Q5JJY4 (P2C04_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase and PP2C-like domain-containing protein

Including the following 2 domains:

1. Probable serine/threonine-protein kinase Os01g0541900
   EC=2.7.11.1
2. Probable protein phosphatase 2C 4
   Short name=OsPP2C04
   EC=3.1.3.16

Gene names

Ordered Locus Names:	Os01g0541900, LOC_Os01g36080
ORF Names:	OSJNBa0062A24.20

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family. In the C-terminal section; belongs to the PP2C family. Contains 1 PP2C-like domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5JJY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5JJY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     80-85: WWVLPI → C

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Isoform 3 (identifier: Q5JJY4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     527-554: DHVASCPKERERIVKEGTEVKWQIDTWR → VRIIFLLYFARFLKFKHYNIRYVFILPW
     555-657: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Protein kinase and PP2C-like domain-containing protein		PRO_0000363250
Regions
	Domain	32 – 327	296	Protein kinase
	Domain	389 – 640	252	PP2C-like
	Nucleotide binding	38 – 46	9	ATP By similarity
Sites
	Active site	156	1	Proton acceptor; for kinase activity By similarity
	Metal binding	426	1	Manganese 1 By similarity
	Metal binding	426	1	Manganese 2 By similarity
	Metal binding	427	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	598	1	Manganese 2 By similarity
	Metal binding	638	1	Manganese 2 By similarity
	Binding site	59	1	ATP By similarity
Natural variations
	Alternative sequence	80 – 85	6	WWVLPI → C in isoform 2.		VSP_036255
	Alternative sequence	527 – 554	28	DHVAS…IDTWR → VRIIFLLYFARFLKFKHYNI RYVFILPW in isoform 3.		VSP_036256
	Alternative sequence	555 – 657	103	Missing in isoform 3.		VSP_036257
Experimental info
	Sequence conflict	442	1	P → H in AK069784. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: CA595BF1BA9A1202	FASTA	657	73,191
	10 20 30 40 50 60 MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR LGGERVAAKK 70 80 90 100 110 120 PVLSTSDDLD KFHYQLQLLW WVLPIELDHP GLARLVAAHA RPPNYLMFFD FFEPPNLADK 130 140 150 160 170 180 IHVEEWNPSV QQVVTIATDL AKALQYLNIL GIVHRDIKPA NILIDKDFHP HLADFGLAMY 190 200 210 220 230 240 QKDIKHVSVE NWRSSGKPTG GFHKKNMVGT LIYMAPEILR KDIHTEKSDV YSFAISINEL 250 260 270 280 290 300 LTGVVPYTDL RAEAQAHTVL EMTYTEQQLT AAIVSQGLRP ALALPESGAP PSLLSLIQRC 310 320 330 340 350 360 WDSDPQQRPS FKDITEELKI IEKHIAVNSC SLASPANKSQ NGNTEVHHYQ EALSWLNQGE 370 380 390 400 410 420 LFAKGNKLDS TVDHWSDIFD QSSKYCPTLS WGSFATCGRR ETMEDTHFML PHMSEEKDLH 430 440 450 460 470 480 AFGIFDGHRG SAAAEFSVRA VPGFLKQFNS NTSPTDALTE AFVRTDIAFR EELILHQKSK 490 500 510 520 530 540 RITQKNWHPG CTAVTALIVR NKLFVANAGD CRAILNRAGE PFPMTRDHVA SCPKERERIV 550 560 570 580 590 600 KEGTEVKWQI DTWRVGAAAL QVTRSIGDDD LKPAVTAQPE VIETILSPDD EFLVMASDGL 610 620 630 640 650 WDVMSNEDVL SIIKDTVKEP GMCSKRLATE AAARGSKDNI TVIVVFLRPV STAERIY
