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Q5JGV6

- HELS_THEKO

UniProt

Q5JGV6 - HELS_THEKO

Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei28 – 281ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 538ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. endonuclease activity Source: UniProtKB-KW
    4. helicase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA repair Source: UniProtKB-HAMAP
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair, Intron homing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1352-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
    Alternative name(s):
    ATP-dependent Holliday junction unwindase Hjm
    Cleaved into the following chain:
    Alternative name(s):
    Pko Hel intein
    Gene namesi
    Name:hel308UniRule annotation
    Ordered Locus Names:TK1332
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Not essential, it can be disrupted. Slightly more sensitive to UV and mitomycin C than wild-type cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 331331ATP-dependent DNA helicase Hel308, 1st partSequence AnalysisPRO_0000013302Add
    BLAST
    Chaini332 – 734403Endonuclease PI-PkoHelSequence AnalysisPRO_0000013303Add
    BLAST
    Chaini735 – 1125391ATP-dependent DNA helicase Hel308, 2nd partSequence AnalysisPRO_0000013304Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi69014.TK1332.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JGV6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 197165Helicase ATP-bindingUniRule annotationAdd
    BLAST
    Domaini226 – 440215Helicase C-terminalUniRule annotationAdd
    BLAST
    Domaini500 – 640141DOD-type homing endonucleaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 2929Q motifAdd
    BLAST
    Motifi145 – 1484DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Hel308 subfamily.UniRule annotation
    Contains 1 helicase ATP-binding domain.UniRule annotation
    Contains 1 helicase C-terminal domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1372.
    KOiK03726.
    OMAiRENIGHG.

    Family and domain databases

    Gene3Di2.170.16.10. 3 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 4 hits.
    HAMAPiMF_00442. Helicase_Hel308.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR028992. Hedgehog/Intein_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR022965. Helicase_ski2.
    IPR003586. Hint_dom_C.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00487. DEXDc. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5JGV6-1 [UniParc]FASTAAdd to Basket

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    MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS     50
    GKTLVSEIVM VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA 100
    TTGDYDSTDE WLGRYDIIVA TAEKFDSLLR HGASWIKDVK LVVADEVHLI 150
    GSYDRGATLE MILTHMLGKA QILALSATVG NAEELAEWLD ASLVVSDWRP 200
    VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ ALVFVNTRRS 250
    AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF 300
    HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE 350
    LTEGDEVLTH TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV 400
    VKQIRHKSHY SDGRQVIWWE FEGPEWMTAQ ELKERLESET DPKVSYMLLQ 450
    PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL HPSVKRTPEY LPLNFETARL 500
    IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP HAKIVVKDHE 550
    RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL 600
    IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT 650
    EGIGKTRKPI YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL 700
    LKVRSIEEEE YEGDVYNLEV EGDESYSVGF IVHNSAGINL PAFRVIIRDT 750
    KRYANFGWTD IPVLEIQQMM GRAGRPKYDK VGEAIIVART EDPKKLIDRY 800
    IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF LSRTFYAHQR 850
    GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA 900
    KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME 950
    EHLYSSIPYY EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD 1000
    LYRILELADW LMYSLIELYK LFEPKEDVLQ YLRDLHLRLR HGVREELLEL 1050
    VKLPNIGRRR ARALYNAGFR SVEDILRAKP KDLLQVEGIG LKIIEGIYRH 1100
    LGVEYTISEK EKPKKRKGNL YDFLK 1125
    Length:1,125
    Mass (Da):129,202
    Last modified:February 15, 2005 - v1
    Checksum:i1208EEC7AD1045FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD85521.1.
    RefSeqiYP_183745.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD85521; BAD85521; TK1332.
    GeneIDi3235744.
    KEGGitko:TK1332.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD85521.1 .
    RefSeqi YP_183745.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q5JGV6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 69014.TK1332.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD85521 ; BAD85521 ; TK1332 .
    GeneIDi 3235744.
    KEGGi tko:TK1332.

    Phylogenomic databases

    eggNOGi COG1372.
    KOi K03726.
    OMAi RENIGHG.

    Enzyme and pathway databases

    BioCyci TKOD69014:GH72-1352-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 3 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 4 hits.
    HAMAPi MF_00442. Helicase_Hel308.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR028992. Hedgehog/Intein_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR022965. Helicase_ski2.
    IPR003586. Hint_dom_C.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00487. DEXDc. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 1 hit.
    SSF52540. SSF52540. 3 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.
    2. "Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis."
      Fujikane R., Ishino S., Ishino Y., Forterre P.
      Genes Genet. Syst. 85:243-257(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiHELS_THEKO
    AccessioniPrimary (citable) accession number: Q5JGV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3