Q5JGV6 (HELS_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ski2-type helicase
EC=3.6.4.-

Cleaved into the following chain:

1. Endonuclease PI-PkoHel
   EC=3.1.-.-
   Alternative name(s):
   Pko Hel intein

Gene names

Ordered Locus Names:

TK1332

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus kodakarensis

Protein attributes

Sequence length	1125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00442
Sequence similarities	Belongs to the helicase family. SKI2 subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Putative ski2-type helicase, 1st part Potential		PRO_0000013302
	Chain	332 – 734	403	Endonuclease PI-PkoHel Potential		PRO_0000013303
	Chain	735 – 1125	391	Putative ski2-type helicase, 2nd part Potential		PRO_0000013304
Regions
	Domain	33 – 197	165	Helicase ATP-binding
	Domain	226 – 440	215	Helicase C-terminal
	Domain	500 – 640	141	DOD-type homing endonuclease
	Nucleotide binding	46 – 53	8	ATP By similarity
	Motif	1 – 29	29	Q motif HAMAP-Rule MF_00442
	Motif	145 – 148	4	DEAH box HAMAP-Rule MF_00442

Sequences

	Sequence		Length	Mass (Da)
	Q5JGV6 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 1208EEC7AD1045FE	FASTA	1,125	129,202
	10 20 30 40 50 60 MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS GKTLVSEIVM 70 80 90 100 110 120 VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA TTGDYDSTDE WLGRYDIIVA 130 140 150 160 170 180 TAEKFDSLLR HGASWIKDVK LVVADEVHLI GSYDRGATLE MILTHMLGKA QILALSATVG 190 200 210 220 230 240 NAEELAEWLD ASLVVSDWRP VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ 250 260 270 280 290 300 ALVFVNTRRS AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF 310 320 330 340 350 360 HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE LTEGDEVLTH 370 380 390 400 410 420 TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV VKQIRHKSHY SDGRQVIWWE 430 440 450 460 470 480 FEGPEWMTAQ ELKERLESET DPKVSYMLLQ PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL 490 500 510 520 530 540 HPSVKRTPEY LPLNFETARL IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP 550 560 570 580 590 600 HAKIVVKDHE RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL 610 620 630 640 650 660 IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT EGIGKTRKPI 670 680 690 700 710 720 YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL LKVRSIEEEE YEGDVYNLEV 730 740 750 760 770 780 EGDESYSVGF IVHNSAGINL PAFRVIIRDT KRYANFGWTD IPVLEIQQMM GRAGRPKYDK 790 800 810 820 830 840 VGEAIIVART EDPKKLIDRY IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF 850 860 870 880 890 900 LSRTFYAHQR GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA 910 920 930 940 950 960 KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME EHLYSSIPYY 970 980 990 1000 1010 1020 EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD LYRILELADW LMYSLIELYK 1030 1040 1050 1060 1070 1080 LFEPKEDVLQ YLRDLHLRLR HGVREELLEL VKLPNIGRRR ARALYNAGFR SVEDILRAKP 1090 1100 1110 1120 KDLLQVEGIG LKIIEGIYRH LGVEYTISEK EKPKKRKGNL YDFLK

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85521.1.
RefSeq	YP_183745.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JGV6.
ModBase	Search...
Protein-protein interaction databases
STRING	69014.TK1332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD85521; BAD85521; TK1332.
GeneID	3235744.
KEGG	tko:TK1332.
Phylogenomic databases
eggNOG	COG1372.
KO	K03726.
Family and domain databases
HAMAP	MF_00442. Helicase_ski2.
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR022965. Helicase_ski2. IPR003586. Hint_dom_C. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR014014. RNA_helicase_DEAD_Q_motif. IPR010994. RuvA_2-like. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00487. DEXDc. 1 hit. SM00278. HhH1. 2 hits. SM00305. HintC. 1 hit. [Graphical view]
SUPFAM	SSF47781. RuvA_2_like. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HELS_PYRKO

Accession

Primary (citable) accession number: Q5JGV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program