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Q5JJV0 (INV4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-fructofuranosidase, insoluble isoenzyme 4
EC=3.2.1.26
Alternative name(s):
Cell wall beta-fructosidase 4
Invertase 4
OsCIN4
Sucrose hydrolase 4
Gene names
Name:CIN4
Ordered Locus Names:Os01g0966700, LOC_Os01g73580
ORF Names:P0458E05.32
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in sucrose partitioning during seed development and in stress response.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Subcellular location

Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.

Tissue specificity

Expressed in leaves. Expressed at moderate levels in roots and flowers, and weakly in seeds. Ref.1

Developmental stage

Expressed from 1 to 10 days after flowering. Ref.1 Ref.4

Induction

By rice blast fungus (M.grisea) 12 hours after infection. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
   Cellular componentApoplast
Cell wall
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnectar secretion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to karrikin

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to wounding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

starch metabolic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

vacuole

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functioninulinase activity

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

levanase activity

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

sucrose alpha-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 590562Beta-fructofuranosidase, insoluble isoenzyme 4
PRO_0000033382

Regions

Region71 – 744Substrate binding By similarity
Region133 – 1342Substrate binding By similarity
Region198 – 1992Substrate binding By similarity

Sites

Active site741 By similarity
Binding site901Substrate By similarity
Binding site981Substrate By similarity
Binding site2531Substrate By similarity
Binding site2871Substrate By similarity

Amino acid modifications

Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation2471N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation5651N-linked (GlcNAc...) Potential
Disulfide bond445 ↔ 491 By similarity

Experimental info

Sequence conflict2261L → P in BAD05180. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5JJV0 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 4999F60849A679E4

FASTA59066,610
        10         20         30         40         50         60 
MVMAPIPQPW HQWPFLILFF LVLFSCESNL PCRNGVEATQ RVFLYPQSPK VSSIVSKGYR 

        70         80         90        100        110        120 
TGYHFQPPKN WINDPNGPMY YNGIYHEFYQ YNPNGSVWGN IVWGHSVSTD LINWIRLEPA 

       130        140        150        160        170        180 
IEGNTPSDIN GCWTGSATIL TGDQPVIIYT GADTEKRQVQ NIVLPKNRSD PYLREWTKPK 

       190        200        210        220        230        240 
NNPLIEPVGP GLNSNQFRDP TTGWIGPDGL WRIAVGAELN GYSAALLYKS KDFMQWTRVD 

       250        260        270        280        290        300 
HPLYSSNASN MWECPDFFAV LPGKNNGLDL SAAIPNGAKH VLKMSLDSCD KYMIGVYDLK 

       310        320        330        340        350        360 
HDMFVPDTVL DDRRLWLRID YGNYYASKSF FDSKKGRRII WGWTNETDST SDDVAKGWAG 

       370        380        390        400        410        420 
IHAIPRTIWL DGDGKRLLQW PIEEVESLRR NEVSHQGLEL KKGDLFEIKG TDTLQADVEI 

       430        440        450        460        470        480 
DFELTSIDAA DPFDPSWLLD TEKHCREADA SVHGGLGPFG LVVLASDNMD EHTTVHFRVY 

       490        500        510        520        530        540 
KSEQKYMVLL CSDLRRSSLR PGLYTPAYGG FFEYDLEKEK KISLRTLIDR SAVESFGGGG 

       550        560        570        580        590 
RACIMARVYP AAVVDGATHM YAFNNGSSTV KVSQLKAWSM TRAQVNVRKG

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)."
Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S.
Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Strain: cv. Nipponbare.
[2]Hirose T., Terao T.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Panicle.
[3]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle."
Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H., Terao T., Ishii R., Ohsugi R., Yamagishi T.
Plant Cell Physiol. 46:620-628(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY578161 mRNA. Translation: AAT84404.1.
AB154521 mRNA. Translation: BAD05180.1.
AP004365 Genomic DNA. Translation: BAD88258.1.
RefSeqNP_001045504.1. NM_001052039.1.
UniGeneOs.11387.

3D structure databases

ProteinModelPortalQ5JJV0.
SMRQ5JJV0. Positions 57-586.
ModBaseSearch...

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Proteomic databases

PRIDEQ5JJV0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsLOC_Os01g73580.1; LOC_Os01g73580.1; LOC_Os01g73580.
GeneID4324001.
KEGGdosa:Os01t0966700-01.
osa:4324001.

Organism-specific databases

GrameneQ5JJV0.

Phylogenomic databases

eggNOGCOG1621.
HOGENOMHOG000181427.
KOK01193.
OMAHAIPRTI.
ProtClustDBCLSN2692328.

Gene expression databases

ArrayExpressQ5JJV0.

Family and domain databases

Gene3D2.115.10.20. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameINV4_ORYSJ
AccessionPrimary (citable) accession number: Q5JJV0
Secondary accession number(s): Q75SP0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

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