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Q5JGV6 (HELS_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Putative ski2-type helicase

EC=3.6.4.-

Cleaved into the following chain:

  1. Endonuclease PI-PkoHel
    EC=3.1.-.-
    Alternative name(s):
    Pko Hel intein
Gene names
Ordered Locus Names:TK1332
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length1125 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00442

Sequence similarities

Belongs to the helicase family. SKI2 subfamily.

Contains 1 DOD-type homing endonuclease domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Putative ski2-type helicase, 1st part Potential
PRO_0000013302
Chain332 – 734403Endonuclease PI-PkoHel Potential
PRO_0000013303
Chain735 – 1125391Putative ski2-type helicase, 2nd part Potential
PRO_0000013304

Regions

Domain33 – 197165Helicase ATP-binding
Domain226 – 440215Helicase C-terminal
Domain500 – 640141DOD-type homing endonuclease
Nucleotide binding46 – 538ATP By similarity
Motif1 – 2929Q motif HAMAP-Rule MF_00442
Motif145 – 1484DEAH box HAMAP-Rule MF_00442

Sequences

Sequence LengthMass (Da)Tools
Q5JGV6 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 1208EEC7AD1045FE

FASTA1,125129,202
        10         20         30         40         50         60 
MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS GKTLVSEIVM 

        70         80         90        100        110        120 
VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA TTGDYDSTDE WLGRYDIIVA 

       130        140        150        160        170        180 
TAEKFDSLLR HGASWIKDVK LVVADEVHLI GSYDRGATLE MILTHMLGKA QILALSATVG 

       190        200        210        220        230        240 
NAEELAEWLD ASLVVSDWRP VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ 

       250        260        270        280        290        300 
ALVFVNTRRS AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF 

       310        320        330        340        350        360 
HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE LTEGDEVLTH 

       370        380        390        400        410        420 
TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV VKQIRHKSHY SDGRQVIWWE 

       430        440        450        460        470        480 
FEGPEWMTAQ ELKERLESET DPKVSYMLLQ PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL 

       490        500        510        520        530        540 
HPSVKRTPEY LPLNFETARL IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP 

       550        560        570        580        590        600 
HAKIVVKDHE RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL 

       610        620        630        640        650        660 
IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT EGIGKTRKPI 

       670        680        690        700        710        720 
YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL LKVRSIEEEE YEGDVYNLEV 

       730        740        750        760        770        780 
EGDESYSVGF IVHNSAGINL PAFRVIIRDT KRYANFGWTD IPVLEIQQMM GRAGRPKYDK 

       790        800        810        820        830        840 
VGEAIIVART EDPKKLIDRY IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF 

       850        860        870        880        890        900 
LSRTFYAHQR GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA 

       910        920        930        940        950        960 
KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME EHLYSSIPYY 

       970        980        990       1000       1010       1020 
EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD LYRILELADW LMYSLIELYK 

      1030       1040       1050       1060       1070       1080 
LFEPKEDVLQ YLRDLHLRLR HGVREELLEL VKLPNIGRRR ARALYNAGFR SVEDILRAKP 

      1090       1100       1110       1120 
KDLLQVEGIG LKIIEGIYRH LGVEYTISEK EKPKKRKGNL YDFLK 

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References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD85521.1.
RefSeqYP_183745.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JGV6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK1332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD85521; BAD85521; TK1332.
GeneID3235744.
KEGGtko:TK1332.

Phylogenomic databases

eggNOGCOG1372.
KOK03726.
OMALRENIGH.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1352-MONOMER.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPMF_00442. Helicase_ski2.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHELS_THEKO
AccessionPrimary (citable) accession number: Q5JGV6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries