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Q5JJ31

- SYI_THEKO

UniProt

Q5JJ31 - SYI_THEKO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1784-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TK1748
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065Isoleucine--tRNA ligasePRO_0000098591Add
BLAST

Proteomic databases

PRIDEiQ5JJ31.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1748.

Structurei

3D structure databases

ProteinModelPortaliQ5JJ31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ5JJ31.
KOiK01870.
OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JJ31-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIREPEFREY NPGQLEEKIE AFWKENNTYE KVKKLRENGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRSMQGYNV RRQPGFDMHG LPIEVKVEQA
110 120 130 140 150
LKLKNKREIE TKIGVDNFIK KCKEFALNNL KIMTEQFKQL GVWMDWDNPY
160 170 180 190 200
MTIKNEYIES GWFTLKRAWE KGLLEKDKRV LHWCPRCQTA LAEHEVRGEY
210 220 230 240 250
KMRKDPSIYV KFPIEWKEKE YLLIWTTTPW TLPANLAVAA HPEYEYAKVK
260 270 280 290 300
VETENGEEYW IMAKALVERV LSEVGVKGEI VETFKGEELE GIRYTHVLLE
310 320 330 340 350
EYPAQKEFRE KYEWAHRVIL GEHVTLEDGT GLVHTAPGHG EEDFEVGQRY
360 370 380 390 400
GLPVYSPVDD AGRYTEGKWK GVYVKDADPE IIEYLKEKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKVSKVKDQI IKENDEKVTW YPEWVKVRYD
460 470 480 490 500
NGVMNSGDWV ISRQRYWGIP LPIWESEDGE YYVVGSFEEL VKLAVAIEVN
510 520 530 540 550
GERIDLPEGY EEKLKIIEEK LGPEDLHRPY VDAFIIKVNG KEMKRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLDYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
VIAFDTVPYR AVAMHGYVLD EKGDKMSKSL GNIIRPEEVV QKEGRDPFRF
660 670 680 690 700
YMLWATNPWE NLRFSWKGLE QVKRMLNILW NVYVLSATYM SLDNFNPTKV
710 720 730 740 750
NPEELPFREE DRWILSRVNS LIGEVTDGIE TFRLTRATRG IYNFVVEDLS
760 770 780 790 800
RWYIRLIRKR MWVEGDDPDK LAAYYTVWKV FDVLLRLMAP FTPYIAEEIY
810 820 830 840 850
QNLIRPFVGV ESVHMLDWPE ADEKAIDEEL EKEMEYARKI VEAGSSARQK
860 870 880 890 900
ARIKLRYPVR RIIIETEDET VKKAVERINR ILRDQLNAKE VVVGKVEREL
910 920 930 940 950
IIKPNFAKVG PEFKGDAKKV IAWISEHGRE LYEKGEMDVE IEGKTFHITR
960 970 980 990 1000
EHVTIEEKLP DFFVAEEFDG GRVFVDKTLT RELLAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IVVTIETTDE NRELLQENLD YIMRETRAVE VRFEEAKGYV
1060
VEWPEVQAKI GIEKV
Length:1,065
Mass (Da):125,512
Last modified:February 15, 2005 - v1
Checksum:i2BC6601CFF79D3A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85937.1.
RefSeqiYP_184161.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85937; BAD85937; TK1748.
GeneIDi3235480.
KEGGitko:TK1748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85937.1 .
RefSeqi YP_184161.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q5JJ31.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 69014.TK1748.

Proteomic databases

PRIDEi Q5JJ31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85937 ; BAD85937 ; TK1748 .
GeneIDi 3235480.
KEGGi tko:TK1748.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
InParanoidi Q5JJ31.
KOi K01870.
OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1784-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiSYI_THEKO
AccessioniPrimary (citable) accession number: Q5JJ31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3