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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei628ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-1755-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TK1748
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985911 – 1065Isoleucine--tRNA ligaseAdd BLAST1065

Proteomic databases

PRIDEiQ5JJ31

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1748

Structurei

3D structure databases

ProteinModelPortaliQ5JJ31
SMRiQ5JJ31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi625 – 629"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
InParanoidiQ5JJ31
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JJ31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIREPEFREY NPGQLEEKIE AFWKENNTYE KVKKLRENGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRSMQGYNV RRQPGFDMHG LPIEVKVEQA
110 120 130 140 150
LKLKNKREIE TKIGVDNFIK KCKEFALNNL KIMTEQFKQL GVWMDWDNPY
160 170 180 190 200
MTIKNEYIES GWFTLKRAWE KGLLEKDKRV LHWCPRCQTA LAEHEVRGEY
210 220 230 240 250
KMRKDPSIYV KFPIEWKEKE YLLIWTTTPW TLPANLAVAA HPEYEYAKVK
260 270 280 290 300
VETENGEEYW IMAKALVERV LSEVGVKGEI VETFKGEELE GIRYTHVLLE
310 320 330 340 350
EYPAQKEFRE KYEWAHRVIL GEHVTLEDGT GLVHTAPGHG EEDFEVGQRY
360 370 380 390 400
GLPVYSPVDD AGRYTEGKWK GVYVKDADPE IIEYLKEKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKVSKVKDQI IKENDEKVTW YPEWVKVRYD
460 470 480 490 500
NGVMNSGDWV ISRQRYWGIP LPIWESEDGE YYVVGSFEEL VKLAVAIEVN
510 520 530 540 550
GERIDLPEGY EEKLKIIEEK LGPEDLHRPY VDAFIIKVNG KEMKRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLDYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
VIAFDTVPYR AVAMHGYVLD EKGDKMSKSL GNIIRPEEVV QKEGRDPFRF
660 670 680 690 700
YMLWATNPWE NLRFSWKGLE QVKRMLNILW NVYVLSATYM SLDNFNPTKV
710 720 730 740 750
NPEELPFREE DRWILSRVNS LIGEVTDGIE TFRLTRATRG IYNFVVEDLS
760 770 780 790 800
RWYIRLIRKR MWVEGDDPDK LAAYYTVWKV FDVLLRLMAP FTPYIAEEIY
810 820 830 840 850
QNLIRPFVGV ESVHMLDWPE ADEKAIDEEL EKEMEYARKI VEAGSSARQK
860 870 880 890 900
ARIKLRYPVR RIIIETEDET VKKAVERINR ILRDQLNAKE VVVGKVEREL
910 920 930 940 950
IIKPNFAKVG PEFKGDAKKV IAWISEHGRE LYEKGEMDVE IEGKTFHITR
960 970 980 990 1000
EHVTIEEKLP DFFVAEEFDG GRVFVDKTLT RELLAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IVVTIETTDE NRELLQENLD YIMRETRAVE VRFEEAKGYV
1060
VEWPEVQAKI GIEKV
Length:1,065
Mass (Da):125,512
Last modified:February 15, 2005 - v1
Checksum:i2BC6601CFF79D3A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD85937.1
RefSeqiWP_011250699.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD85937; BAD85937; TK1748
GeneIDi3235480
KEGGitko:TK1748
PATRICifig|69014.16.peg.1704

Similar proteinsi

Entry informationi

Entry nameiSYI_THEKO
AccessioniPrimary (citable) accession number: Q5JJ31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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