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Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

TK1482

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101PhosphateUniRule annotation
Sitei170 – 1701Important for substrate specificityUniRule annotation
Binding sitei188 – 1881Substrate; via amide nitrogenUniRule annotation
Binding sitei189 – 1891PhosphateUniRule annotation
Sitei224 – 2241Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
  3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1509-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Alternative name(s):
Purine nucleoside phosphorylaseUniRule annotation
Short name:
PNPUniRule annotation
Gene namesi
Ordered Locus Names:TK1482
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Probable 6-oxopurine nucleoside phosphorylasePRO_0000415087Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

IntActiQ5JJB8. 1 interaction.
MINTiMINT-8377038.
STRINGi69014.TK1482.

Structurei

3D structure databases

ProteinModelPortaliQ5JJB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Phosphate bindingUniRule annotation
Regioni83 – 842Phosphate bindingUniRule annotation
Regioni212 – 2143Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiQ5JJB8.
KOiK00772.
OMAiMEGPRFE.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JJB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRIAIIGGS GVYDPALLTN IREETVETPY GTVKVKIGEY NGEEIVFLAR
60 70 80 90 100
HGEGHSVPPH KINYRANIWA LYELGVERIL STSAVGSLNL DMKPGDFVIL
110 120 130 140 150
DQLMDFTKTR HYTFYDGEES PHDRKFVAHV DFTEPYCPEL RKALMNAARE
160 170 180 190 200
LGFTYHPMGT YACMEGPRFE TRAEIRALKI LGADVVGMTQ CPEAALAREL
210 220 230 240 250
EMCYASVAIV TNYAAGISPN KLTHTEVVEL MAQKSNEIKL LLMKAIEYIP
260
KERRCPCKDA LKGATGE
Length:267
Mass (Da):29,804
Last modified:February 15, 2005 - v1
Checksum:i2E1BA87711CB899B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85671.1.
RefSeqiYP_183895.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85671; BAD85671; TK1482.
GeneIDi3234499.
KEGGitko:TK1482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85671.1.
RefSeqiYP_183895.1. NC_006624.1.

3D structure databases

ProteinModelPortaliQ5JJB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5JJB8. 1 interaction.
MINTiMINT-8377038.
STRINGi69014.TK1482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85671; BAD85671; TK1482.
GeneIDi3234499.
KEGGitko:TK1482.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiQ5JJB8.
KOiK00772.
OMAiMEGPRFE.

Enzyme and pathway databases

UniPathwayiUPA00606.
BioCyciTKOD69014:GH72-1509-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiPNPH_THEKO
AccessioniPrimary (citable) accession number: Q5JJB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: February 15, 2005
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.