Reviewed, UniProtKB/Swiss-Prot Q5JHB5 (G3P_PYRKO)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	TK0765

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000145732
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	192 – 193	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	167	1	NAD By similarity
	Binding site	298	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JHB5-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 2FB063D9692F7E90	FASTA	334	37,236
	10 20 30 40 50 60 MKVKVGINGY GTIGKRVAYA VSKQDDMELI GVTKTKPDFE AYLARERGIP VYAASEEFLP 70 80 90 100 110 120 RFEKAGFEVA GTLSDLLENV DVIVDATPGG MGAKNKALYE KAGVKAIFQG GEKAEVAQVS 130 140 150 160 170 180 FVAQANYEKA LGKDYVRVVS CNTTGLTRTL SALQEYIDYV YAVMIRRAAD PNDSKRGPVN 190 200 210 220 230 240 AITPSVTVPS HHGPDVQTVI PINIETMAFV VPTTLMHVHS VMIELKKPIT REDVIDIFEN 250 260 270 280 290 300 TTRVLLFEKE RGFESTAQLI EFARDLHREW NNLYEIAVWK ESISVKGNRL FYIQAVHQES 310 320 330 DVVPENVDAI RAMFEMADKW ESIRKTNKSL GILK

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006878 Genomic DNA. Translation: BAD84954.1.
RefSeq	YP_183178.1.
3D structure databases
SMR	Q5JHB5. Positions 1-334.
ModBase	Search...
Genome annotation databases
GeneID	3234767.
GenomeReviews	Gene locus TK0765 in contig AP006878_GR.
KEGG	tko:TK0765.
NMPDR	fig|69014.3.peg.1068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5JHB5.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0765-MON.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006436. Glyceraldehyde-3-P_DHase_2_arc. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_PYRKO

Accession

Primary (citable) accession number: Q5JHB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)