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Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, Intron homing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent Holliday junction unwindase Hjm
Cleaved into the following chain:
Alternative name(s):
Pko Hel intein
Gene namesi
Name:hel308UniRule annotation
Ordered Locus Names:TK1332
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Not essential, it can be disrupted. Slightly more sensitive to UV and mitomycin C than wild-type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000133021 – 331ATP-dependent DNA helicase Hel308, 1st partSequence analysisAdd BLAST331
ChainiPRO_0000013303332 – 734Endonuclease PI-PkoHelSequence analysisAdd BLAST403
ChainiPRO_0000013304735 – 1125ATP-dependent DNA helicase Hel308, 2nd partSequence analysisAdd BLAST391

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiQ5JGV6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1332

Structurei

3D structure databases

ProteinModelPortaliQ5JGV6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 197Helicase ATP-bindingUniRule annotationAdd BLAST165
Domaini226 – 440Helicase C-terminalUniRule annotationAdd BLAST215
Domaini500 – 640DOD-type homing endonucleaseAdd BLAST141

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 29Q motifAdd BLAST29
Motifi145 – 148DEAH box4

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00553 Archaea
COG1204 LUCA
COG1372 LUCA
InParanoidiQ5JGV6
KOiK03726
OrthoDBiPOG093Z00MB

Family and domain databases

Gene3Di3.10.28.10, 1 hit
HAMAPiMF_00442 Helicase_Hel308, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR022965 Helicase_Hel308
IPR003586 Hint_dom_C
IPR036844 Hint_dom_sf
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF14528 LAGLIDADG_3, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00278 HhH1, 2 hits
SM00305 HintC, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF51294 SSF51294, 1 hit
SSF52540 SSF52540, 3 hits
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JGV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS
60 70 80 90 100
GKTLVSEIVM VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA
110 120 130 140 150
TTGDYDSTDE WLGRYDIIVA TAEKFDSLLR HGASWIKDVK LVVADEVHLI
160 170 180 190 200
GSYDRGATLE MILTHMLGKA QILALSATVG NAEELAEWLD ASLVVSDWRP
210 220 230 240 250
VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ ALVFVNTRRS
260 270 280 290 300
AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF
310 320 330 340 350
HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE
360 370 380 390 400
LTEGDEVLTH TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV
410 420 430 440 450
VKQIRHKSHY SDGRQVIWWE FEGPEWMTAQ ELKERLESET DPKVSYMLLQ
460 470 480 490 500
PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL HPSVKRTPEY LPLNFETARL
510 520 530 540 550
IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP HAKIVVKDHE
560 570 580 590 600
RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL
610 620 630 640 650
IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT
660 670 680 690 700
EGIGKTRKPI YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL
710 720 730 740 750
LKVRSIEEEE YEGDVYNLEV EGDESYSVGF IVHNSAGINL PAFRVIIRDT
760 770 780 790 800
KRYANFGWTD IPVLEIQQMM GRAGRPKYDK VGEAIIVART EDPKKLIDRY
810 820 830 840 850
IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF LSRTFYAHQR
860 870 880 890 900
GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA
910 920 930 940 950
KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME
960 970 980 990 1000
EHLYSSIPYY EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD
1010 1020 1030 1040 1050
LYRILELADW LMYSLIELYK LFEPKEDVLQ YLRDLHLRLR HGVREELLEL
1060 1070 1080 1090 1100
VKLPNIGRRR ARALYNAGFR SVEDILRAKP KDLLQVEGIG LKIIEGIYRH
1110 1120
LGVEYTISEK EKPKKRKGNL YDFLK
Length:1,125
Mass (Da):129,202
Last modified:February 15, 2005 - v1
Checksum:i1208EEC7AD1045FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD85521.1

Genome annotation databases

EnsemblBacteriaiBAD85521; BAD85521; TK1332
KEGGitko:TK1332
PATRICifig|69014.16.peg.1304

Similar proteinsi

Entry informationi

Entry nameiHELS_THEKO
AccessioniPrimary (citable) accession number: Q5JGV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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