Q5JIL7 (SYA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	TK1567

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 917	917	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075275
Sites
	Metal binding	615	1	Zinc By similarity
	Metal binding	619	1	Zinc By similarity
	Metal binding	719	1	Zinc By similarity
	Metal binding	723	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JIL7 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: DC7869EBC54F5AA1	FASTA	917	104,882
	10 20 30 40 50 60 MSMDMTTRMF KEEGWIRKKC PKCGKYFWTL DPDRETCGDP PCDEYQFIGK PGIPKKYTLD 70 80 90 100 110 120 EMREKFLSFF EKHESYPHGR VKRYPVLPRW RDDVLLVGAS IMDFQPWVIS GEADPPANPL 130 140 150 160 170 180 TISQPSIRFT DIDNVGITGR HFTIFEMMAH HAFNYPGKPI YWMDETVELA FEFFTKELKM 190 200 210 220 230 240 KPEDITFKEN PWAGGGNAGP AFEVLYRGLE VATLVFMQYK KAPADADPSQ VVEIKGDYYV 250 260 270 280 290 300 PMETRVVDTG YGLERLVWMS HGTPTAYDAV LGYVIEPLKK MAGVEKIDER ILMENSRLAG 310 320 330 340 350 360 MFDIEDMGDL RYLREQVAKR VGISVEELEK AVRPYELIYA IADHTKALTF MLADGVIPSN 370 380 390 400 410 420 VKAGYLARLL IRKSIRHLRE LGLEIPLAEI VAMHIKELSP TFPEFKEMED VILDIINVEE 430 440 450 460 470 480 KRYAETLRRG SDLVKREIAK LKKKGINELP LEKLILFYES HGLTPEIVAE VAEKEGIKVN 490 500 510 520 530 540 IPDNFYTLVA KEAEKQAEKK EAAEYVVDFE LVKDLPDTRT LYYEDPFMKE FDARVLKVIA 550 560 570 580 590 600 DWVVLDQTAF YPEGGGQPYD TGVLEVNGEK VKVTNVQKVG KVILHKVEKP ELFKEGVTVH 610 620 630 640 650 660 GRLDWDRRIQ HMRHHTGTHV LMGALVRVLG KHVWQAGSQL HTDWARLDIS HYKRITEEEL 670 680 690 700 710 720 REIERLANRV VMENRKVTWE WLPRTEAEMK YGFRLYQGGV VPGRVIRVLK IEDWDVQACG 730 740 750 760 770 780 GTHLPNTGLI GPIKILRTER IQDGVERIIF AAGEAAINWM QETERLLKRT AEIFRVPPEK 790 800 810 820 830 840 VPETAERFFN EWKEARKEVE KLRKELAKLL VYELQEKVEK VGNVEFIGAV VEGTIDDLRE 850 860 870 880 890 900 AANRLRKENR VVVLISREGH FVVAVGDGLD LKAGELAKVI TSVAGGGGGG RKELAQGRIK 910 NPLKAEEAIE EVKKMLG

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85756.1.
RefSeq	YP_183980.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JIL7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006145; EBPYRP00000005926; EBPYRG00000006144.
GeneID	3235147.
GenomeReviews	Gene locus TK1567 in contig AP006878_GR.
KEGG	tko:TK1567.
NMPDR	fig|69014.3.peg.679.
Phylogenomic databases
eggNOG	arNOG04130.
GeneTree	EBGT00050000022658.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q5JIL7.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1567-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_PYRKO

Accession

Primary (citable) accession number: Q5JIL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program