Q5JIQ0 (RTCA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	TK1615

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156434
Regions
	Nucleotide binding	284 – 288	5	ATP By similarity
Sites
	Active site	308	1	Tele-AMP-histidine intermediate By similarity
	Binding site	102	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JIQ0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 07BFF3A9F23BA400	FASTA	343	37,149
	10 20 30 40 50 60 MEWVEIDGSY GEGGGQILRT SVALSVITGK PVRIYNIRAN RPNPGLRPQH LHGILALKEL 70 80 90 100 110 120 SNAKIKGASV GSTELEFIPG KAEPKHVRVP IKTAGSITLV LQALLPAMAF IGGSFEITGG 130 140 150 160 170 180 TDVPWSPPVD YLKHVTLYAL EKMGIKVELE IKRRGHYPRG GGLVVGRIEP WEEKKPLKAL 190 200 210 220 230 240 KWERIEWFAG ISHATNLPAH VAERQAKAAR ERLSEVYSAP VEIETEVSRS LGPGSGIVVW 250 260 270 280 290 300 AETDKLRLGG DALGKRGKPA EVVGREAADE LIEALKTGMA ADRFLGDQLI PFLAFAGGEV 310 320 330 340 GVSEITNHLV TNVWVVEKFF GNVFEVEGEV GKPGTLRVVK SVL

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85804.1.
RefSeq	YP_184028.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JIQ0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000008031; EBPYRP00000007812; EBPYRG00000008030.
GeneID	3235662.
GenomeReviews	Gene locus TK1615 in contig AP006878_GR.
KEGG	tko:TK1615.
NMPDR	fig|69014.3.peg.1946.
Phylogenomic databases
eggNOG	arNOG04553.
GeneTree	EBGT00050000022634.
HOGENOM	HBG683539.
OMA	GGTDVAW.
PhylomeDB	Q5JIQ0.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1615-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PYRKO

Accession

Primary (citable) accession number: Q5JIQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program