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Q5JHP2

- RFCS_THEKO

UniProt

Q5JHP2 - RFCS_THEKO

Protein

Replication factor C small subunit

Gene

rfcS

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. endonuclease activity Source: InterPro
    3. four-way junction helicase activity Source: InterPro
    4. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: InterPro
    2. DNA repair Source: InterPro
    3. DNA replication Source: UniProtKB-KW
    4. intein-mediated protein splicing Source: InterPro

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-2264-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication factor C small subunit
    Short name:
    RFC small subunit
    Alternative name(s):
    Clamp loader small subunit
    Cleaved into the following chain:
    Gene namesi
    Name:rfcS
    Ordered Locus Names:TK2218
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5959Replication factor C small subunit, 1st partSequence AnalysisPRO_0000030382Add
    BLAST
    Chaini60 – 599540Pko RFC inteinSequence AnalysisPRO_0000030383Add
    BLAST
    Chaini600 – 866267Replication factor C small subunit, 2nd partSequence AnalysisPRO_0000030384Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

    Protein-protein interaction databases

    STRINGi69014.TK2218.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JHP2.
    SMRiQ5JHP2. Positions 2-59, 586-866.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini183 – 313131DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1372.
    HOGENOMiHOG000154101.
    KOiK04801.

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    [Graphical view]
    PfamiPF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5JHP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEEVKEVKI LEKPWVEKYR PQRLEDIVGQ DHIVKRLKHY VKTGSMPHLL    50
    FAGPPGVGKC LTGDAKVIAN GRLFELGELV EKVSKGRFGP TPVEGLKVLG 100
    IDEDGKLREF EVQYVYKDRA ERLIKVRTRL GRELKVTPYH PLLVNRKNGE 150
    IMWVKAEELR PGDRLAVPRF LPAIAEEDPL AEWLGYFIGD GHADSKNKVI 200
    TFTNTDPSLR QRFMELTERL FPDAKIRERI HKNRAPDVYV NSRRAWELVS 250
    SLGLAGRKAD KVYIPEKGWE GIRSFLRAYF DCDAGVDKNA VVLATASREM 300
    AEQVTYALAG FGITSKIREK KVRGKTYYHV TISGSENLER FLSEIGFSHR 350
    EKLERTLKLV KKPNPNLDSL NVNYELISYV RDRLKLNFSD DKRSWSHRKA 400
    RKISWELMKE IYYRLDELER LKESLSRSIL IDWNEMAERR KEIAEKTGIR 450
    ADRLLEYIKG KRKPSLRNYI KIAKALGIDL EPTINAMRVF ARKYSSYAEI 500
    GRKLGTWNSS VRIILESNTE KIKELEEIRK IELELIGEIL SDEKLKEGVA 550
    YLIFLSQNEL YWDEITEVKE LKGDFVIYDL HVPGYHNFIA GNMPTVVHNT 600
    TAALALAREL FGENWRHNFL ELNASDERGI NVIREKVKEF ARTKPIGGAS 650
    FKIIFLDEAD ALTQDAQQAL RRTMEMFSNN VRFILSCNYS SKIIEPIQSR 700
    CAIFRFRPLR DEDIAKRIRY IAENEGLELT EEGLQAILYV AEGDLRRAIN 750
    VLQAAAALDT KITDENVFLV ASRARPEDVR EMMTLALEGN FLKAREKLRD 800
    ILLRQGLSGE DVLIQMHKEV FNLPIPEDKK VALADKIGEY NFRLVEGANE 850
    MIQLEALLAQ FTIMGK 866
    Length:866
    Mass (Da):99,691
    Last modified:February 15, 2005 - v1
    Checksum:i0E9386BB07638F0D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD86407.1.
    RefSeqiYP_184631.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD86407; BAD86407; TK2218.
    GeneIDi3234370.
    KEGGitko:TK2218.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD86407.1 .
    RefSeqi YP_184631.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q5JHP2.
    SMRi Q5JHP2. Positions 2-59, 586-866.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 69014.TK2218.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD86407 ; BAD86407 ; TK2218 .
    GeneIDi 3234370.
    KEGGi tko:TK2218.

    Phylogenomic databases

    eggNOGi COG1372.
    HOGENOMi HOG000154101.
    KOi K04801.

    Enzyme and pathway databases

    BioCyci TKOD69014:GH72-2264-MONOMER.

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001387. Cro/C1-type_HTH.
    IPR008824. DNA_helicase_Holl-junc_RuvB_N.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR010982. Lambda_DNA-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR013748. Rep_factorC_C_dom.
    [Graphical view ]
    Pfami PF01381. HTH_3. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    PF08542. Rep_fac_C. 1 hit.
    PF05496. RuvB_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF48019. SSF48019. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.
    2. "Gene cloning and function analysis of replication factor C from Thermococcus kodakaraensis KOD1."
      Kitabayashi M., Nishiya Y., Esaka M., Itakura M., Imanaka T.
      Biosci. Biotechnol. Biochem. 67:2373-2380(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-6, FUNCTION.
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiRFCS_THEKO
    AccessioniPrimary (citable) accession number: Q5JHP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the potential ATP-binding site.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3