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Q5JJV0

- INV4_ORYSJ

UniProt

Q5JJV0 - INV4_ORYSJ

Protein

Beta-fructofuranosidase, insoluble isoenzyme 4

Gene

CIN4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    May play a role in sucrose partitioning during seed development and in stress response.

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei74 – 741PROSITE-ProRule annotation
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei98 – 981SubstrateBy similarity
    Binding sitei253 – 2531SubstrateBy similarity
    Binding sitei287 – 2871SubstrateBy similarity

    GO - Molecular functioni

    1. sucrose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH32. Glycoside Hydrolase Family 32.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-fructofuranosidase, insoluble isoenzyme 4 (EC:3.2.1.26)
    Alternative name(s):
    Cell wall beta-fructosidase 4
    Invertase 4
    OsCIN4
    Sucrose hydrolase 4
    Gene namesi
    Name:CIN4
    Ordered Locus Names:Os01g0966700, LOC_Os01g73580
    ORF Names:P0458E05.32
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ5JJV0.

    Subcellular locationi

    Secretedextracellular spaceapoplast Curated. Secretedcell wall Curated
    Note: Associated to the cell wall.Curated

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 590562Beta-fructofuranosidase, insoluble isoenzyme 4PRO_0000033382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi445 ↔ 491By similarity
    Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ5JJV0.

    Expressioni

    Tissue specificityi

    Expressed in leaves. Expressed at moderate levels in roots and flowers, and weakly in seeds.1 Publication

    Developmental stagei

    Expressed from 1 to 10 days after flowering.2 Publications

    Inductioni

    By rice blast fungus (M.grisea) 12 hours after infection.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JJV0.
    SMRiQ5JJV0. Positions 57-586.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni71 – 744Substrate bindingBy similarity
    Regioni133 – 1342Substrate bindingBy similarity
    Regioni198 – 1992Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 32 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1621.
    HOGENOMiHOG000181427.
    KOiK01193.
    OMAiWTNETDS.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view]
    SMARTiSM00640. Glyco_32. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5JJV0-1 [UniParc]FASTAAdd to Basket

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    MVMAPIPQPW HQWPFLILFF LVLFSCESNL PCRNGVEATQ RVFLYPQSPK    50
    VSSIVSKGYR TGYHFQPPKN WINDPNGPMY YNGIYHEFYQ YNPNGSVWGN 100
    IVWGHSVSTD LINWIRLEPA IEGNTPSDIN GCWTGSATIL TGDQPVIIYT 150
    GADTEKRQVQ NIVLPKNRSD PYLREWTKPK NNPLIEPVGP GLNSNQFRDP 200
    TTGWIGPDGL WRIAVGAELN GYSAALLYKS KDFMQWTRVD HPLYSSNASN 250
    MWECPDFFAV LPGKNNGLDL SAAIPNGAKH VLKMSLDSCD KYMIGVYDLK 300
    HDMFVPDTVL DDRRLWLRID YGNYYASKSF FDSKKGRRII WGWTNETDST 350
    SDDVAKGWAG IHAIPRTIWL DGDGKRLLQW PIEEVESLRR NEVSHQGLEL 400
    KKGDLFEIKG TDTLQADVEI DFELTSIDAA DPFDPSWLLD TEKHCREADA 450
    SVHGGLGPFG LVVLASDNMD EHTTVHFRVY KSEQKYMVLL CSDLRRSSLR 500
    PGLYTPAYGG FFEYDLEKEK KISLRTLIDR SAVESFGGGG RACIMARVYP 550
    AAVVDGATHM YAFNNGSSTV KVSQLKAWSM TRAQVNVRKG 590
    Length:590
    Mass (Da):66,610
    Last modified:February 15, 2005 - v1
    Checksum:i4999F60849A679E4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti226 – 2261L → P in BAD05180. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY578161 mRNA. Translation: AAT84404.1.
    AB154521 mRNA. Translation: BAD05180.1.
    AP004365 Genomic DNA. Translation: BAD88258.1.
    RefSeqiNP_001045504.1. NM_001052039.1.
    UniGeneiOs.11387.

    Genome annotation databases

    EnsemblPlantsiOS01T0966700-01; OS01T0966700-01; OS01G0966700.
    GeneIDi4324001.
    KEGGiosa:4324001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY578161 mRNA. Translation: AAT84404.1 .
    AB154521 mRNA. Translation: BAD05180.1 .
    AP004365 Genomic DNA. Translation: BAD88258.1 .
    RefSeqi NP_001045504.1. NM_001052039.1.
    UniGenei Os.11387.

    3D structure databases

    ProteinModelPortali Q5JJV0.
    SMRi Q5JJV0. Positions 57-586.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH32. Glycoside Hydrolase Family 32.

    Proteomic databases

    PRIDEi Q5JJV0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS01T0966700-01 ; OS01T0966700-01 ; OS01G0966700 .
    GeneIDi 4324001.
    KEGGi osa:4324001.

    Organism-specific databases

    Gramenei Q5JJV0.

    Phylogenomic databases

    eggNOGi COG1621.
    HOGENOMi HOG000181427.
    KOi K01193.
    OMAi WTNETDS.

    Family and domain databases

    Gene3Di 2.115.10.20. 1 hit.
    2.60.120.560. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR001362. Glyco_hydro_32.
    IPR018053. Glyco_hydro_32_AS.
    IPR013189. Glyco_hydro_32_C.
    IPR013148. Glyco_hydro_32_N.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view ]
    Pfami PF08244. Glyco_hydro_32C. 1 hit.
    PF00251. Glyco_hydro_32N. 1 hit.
    [Graphical view ]
    SMARTi SM00640. Glyco_32. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF75005. SSF75005. 1 hit.
    PROSITEi PS00609. GLYCOSYL_HYDROL_F32. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)."
      Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S.
      Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: cv. Nipponbare.
    2. Hirose T., Terao T.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Panicle.
    3. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle."
      Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H., Terao T., Ishii R., Ohsugi R., Yamagishi T.
      Plant Cell Physiol. 46:620-628(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiINV4_ORYSJ
    AccessioniPrimary (citable) accession number: Q5JJV0
    Secondary accession number(s): Q75SP0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3