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Q5JJY4 (P2C04_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase and PP2C-like domain-containing protein

Including the following 2 domains:

  1. Probable serine/threonine-protein kinase Os01g0541900
    EC=2.7.11.1
  2. Probable protein phosphatase 2C 4
    Short name=OsPP2C04
    EC=3.1.3.16
Gene names
Ordered Locus Names:Os01g0541900, LOC_Os01g36080
ORF Names:OSJNBa0062A24.20
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Sequence similarities

In the N-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

In the C-terminal section; belongs to the PP2C family.

Contains 1 PP2C-like domain.

Contains 1 protein kinase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5JJY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5JJY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     80-85: WWVLPI → C
Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.
Isoform 3 (identifier: Q5JJY4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     527-554: DHVASCPKERERIVKEGTEVKWQIDTWR → VRIIFLLYFARFLKFKHYNIRYVFILPW
     555-657: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657Protein kinase and PP2C-like domain-containing protein
PRO_0000363250

Regions

Domain32 – 327296Protein kinase
Domain389 – 640252PP2C-like
Nucleotide binding38 – 469ATP By similarity

Sites

Active site1561Proton acceptor; for kinase activity By similarity
Metal binding4261Manganese 1 By similarity
Metal binding4261Manganese 2 By similarity
Metal binding4271Manganese 1; via carbonyl oxygen By similarity
Metal binding5981Manganese 2 By similarity
Metal binding6381Manganese 2 By similarity
Binding site591ATP By similarity

Natural variations

Alternative sequence80 – 856WWVLPI → C in isoform 2.
VSP_036255
Alternative sequence527 – 55428DHVAS…IDTWR → VRIIFLLYFARFLKFKHYNI RYVFILPW in isoform 3.
VSP_036256
Alternative sequence555 – 657103Missing in isoform 3.
VSP_036257

Experimental info

Sequence conflict4421P → H in AK069784. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: CA595BF1BA9A1202

FASTA65773,191
        10         20         30         40         50         60 
MGVEVPPEES NRCVRGCCRS AAIPLHLPPS SFSLLSPIAK GSESTVYEAR LGGERVAAKK 

        70         80         90        100        110        120 
PVLSTSDDLD KFHYQLQLLW WVLPIELDHP GLARLVAAHA RPPNYLMFFD FFEPPNLADK 

       130        140        150        160        170        180 
IHVEEWNPSV QQVVTIATDL AKALQYLNIL GIVHRDIKPA NILIDKDFHP HLADFGLAMY 

       190        200        210        220        230        240 
QKDIKHVSVE NWRSSGKPTG GFHKKNMVGT LIYMAPEILR KDIHTEKSDV YSFAISINEL 

       250        260        270        280        290        300 
LTGVVPYTDL RAEAQAHTVL EMTYTEQQLT AAIVSQGLRP ALALPESGAP PSLLSLIQRC 

       310        320        330        340        350        360 
WDSDPQQRPS FKDITEELKI IEKHIAVNSC SLASPANKSQ NGNTEVHHYQ EALSWLNQGE 

       370        380        390        400        410        420 
LFAKGNKLDS TVDHWSDIFD QSSKYCPTLS WGSFATCGRR ETMEDTHFML PHMSEEKDLH 

       430        440        450        460        470        480 
AFGIFDGHRG SAAAEFSVRA VPGFLKQFNS NTSPTDALTE AFVRTDIAFR EELILHQKSK 

       490        500        510        520        530        540 
RITQKNWHPG CTAVTALIVR NKLFVANAGD CRAILNRAGE PFPMTRDHVA SCPKERERIV 

       550        560        570        580        590        600 
KEGTEVKWQI DTWRVGAAAL QVTRSIGDDD LKPAVTAQPE VIETILSPDD EFLVMASDGL 

       610        620        630        640        650 
WDVMSNEDVL SIIKDTVKEP GMCSKRLATE AAARGSKDNI TVIVVFLRPV STAERIY 

« Hide

Isoform 2 [UniParc].

Checksum: F3709E2AF2FD0F2D
Show »

FASTA65272,500
Isoform 3 [UniParc].

Checksum: 6D1B9D5C0AB53C0A
Show »

FASTA55462,413

References

« Hide 'large scale' references
[1]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Nipponbare.
[5]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP004361 Genomic DNA. Translation: BAD88224.1.
AP008207 Genomic DNA. Translation: BAF05185.1.
AK069784 mRNA. No translation available.
RefSeqNP_001043271.1. NM_001049806.1.
UniGeneOs.21164.

3D structure databases

ProteinModelPortalQ5JJY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os01g36080.1.

Proteomic databases

PRIDEQ5JJY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0541900-01; OS01T0541900-01; OS01G0541900. [Q5JJY4-1]
GeneID4326869.
KEGGosa:4326869.

Organism-specific databases

GrameneQ5JJY4.

Phylogenomic databases

eggNOGCOG0515.
OMAVPYTDLR.
ProtClustDBCLSN2680164.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR001932. PP2C-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP2C04_ORYSJ
AccessionPrimary (citable) accession number: Q5JJY4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations