Q5JJ65 (NADE_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	TK1798

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152233
Regions
	Nucleotide binding	32 – 39	8	ATP By similarity
Sites
	Active site	34	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JJ65 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: B106C7C55E281B68	FASTA	254	28,366
	10 20 30 40 50 60 MRALDYRAAI ETITSFLSEK LEESGSGGFV IGISGGIDSA TAAYLAAKAV GTENVLGLIM 70 80 90 100 110 120 PYYENNDVED AKLVCESLGI DYEVINIKPI VESFVSQLGF QPDKRSLGNI MSRTRMILLY 130 140 150 160 170 180 AHANQLNRLV LGTSNRSEFL TGYFTKWGDG ASDYAPLINL YKTEVWEIAK LLGVPGRIIQ 190 200 210 220 230 240 KKPTAGLWEG QTDEDELGIS YRLLDEILWR LVDLKMPKEK IVEELGISVE KVEYVEGLVR 250 RSEHKRRLPV GPEF

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85987.1.
RefSeq	YP_184211.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JJ65.
SMR	Q5JJ65. Positions 1-254.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006549; EBPYRP00000006330; EBPYRG00000006548.
GeneID	3235566.
GenomeReviews	Gene locus TK1798 in contig AP006878_GR.
KEGG	tko:TK1798.
NMPDR	fig|69014.3.peg.1648.
Phylogenomic databases
eggNOG	arNOG08797.
GeneTree	EBGT00050000022961.
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	Q5JJ65.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1798-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_PYRKO

Accession

Primary (citable) accession number: Q5JJ65

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program