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Q5JHP2

- RFCS_THEKO

UniProt

Q5JHP2 - RFCS_THEKO

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Protein
Replication factor C small subunit
Gene
rfcS, TK2218
Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA Inferred.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: InterPro
  3. four-way junction helicase activity Source: InterPro
  4. sequence-specific DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: InterPro
  2. DNA repair Source: InterPro
  3. DNA replication Source: UniProtKB-KW
  4. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-2264-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
Cleaved into the following chain:
Gene namesi
Name:rfcS
Ordered Locus Names:TK2218
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5959Replication factor C small subunit, 1st part Reviewed prediction
PRO_0000030382Add
BLAST
Chaini60 – 599540Pko RFC intein Reviewed prediction
PRO_0000030383Add
BLAST
Chaini600 – 866267Replication factor C small subunit, 2nd part Reviewed prediction
PRO_0000030384Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL) By similarity.

Protein-protein interaction databases

STRINGi69014.TK2218.

Structurei

3D structure databases

ProteinModelPortaliQ5JHP2.
SMRiQ5JHP2. Positions 2-59, 586-866.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini183 – 313131DOD-type homing endonuclease
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154101.
KOiK04801.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamiPF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JHP2-1 [UniParc]FASTAAdd to Basket

« Hide

MSEEVKEVKI LEKPWVEKYR PQRLEDIVGQ DHIVKRLKHY VKTGSMPHLL    50
FAGPPGVGKC LTGDAKVIAN GRLFELGELV EKVSKGRFGP TPVEGLKVLG 100
IDEDGKLREF EVQYVYKDRA ERLIKVRTRL GRELKVTPYH PLLVNRKNGE 150
IMWVKAEELR PGDRLAVPRF LPAIAEEDPL AEWLGYFIGD GHADSKNKVI 200
TFTNTDPSLR QRFMELTERL FPDAKIRERI HKNRAPDVYV NSRRAWELVS 250
SLGLAGRKAD KVYIPEKGWE GIRSFLRAYF DCDAGVDKNA VVLATASREM 300
AEQVTYALAG FGITSKIREK KVRGKTYYHV TISGSENLER FLSEIGFSHR 350
EKLERTLKLV KKPNPNLDSL NVNYELISYV RDRLKLNFSD DKRSWSHRKA 400
RKISWELMKE IYYRLDELER LKESLSRSIL IDWNEMAERR KEIAEKTGIR 450
ADRLLEYIKG KRKPSLRNYI KIAKALGIDL EPTINAMRVF ARKYSSYAEI 500
GRKLGTWNSS VRIILESNTE KIKELEEIRK IELELIGEIL SDEKLKEGVA 550
YLIFLSQNEL YWDEITEVKE LKGDFVIYDL HVPGYHNFIA GNMPTVVHNT 600
TAALALAREL FGENWRHNFL ELNASDERGI NVIREKVKEF ARTKPIGGAS 650
FKIIFLDEAD ALTQDAQQAL RRTMEMFSNN VRFILSCNYS SKIIEPIQSR 700
CAIFRFRPLR DEDIAKRIRY IAENEGLELT EEGLQAILYV AEGDLRRAIN 750
VLQAAAALDT KITDENVFLV ASRARPEDVR EMMTLALEGN FLKAREKLRD 800
ILLRQGLSGE DVLIQMHKEV FNLPIPEDKK VALADKIGEY NFRLVEGANE 850
MIQLEALLAQ FTIMGK 866
Length:866
Mass (Da):99,691
Last modified:February 15, 2005 - v1
Checksum:i0E9386BB07638F0D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD86407.1.
RefSeqiYP_184631.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD86407; BAD86407; TK2218.
GeneIDi3234370.
KEGGitko:TK2218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD86407.1 .
RefSeqi YP_184631.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q5JHP2.
SMRi Q5JHP2. Positions 2-59, 586-866.
ModBasei Search...

Protein-protein interaction databases

STRINGi 69014.TK2218.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD86407 ; BAD86407 ; TK2218 .
GeneIDi 3234370.
KEGGi tko:TK2218.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000154101.
KOi K04801.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-2264-MONOMER.

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view ]
Pfami PF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Gene cloning and function analysis of replication factor C from Thermococcus kodakaraensis KOD1."
    Kitabayashi M., Nishiya Y., Esaka M., Itakura M., Imanaka T.
    Biosci. Biotechnol. Biochem. 67:2373-2380(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-6, FUNCTION.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiRFCS_THEKO
AccessioniPrimary (citable) accession number: Q5JHP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the potential ATP-binding site.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi