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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPBy similarity1
Active sitei308Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi284 – 288ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-1619-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:TK1615
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564341 – 343RNA 3'-terminal phosphate cyclaseAdd BLAST343

Interactioni

Protein-protein interaction databases

STRINGi69014.TK1615

Structurei

3D structure databases

ProteinModelPortaliQ5JIQ0
SMRiQ5JIQ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
HOGENOMiHOG000015264
InParanoidiQ5JIQ0
KOiK18105
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JIQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEWVEIDGSY GEGGGQILRT SVALSVITGK PVRIYNIRAN RPNPGLRPQH
60 70 80 90 100
LHGILALKEL SNAKIKGASV GSTELEFIPG KAEPKHVRVP IKTAGSITLV
110 120 130 140 150
LQALLPAMAF IGGSFEITGG TDVPWSPPVD YLKHVTLYAL EKMGIKVELE
160 170 180 190 200
IKRRGHYPRG GGLVVGRIEP WEEKKPLKAL KWERIEWFAG ISHATNLPAH
210 220 230 240 250
VAERQAKAAR ERLSEVYSAP VEIETEVSRS LGPGSGIVVW AETDKLRLGG
260 270 280 290 300
DALGKRGKPA EVVGREAADE LIEALKTGMA ADRFLGDQLI PFLAFAGGEV
310 320 330 340
GVSEITNHLV TNVWVVEKFF GNVFEVEGEV GKPGTLRVVK SVL
Length:343
Mass (Da):37,149
Last modified:February 15, 2005 - v1
Checksum:i07BFF3A9F23BA400
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD85804.1
RefSeqiWP_011250566.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD85804; BAD85804; TK1615
GeneIDi3235662
KEGGitko:TK1615
PATRICifig|69014.16.peg.1574

Similar proteinsi

Entry informationi

Entry nameiRTCA_THEKO
AccessioniPrimary (citable) accession number: Q5JIQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health