Q5JH91 (G1PDH_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	TK0789

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000157351
Regions
	Nucleotide binding	98 – 102	5	NAD By similarity
	Nucleotide binding	120 – 123	4	NAD By similarity
Sites
	Metal binding	172	1	Zinc; catalytic By similarity
	Metal binding	252	1	Zinc; catalytic By similarity
	Metal binding	268	1	Zinc; catalytic By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	NAD By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JH91 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 67A8EC4FEA4F15F0	FASTA	351	37,756
	10 20 30 40 50 60 MERRIHLMQL PREVLLGENL TGEVVSVAKR IGLTGKALVI YGPKTKEIAG RDVEDAIKSA 70 80 90 100 110 120 YEVSSLTIRK GATMEEVERT IEKIKDEGIG WVIAVGGGSI IDVAKLSSFK TGVPFISFPT 130 140 150 160 170 180 TASHDGIASA NASIKDLGSK TSVKAVPPVA VIADVKVIKT APYRYLAAGV GDTISNLTAV 190 200 210 220 230 240 RDWQLAHRIK GEYYSEYAAS LSLMSAKMVM RNADIIRLGN EESVRKVIKA LISTGVAMSI 250 260 270 280 290 300 AGSSRPASGA EHLFSHALDM LLDKPALHGE QTGLGTIIMA YLHGMKWERV RETLKRVGAP 310 320 330 340 350 TNAYELGIDP EVIIEALTIA HTIRPERYTI LGKDGLTREA AEKAAKITGV I

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84978.1.
RefSeq	YP_183202.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JH91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006418; EBPYRP00000006199; EBPYRG00000006417.
GeneID	3235756.
GenomeReviews	Gene locus TK0789 in contig AP006878_GR.
KEGG	tko:TK0789.
NMPDR	fig|69014.3.peg.1149.
Phylogenomic databases
eggNOG	arNOG04488.
GeneTree	EBGT00050000023171.
HOGENOM	HBG672951.
OMA	DKPALHG.
PhylomeDB	Q5JH91.
ProtClustDB	PRK00843.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0789-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_PYRKO

Accession

Primary (citable) accession number: Q5JH91

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program