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Q5JHP2 (RFCS_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Replication factor C small subunit

Short name=RFC small subunit
Alternative name(s):
Clamp loader small subunit

Cleaved into the following chain:

  1. Pko RFC intein
Gene names
Name:rfcS
Ordered Locus Names:TK2218
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length866 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA Probable. Ref.2

Subunit structure

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL) By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Miscellaneous

The intein interrupts the potential ATP-binding site.

Sequence similarities

Belongs to the activator 1 small subunits family. RfcS subfamily.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5959Replication factor C small subunit, 1st part Potential
PRO_0000030382
Chain60 – 599540Pko RFC intein Potential
PRO_0000030383
Chain600 – 866267Replication factor C small subunit, 2nd part Potential
PRO_0000030384

Regions

Domain183 – 313131DOD-type homing endonuclease

Sequences

Sequence LengthMass (Da)Tools
Q5JHP2 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 0E9386BB07638F0D

FASTA86699,691
        10         20         30         40         50         60 
MSEEVKEVKI LEKPWVEKYR PQRLEDIVGQ DHIVKRLKHY VKTGSMPHLL FAGPPGVGKC 

        70         80         90        100        110        120 
LTGDAKVIAN GRLFELGELV EKVSKGRFGP TPVEGLKVLG IDEDGKLREF EVQYVYKDRA 

       130        140        150        160        170        180 
ERLIKVRTRL GRELKVTPYH PLLVNRKNGE IMWVKAEELR PGDRLAVPRF LPAIAEEDPL 

       190        200        210        220        230        240 
AEWLGYFIGD GHADSKNKVI TFTNTDPSLR QRFMELTERL FPDAKIRERI HKNRAPDVYV 

       250        260        270        280        290        300 
NSRRAWELVS SLGLAGRKAD KVYIPEKGWE GIRSFLRAYF DCDAGVDKNA VVLATASREM 

       310        320        330        340        350        360 
AEQVTYALAG FGITSKIREK KVRGKTYYHV TISGSENLER FLSEIGFSHR EKLERTLKLV 

       370        380        390        400        410        420 
KKPNPNLDSL NVNYELISYV RDRLKLNFSD DKRSWSHRKA RKISWELMKE IYYRLDELER 

       430        440        450        460        470        480 
LKESLSRSIL IDWNEMAERR KEIAEKTGIR ADRLLEYIKG KRKPSLRNYI KIAKALGIDL 

       490        500        510        520        530        540 
EPTINAMRVF ARKYSSYAEI GRKLGTWNSS VRIILESNTE KIKELEEIRK IELELIGEIL 

       550        560        570        580        590        600 
SDEKLKEGVA YLIFLSQNEL YWDEITEVKE LKGDFVIYDL HVPGYHNFIA GNMPTVVHNT 

       610        620        630        640        650        660 
TAALALAREL FGENWRHNFL ELNASDERGI NVIREKVKEF ARTKPIGGAS FKIIFLDEAD 

       670        680        690        700        710        720 
ALTQDAQQAL RRTMEMFSNN VRFILSCNYS SKIIEPIQSR CAIFRFRPLR DEDIAKRIRY 

       730        740        750        760        770        780 
IAENEGLELT EEGLQAILYV AEGDLRRAIN VLQAAAALDT KITDENVFLV ASRARPEDVR 

       790        800        810        820        830        840 
EMMTLALEGN FLKAREKLRD ILLRQGLSGE DVLIQMHKEV FNLPIPEDKK VALADKIGEY 

       850        860 
NFRLVEGANE MIQLEALLAQ FTIMGK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]"Gene cloning and function analysis of replication factor C from Thermococcus kodakaraensis KOD1."
Kitabayashi M., Nishiya Y., Esaka M., Itakura M., Imanaka T.
Biosci. Biotechnol. Biochem. 67:2373-2380(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-6, FUNCTION.
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD86407.1.
RefSeqYP_184631.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JHP2.
SMRQ5JHP2. Positions 2-59, 586-866.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK2218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD86407; BAD86407; TK2218.
GeneID3234370.
KEGGtko:TK2218.

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000154101.
KOK04801.

Enzyme and pathway databases

BioCycTKOD69014:GH72-2264-MONOMER.

Family and domain databases

Gene3D1.10.260.40. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR001387. Cro/C1-type_HTH.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR010982. Lambda_DNA-bd_dom.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamPF01381. HTH_3. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
PF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFCS_THEKO
AccessionPrimary (citable) accession number: Q5JHP2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries