Reviewed, UniProtKB/Swiss-Prot Q5JIQ0 (RTCA_PYRKO)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	TK1615

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

311400 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156434
Sites
	Active site	308	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JIQ0-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 07BFF3A9F23BA400	FASTA	343	37,149
	10 20 30 40 50 60 MEWVEIDGSY GEGGGQILRT SVALSVITGK PVRIYNIRAN RPNPGLRPQH LHGILALKEL 70 80 90 100 110 120 SNAKIKGASV GSTELEFIPG KAEPKHVRVP IKTAGSITLV LQALLPAMAF IGGSFEITGG 130 140 150 160 170 180 TDVPWSPPVD YLKHVTLYAL EKMGIKVELE IKRRGHYPRG GGLVVGRIEP WEEKKPLKAL 190 200 210 220 230 240 KWERIEWFAG ISHATNLPAH VAERQAKAAR ERLSEVYSAP VEIETEVSRS LGPGSGIVVW 250 260 270 280 290 300 AETDKLRLGG DALGKRGKPA EVVGREAADE LIEALKTGMA ADRFLGDQLI PFLAFAGGEV 310 320 330 340 GVSEITNHLV TNVWVVEKFF GNVFEVEGEV GKPGTLRVVK SVL

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85804.1.
RefSeq	YP_184028.1.
3D structure databases
SMR	Q5JIQ0. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5JIQ0.
Genome annotation databases
GeneID	3235662.
GenomeReviews	Gene locus TK1615 in contig AP006878_GR.
KEGG	tko:TK1615.
NMPDR	fig|69014.3.peg.1946.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04553.
HOGENOM	HBG683539.
OMA	EIDGSYG.
PhylomeDB	Q5JIQ0.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1615-MONOMER.
BRENDA	6.5.1.4. 192130.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PYRKO

Accession

Primary (citable) accession number: Q5JIQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)