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Q5JH16 (SYE_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:TK1408
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 573573Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119729

Regions

Motif107 – 11711"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
Q5JH16 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: C7E5A3C000C2D2D2

FASTA57367,143
        10         20         30         40         50         60 
MEEIRELVLK YALINAYTHR GKANPKAVIG KVLGENPELR KRAKEIIPLV NEVVNEVNSL 

        70         80         90        100        110        120 
SLEEQEAKLR EIYPEFFEEK NEKKEEKKGL PPLPKAERGK VVTRFAPNPD GAFHLGNARA 

       130        140        150        160        170        180 
AILSHEYARM YDGKFILRFD DTDPKVKRPE PIFYEWIKED LEWLGFRIDE IHIASDRLEI 

       190        200        210        220        230        240 
YYDYAEKLIK MGKAYVCTCP PEKFRELRDK GIACPHREEP VEVQLERWRK MLNGEYREGE 

       250        260        270        280        290        300 
AVVRIKTDLN HPNPAVRDWP ALRIIDNPNH PRTGNKYRVW PLYNFASAID DHELGVTHIF 

       310        320        330        340        350        360 
RGQEHAENET RQRYVYEYFG WEYPVTVHHG RLSIEGVILS KSKTRKGIEE GKYLGWDDPR 

       370        380        390        400        410        420 
LGTIRALRRR GIKPEAIREL IIEVGLKRSD TTISWDNLAA INRKIIEPIA NRYFFVADPI 

       430        440        450        460        470        480 
PMYVEGAQEF TAEIPLHPDY PERGVRRLKF EPDKPVYVSK DDMELFKPGN FVRLKDLFNV 

       490        500        510        520        530        540 
EIMEVNENGI RARFHSFEYE VARENRWRMV HWVTEGKACE VWVPEGDELI IREGLLESDA 

       550        560        570 
DVKVDDIVQF ERFGFVRIDD VRPEKVVAIF AHK 

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD85597.1.
RefSeqYP_183821.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JH16.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK1408.

Proteomic databases

PRIDEQ5JH16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85597; BAD85597; TK1408.
GeneID3234725.
KEGGtko:TK1408.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAMRFAPNP.
ProtClustDBPRK04156.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1429-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_THEKO
AccessionPrimary (citable) accession number: Q5JH16
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: February 15, 2005
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries