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Q5JGV6

- HELS_THEKO

UniProt

Q5JGV6 - HELS_THEKO

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Protein
ATP-dependent DNA helicase Hel308
Gene
hel308, TK1332
Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks By similarity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. endonuclease activity Source: UniProtKB-KW
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1352-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent Holliday junction unwindase Hjm
Cleaved into the following chain:
Alternative name(s):
Pko Hel intein
Gene namesi
Name:hel308
Ordered Locus Names:TK1332
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Pathology & Biotechi

Disruption phenotypei

Not essential, it can be disrupted. Slightly more sensitive to UV and mitomycin C than wild-type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331ATP-dependent DNA helicase Hel308, 1st part Reviewed prediction
PRO_0000013302Add
BLAST
Chaini332 – 734403Endonuclease PI-PkoHel Reviewed prediction
PRO_0000013303Add
BLAST
Chaini735 – 1125391ATP-dependent DNA helicase Hel308, 2nd part Reviewed prediction
PRO_0000013304Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1332.

Structurei

3D structure databases

ProteinModelPortaliQ5JGV6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 197165Helicase ATP-binding
Add
BLAST
Domaini226 – 440215Helicase C-terminal
Add
BLAST
Domaini500 – 640141DOD-type homing endonuclease
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 2929Q motifUniRule annotation
Add
BLAST
Motifi145 – 1484DEAH boxUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
KOiK03726.
OMAiRENIGHG.

Family and domain databases

Gene3Di2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_00442. Helicase_Hel308.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JGV6-1 [UniParc]FASTAAdd to Basket

« Hide

MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS     50
GKTLVSEIVM VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA 100
TTGDYDSTDE WLGRYDIIVA TAEKFDSLLR HGASWIKDVK LVVADEVHLI 150
GSYDRGATLE MILTHMLGKA QILALSATVG NAEELAEWLD ASLVVSDWRP 200
VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ ALVFVNTRRS 250
AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF 300
HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE 350
LTEGDEVLTH TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV 400
VKQIRHKSHY SDGRQVIWWE FEGPEWMTAQ ELKERLESET DPKVSYMLLQ 450
PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL HPSVKRTPEY LPLNFETARL 500
IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP HAKIVVKDHE 550
RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL 600
IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT 650
EGIGKTRKPI YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL 700
LKVRSIEEEE YEGDVYNLEV EGDESYSVGF IVHNSAGINL PAFRVIIRDT 750
KRYANFGWTD IPVLEIQQMM GRAGRPKYDK VGEAIIVART EDPKKLIDRY 800
IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF LSRTFYAHQR 850
GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA 900
KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME 950
EHLYSSIPYY EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD 1000
LYRILELADW LMYSLIELYK LFEPKEDVLQ YLRDLHLRLR HGVREELLEL 1050
VKLPNIGRRR ARALYNAGFR SVEDILRAKP KDLLQVEGIG LKIIEGIYRH 1100
LGVEYTISEK EKPKKRKGNL YDFLK 1125
Length:1,125
Mass (Da):129,202
Last modified:February 15, 2005 - v1
Checksum:i1208EEC7AD1045FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1.
RefSeqiYP_183745.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85521; BAD85521; TK1332.
GeneIDi3235744.
KEGGitko:TK1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1 .
RefSeqi YP_183745.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q5JGV6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 69014.TK1332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85521 ; BAD85521 ; TK1332 .
GeneIDi 3235744.
KEGGi tko:TK1332.

Phylogenomic databases

eggNOGi COG1372.
KOi K03726.
OMAi RENIGHG.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1352-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPi MF_00442. Helicase_Hel308.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis."
    Fujikane R., Ishino S., Ishino Y., Forterre P.
    Genes Genet. Syst. 85:243-257(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiHELS_THEKO
AccessioniPrimary (citable) accession number: Q5JGV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi