Q5JJV0 (INV4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 4
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 4
Invertase 4
OsCIN4
Sucrose hydrolase 4

Gene names

Name:	CIN4
Ordered Locus Names:	Os01g0966700, LOC_Os01g73580
ORF Names:	P0458E05.32

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in sucrose partitioning during seed development and in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in leaves. Expressed at moderate levels in roots and flowers, and weakly in seeds. Ref.1
Developmental stage	Expressed from 1 to 10 days after flowering. Ref.1 Ref.4
Induction	By rice blast fungus (M.grisea) 12 hours after infection. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 590	562	Beta-fructofuranosidase, insoluble isoenzyme 4		PRO_0000033382
Regions
	Region	71 – 74	4	Substrate binding By similarity
	Region	133 – 134	2	Substrate binding By similarity
	Region	198 – 199	2	Substrate binding By similarity
Sites
	Active site	74	1	By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Binding site	287	1	Substrate By similarity
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	565	1	N-linked (GlcNAc...) Potential
	Disulfide bond	445 ↔ 491		By similarity
Experimental info
	Sequence conflict	226	1	L → P in BAD05180. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5JJV0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4999F60849A679E4	FASTA	590	66,610
	10 20 30 40 50 60 MVMAPIPQPW HQWPFLILFF LVLFSCESNL PCRNGVEATQ RVFLYPQSPK VSSIVSKGYR 70 80 90 100 110 120 TGYHFQPPKN WINDPNGPMY YNGIYHEFYQ YNPNGSVWGN IVWGHSVSTD LINWIRLEPA 130 140 150 160 170 180 IEGNTPSDIN GCWTGSATIL TGDQPVIIYT GADTEKRQVQ NIVLPKNRSD PYLREWTKPK 190 200 210 220 230 240 NNPLIEPVGP GLNSNQFRDP TTGWIGPDGL WRIAVGAELN GYSAALLYKS KDFMQWTRVD 250 260 270 280 290 300 HPLYSSNASN MWECPDFFAV LPGKNNGLDL SAAIPNGAKH VLKMSLDSCD KYMIGVYDLK 310 320 330 340 350 360 HDMFVPDTVL DDRRLWLRID YGNYYASKSF FDSKKGRRII WGWTNETDST SDDVAKGWAG 370 380 390 400 410 420 IHAIPRTIWL DGDGKRLLQW PIEEVESLRR NEVSHQGLEL KKGDLFEIKG TDTLQADVEI 430 440 450 460 470 480 DFELTSIDAA DPFDPSWLLD TEKHCREADA SVHGGLGPFG LVVLASDNMD EHTTVHFRVY 490 500 510 520 530 540 KSEQKYMVLL CSDLRRSSLR PGLYTPAYGG FFEYDLEKEK KISLRTLIDR SAVESFGGGG 550 560 570 580 590 RACIMARVYP AAVVDGATHM YAFNNGSSTV KVSQLKAWSM TRAQVNVRKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)." Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S. Plant Cell Rep. 24:225-236(2005) [PubMed: 15759120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Nipponbare.
[2]	Hirose T., Terao T. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Panicle.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle." Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H., Terao T., Ishii R., Ohsugi R., Yamagishi T. Plant Cell Physiol. 46:620-628(2005) [PubMed: 15701658] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY578161 mRNA. Translation: AAT84404.1. AB154521 mRNA. Translation: BAD05180.1. AP004365 Genomic DNA. Translation: BAD88258.1.
RefSeq	NP_001045504.1. NM_001052039.1.
UniGene	Os.11387.
3D structure databases
ProteinModelPortal	Q5JJV0.
SMR	Q5JJV0. Positions 57-586.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5JJV0.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Proteomic databases
PRIDE	Q5JJV0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g73580.1; LOC_Os01g73580.1; LOC_Os01g73580.
GeneID	4324001.
KEGG	osa:4324001.
Organism-specific databases
Gramene	Q5JJV0.
Phylogenomic databases
GeneTree	EPGT00050000007871.
OMA	HAIPRTI.
PhylomeDB	Q5JJV0.
ProtClustDB	CLSN2692328.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV4_ORYSJ

Accession

Primary (citable) accession number: Q5JJV0
Secondary accession number(s): Q75SP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program