.

Gene expression databases

ExpressionAtlasi Q5JJY4. baseline.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Nipponbare.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C04_ORYSJ
AccessioniPrimary (citable) accession number: Q5JJY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 15, 2005
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3