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Q5JJY4

- P2C04_ORYSJ

UniProt

Q5JJY4 - P2C04_ORYSJ

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Protein
Protein kinase and PP2C-like domain-containing protein
Gene
Os01g0541900, LOC_Os01g36080, OSJNBa0062A24.20
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591ATP By similarity
Active sitei156 – 1561Proton acceptor; for kinase activity By similarity
Metal bindingi426 – 4261Manganese 1 By similarity
Metal bindingi426 – 4261Manganese 2 By similarity
Metal bindingi427 – 4271Manganese 1; via carbonyl oxygen By similarity
Metal bindingi598 – 5981Manganese 2 By similarity
Metal bindingi638 – 6381Manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Kinase, Protein phosphatase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase and PP2C-like domain-containing protein
    Including the following 2 domains:
    Probable serine/threonine-protein kinase Os01g0541900 (EC:2.7.11.1)
    Probable protein phosphatase 2C 4 (EC:3.1.3.16)
    Short name:
    OsPP2C04
    Gene namesi
    Ordered Locus Names:Os01g0541900, LOC_Os01g36080
    ORF Names:OSJNBa0062A24.20
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ5JJY4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Protein kinase and PP2C-like domain-containing protein
    PRO_0000363250Add
    BLAST

    Proteomic databases

    PRIDEiQ5JJY4.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os01g36080.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JJY4.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 327296Protein kinase
    Add
    BLAST
    Domaini389 – 640252PP2C-like
    Add
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    In the C-terminal section; belongs to the PP2C family.
    Contains 1 PP2C-like domain.

    Phylogenomic databases

    eggNOGiCOG0515.
    OMAiVPYTDLR.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR001932. PP2C-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5JJY4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR    50
    LGGERVAAKK PVLSTSDDLD KFHYQLQLLW WVLPIELDHP GLARLVAAHA 100
    RPPNYLMFFD FFEPPNLADK IHVEEWNPSV QQVVTIATDL AKALQYLNIL 150
    GIVHRDIKPA NILIDKDFHP HLADFGLAMY QKDIKHVSVE NWRSSGKPTG 200
    GFHKKNMVGT LIYMAPEILR KDIHTEKSDV YSFAISINEL LTGVVPYTDL 250
    RAEAQAHTVL EMTYTEQQLT AAIVSQGLRP ALALPESGAP PSLLSLIQRC 300
    WDSDPQQRPS FKDITEELKI IEKHIAVNSC SLASPANKSQ NGNTEVHHYQ 350
    EALSWLNQGE LFAKGNKLDS TVDHWSDIFD QSSKYCPTLS WGSFATCGRR 400
    ETMEDTHFML PHMSEEKDLH AFGIFDGHRG SAAAEFSVRA VPGFLKQFNS 450
    NTSPTDALTE AFVRTDIAFR EELILHQKSK RITQKNWHPG CTAVTALIVR 500
    NKLFVANAGD CRAILNRAGE PFPMTRDHVA SCPKERERIV KEGTEVKWQI 550
    DTWRVGAAAL QVTRSIGDDD LKPAVTAQPE VIETILSPDD EFLVMASDGL 600
    WDVMSNEDVL SIIKDTVKEP GMCSKRLATE AAARGSKDNI TVIVVFLRPV 650
    STAERIY 657
    Length:657
    Mass (Da):73,191
    Last modified:February 15, 2005 - v1
    Checksum:iCA595BF1BA9A1202
    GO
    Isoform 2 (identifier: Q5JJY4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         80-85: WWVLPI → C

    Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

    Show »
    Length:652
    Mass (Da):72,500
    Checksum:iF3709E2AF2FD0F2D
    GO
    Isoform 3 (identifier: Q5JJY4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         527-554: DHVASCPKERERIVKEGTEVKWQIDTWR → VRIIFLLYFARFLKFKHYNIRYVFILPW
         555-657: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:554
    Mass (Da):62,413
    Checksum:i6D1B9D5C0AB53C0A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei80 – 856WWVLPI → C in isoform 2.
    VSP_036255
    Alternative sequencei527 – 55428DHVAS…IDTWR → VRIIFLLYFARFLKFKHYNI RYVFILPW in isoform 3.
    VSP_036256Add
    BLAST
    Alternative sequencei555 – 657103Missing in isoform 3.
    VSP_036257Add
    BLAST

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti442 – 4421P → H in AK069784. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004361 Genomic DNA. Translation: BAD88224.1.
    AP008207 Genomic DNA. Translation: BAF05185.1.
    AK069784 mRNA. No translation available.
    RefSeqiNP_001043271.1. NM_001049806.1. [Q5JJY4-1]
    UniGeneiOs.21164.

    Genome annotation databases

    EnsemblPlantsiOS01T0541900-01; OS01T0541900-01; OS01G0541900. [Q5JJY4-1]
    GeneIDi4326869.
    KEGGiosa:4326869.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP004361 Genomic DNA. Translation: BAD88224.1 .
    AP008207 Genomic DNA. Translation: BAF05185.1 .
    AK069784 mRNA. No translation available.
    RefSeqi NP_001043271.1. NM_001049806.1. [Q5JJY4-1 ]
    UniGenei Os.21164.

    3D structure databases

    ProteinModelPortali Q5JJY4.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os01g36080.1.

    Proteomic databases

    PRIDEi Q5JJY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0541900-01 ; OS01T0541900-01 ; OS01G0541900 . [Q5JJY4-1 ]
    GeneIDi 4326869.
    KEGGi osa:4326869.

    Organism-specific databases

    Gramenei Q5JJY4.

    Phylogenomic databases

    eggNOGi COG0515.
    OMAi VPYTDLR.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR001932. PP2C-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Nipponbare.
    5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C04_ORYSJ
    AccessioniPrimary (citable) accession number: Q5JJY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: February 15, 2005
    Last modified: May 14, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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