	Isoform 2 [UniParc]. Checksum: F3709E2AF2FD0F2D	FASTA	652	72,500
	10 20 30 40 50 60 MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR LGGERVAAKK 70 80 90 100 110 120 PVLSTSDDLD KFHYQLQLLC ELDHPGLARL VAAHARPPNY LMFFDFFEPP NLADKIHVEE 130 140 150 160 170 180 WNPSVQQVVT IATDLAKALQ YLNILGIVHR DIKPANILID KDFHPHLADF GLAMYQKDIK 190 200 210 220 230 240 HVSVENWRSS GKPTGGFHKK NMVGTLIYMA PEILRKDIHT EKSDVYSFAI SINELLTGVV 250 260 270 280 290 300 PYTDLRAEAQ AHTVLEMTYT EQQLTAAIVS QGLRPALALP ESGAPPSLLS LIQRCWDSDP 310 320 330 340 350 360 QQRPSFKDIT EELKIIEKHI AVNSCSLASP ANKSQNGNTE VHHYQEALSW LNQGELFAKG 370 380 390 400 410 420 NKLDSTVDHW SDIFDQSSKY CPTLSWGSFA TCGRRETMED THFMLPHMSE EKDLHAFGIF 430 440 450 460 470 480 DGHRGSAAAE FSVRAVPGFL KQFNSNTSPT DALTEAFVRT DIAFREELIL HQKSKRITQK 490 500 510 520 530 540 NWHPGCTAVT ALIVRNKLFV ANAGDCRAIL NRAGEPFPMT RDHVASCPKE RERIVKEGTE 550 560 570 580 590 600 VKWQIDTWRV GAAALQVTRS IGDDDLKPAV TAQPEVIETI LSPDDEFLVM ASDGLWDVMS 610 620 630 640 650 NEDVLSIIKD TVKEPGMCSK RLATEAAARG SKDNITVIVV FLRPVSTAER IY
	Isoform 3 [UniParc]. Checksum: 6D1B9D5C0AB53C0A	FASTA	554	62,413
	10 20 30 40 50 60 MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR LGGERVAAKK 70 80 90 100 110 120 PVLSTSDDLD KFHYQLQLLW WVLPIELDHP GLARLVAAHA RPPNYLMFFD FFEPPNLADK 130 140 150 160 170 180 IHVEEWNPSV QQVVTIATDL AKALQYLNIL GIVHRDIKPA NILIDKDFHP HLADFGLAMY 190 200 210 220 230 240 QKDIKHVSVE NWRSSGKPTG GFHKKNMVGT LIYMAPEILR KDIHTEKSDV YSFAISINEL 250 260 270 280 290 300 LTGVVPYTDL RAEAQAHTVL EMTYTEQQLT AAIVSQGLRP ALALPESGAP PSLLSLIQRC 310 320 330 340 350 360 WDSDPQQRPS FKDITEELKI IEKHIAVNSC SLASPANKSQ NGNTEVHHYQ EALSWLNQGE 370 380 390 400 410 420 LFAKGNKLDS TVDHWSDIFD QSSKYCPTLS WGSFATCGRR ETMEDTHFML PHMSEEKDLH 430 440 450 460 470 480 AFGIFDGHRG SAAAEFSVRA VPGFLKQFNS NTSPTDALTE AFVRTDIAFR EELILHQKSK 490 500 510 520 530 540 RITQKNWHPG CTAVTALIVR NKLFVANAGD CRAILNRAGE PFPMTRVRII FLLYFARFLK 550 FKHYNIRYVF ILPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Nipponbare.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004361 Genomic DNA. Translation: BAD88224.1. AP008207 Genomic DNA. Translation: BAF05185.1. AK069784 mRNA. No translation available.
RefSeq	NP_001043271.1. NM_001049806.1.
UniGene	Os.21164.
3D structure databases
ProteinModelPortal	Q5JJY4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4326869.
KEGG	osa:4326869.
Organism-specific databases
Gramene	Q5JJY4.
Phylogenomic databases
eggNOG	COG0515.
OMA	NWRSSGK.
ProtClustDB	CLSN2680164.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR001932. PP2C-like. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C04_ORYSJ

Accession

Primary (citable) accession number: Q5JJY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program