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Q5JJB8

- PNPH_THEKO

UniProt

Q5JJB8 - PNPH_THEKO

Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

TK1482

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101PhosphateUniRule annotation
    Sitei170 – 1701Important for substrate specificityUniRule annotation
    Binding sitei188 – 1881Substrate; via amide nitrogenUniRule annotation
    Binding sitei189 – 1891PhosphateUniRule annotation
    Sitei224 – 2241Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1509-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Alternative name(s):
    Purine nucleoside phosphorylaseUniRule annotation
    Short name:
    PNPUniRule annotation
    Gene namesi
    Ordered Locus Names:TK1482
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Probable 6-oxopurine nucleoside phosphorylasePRO_0000415087Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    IntActiQ5JJB8. 1 interaction.
    MINTiMINT-8377038.
    STRINGi69014.TK1482.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JJB8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 512Phosphate bindingUniRule annotation
    Regioni83 – 842Phosphate bindingUniRule annotation
    Regioni212 – 2143Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiCPELRKA.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5JJB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRIAIIGGS GVYDPALLTN IREETVETPY GTVKVKIGEY NGEEIVFLAR    50
    HGEGHSVPPH KINYRANIWA LYELGVERIL STSAVGSLNL DMKPGDFVIL 100
    DQLMDFTKTR HYTFYDGEES PHDRKFVAHV DFTEPYCPEL RKALMNAARE 150
    LGFTYHPMGT YACMEGPRFE TRAEIRALKI LGADVVGMTQ CPEAALAREL 200
    EMCYASVAIV TNYAAGISPN KLTHTEVVEL MAQKSNEIKL LLMKAIEYIP 250
    KERRCPCKDA LKGATGE 267
    Length:267
    Mass (Da):29,804
    Last modified:February 15, 2005 - v1
    Checksum:i2E1BA87711CB899B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD85671.1.
    RefSeqiYP_183895.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD85671; BAD85671; TK1482.
    GeneIDi3234499.
    KEGGitko:TK1482.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD85671.1 .
    RefSeqi YP_183895.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q5JJB8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q5JJB8. 1 interaction.
    MINTi MINT-8377038.
    STRINGi 69014.TK1482.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD85671 ; BAD85671 ; TK1482 .
    GeneIDi 3234499.
    KEGGi tko:TK1482.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi CPELRKA.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci TKOD69014:GH72-1509-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiPNPH_THEKO
    AccessioniPrimary (citable) accession number: Q5JJB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3