Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5JJV0

- INV4_ORYSJ

UniProt

Q5JJV0 - INV4_ORYSJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-fructofuranosidase, insoluble isoenzyme 4

Gene

CIN4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

May play a role in sucrose partitioning during seed development and in stress response.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741PROSITE-ProRule annotation
Binding sitei90 – 901SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei253 – 2531SubstrateBy similarity
Binding sitei287 – 2871SubstrateBy similarity

GO - Molecular functioni

  1. sucrose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 4 (EC:3.2.1.26)
Alternative name(s):
Cell wall beta-fructosidase 4
Invertase 4
OsCIN4
Sucrose hydrolase 4
Gene namesi
Name:CIN4
Ordered Locus Names:Os01g0966700, LOC_Os01g73580
ORF Names:P0458E05.32
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ5JJV0.

Subcellular locationi

Secretedextracellular spaceapoplast Curated. Secretedcell wall Curated
Note: Associated to the cell wall.Curated

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 590562Beta-fructofuranosidase, insoluble isoenzyme 4PRO_0000033382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi445 ↔ 491By similarity
Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5JJV0.

Expressioni

Tissue specificityi

Expressed in leaves. Expressed at moderate levels in roots and flowers, and weakly in seeds.1 Publication

Developmental stagei

Expressed from 1 to 10 days after flowering.2 Publications

Inductioni

By rice blast fungus (M.grisea) 12 hours after infection.1 Publication

Gene expression databases

ExpressionAtlasiQ5JJV0. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ5JJV0.
SMRiQ5JJV0. Positions 57-586.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 744Substrate bindingBy similarity
Regioni133 – 1342Substrate bindingBy similarity
Regioni198 – 1992Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 32 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1621.
HOGENOMiHOG000181427.
InParanoidiQ5JJV0.
KOiK01193.
OMAiWTNETDS.

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamiPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JJV0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVMAPIPQPW HQWPFLILFF LVLFSCESNL PCRNGVEATQ RVFLYPQSPK
60 70 80 90 100
VSSIVSKGYR TGYHFQPPKN WINDPNGPMY YNGIYHEFYQ YNPNGSVWGN
110 120 130 140 150
IVWGHSVSTD LINWIRLEPA IEGNTPSDIN GCWTGSATIL TGDQPVIIYT
160 170 180 190 200
GADTEKRQVQ NIVLPKNRSD PYLREWTKPK NNPLIEPVGP GLNSNQFRDP
210 220 230 240 250
TTGWIGPDGL WRIAVGAELN GYSAALLYKS KDFMQWTRVD HPLYSSNASN
260 270 280 290 300
MWECPDFFAV LPGKNNGLDL SAAIPNGAKH VLKMSLDSCD KYMIGVYDLK
310 320 330 340 350
HDMFVPDTVL DDRRLWLRID YGNYYASKSF FDSKKGRRII WGWTNETDST
360 370 380 390 400
SDDVAKGWAG IHAIPRTIWL DGDGKRLLQW PIEEVESLRR NEVSHQGLEL
410 420 430 440 450
KKGDLFEIKG TDTLQADVEI DFELTSIDAA DPFDPSWLLD TEKHCREADA
460 470 480 490 500
SVHGGLGPFG LVVLASDNMD EHTTVHFRVY KSEQKYMVLL CSDLRRSSLR
510 520 530 540 550
PGLYTPAYGG FFEYDLEKEK KISLRTLIDR SAVESFGGGG RACIMARVYP
560 570 580 590
AAVVDGATHM YAFNNGSSTV KVSQLKAWSM TRAQVNVRKG
Length:590
Mass (Da):66,610
Last modified:February 15, 2005 - v1
Checksum:i4999F60849A679E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261L → P in BAD05180. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY578161 mRNA. Translation: AAT84404.1.
AB154521 mRNA. Translation: BAD05180.1.
AP004365 Genomic DNA. Translation: BAD88258.1.
RefSeqiNP_001045504.1. NM_001052039.1.
UniGeneiOs.11387.

Genome annotation databases

EnsemblPlantsiOS01T0966700-01; OS01T0966700-01; OS01G0966700.
GeneIDi4324001.
KEGGiosa:4324001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY578161 mRNA. Translation: AAT84404.1 .
AB154521 mRNA. Translation: BAD05180.1 .
AP004365 Genomic DNA. Translation: BAD88258.1 .
RefSeqi NP_001045504.1. NM_001052039.1.
UniGenei Os.11387.

3D structure databases

ProteinModelPortali Q5JJV0.
SMRi Q5JJV0. Positions 57-586.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH32. Glycoside Hydrolase Family 32.

Proteomic databases

PRIDEi Q5JJV0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS01T0966700-01 ; OS01T0966700-01 ; OS01G0966700 .
GeneIDi 4324001.
KEGGi osa:4324001.

Organism-specific databases

Gramenei Q5JJV0.

Phylogenomic databases

eggNOGi COG1621.
HOGENOMi HOG000181427.
InParanoidi Q5JJV0.
KOi K01193.
OMAi WTNETDS.

Gene expression databases

ExpressionAtlasi Q5JJV0. baseline.

Family and domain databases

Gene3Di 2.115.10.20. 1 hit.
2.60.120.560. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view ]
Pfami PF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view ]
SMARTi SM00640. Glyco_32. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF75005. SSF75005. 1 hit.
PROSITEi PS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)."
    Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S.
    Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: cv. Nipponbare.
  2. Hirose T., Terao T.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Panicle.
  3. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle."
    Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H., Terao T., Ishii R., Ohsugi R., Yamagishi T.
    Plant Cell Physiol. 46:620-628(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiINV4_ORYSJ
AccessioniPrimary (citable) accession number: Q5JJV0
Secondary accession number(s): Q75SP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3