Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent Holliday junction unwindase Hjm
Cleaved into the following chain:
Alternative name(s):
Pko Hel intein
Gene namesi
Name:hel308UniRule annotation
Ordered Locus Names:TK1332
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Not essential, it can be disrupted. Slightly more sensitive to UV and mitomycin C than wild-type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000133021 – 331ATP-dependent DNA helicase Hel308, 1st partSequence analysisAdd BLAST331
ChainiPRO_0000013303332 – 734Endonuclease PI-PkoHelSequence analysisAdd BLAST403
ChainiPRO_0000013304735 – 1125ATP-dependent DNA helicase Hel308, 2nd partSequence analysisAdd BLAST391

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1332.

Structurei

3D structure databases

ProteinModelPortaliQ5JGV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 197Helicase ATP-bindingUniRule annotationAdd BLAST165
Domaini226 – 440Helicase C-terminalUniRule annotationAdd BLAST215
Domaini500 – 640DOD-type homing endonucleaseAdd BLAST141

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 29Q motifAdd BLAST29
Motifi145 – 148DEAH box4

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
COG1204. LUCA.
COG1372. LUCA.
InParanoidiQ5JGV6.
KOiK03726.

Family and domain databases

Gene3Di2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_Hel308.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JGV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS
60 70 80 90 100
GKTLVSEIVM VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA
110 120 130 140 150
TTGDYDSTDE WLGRYDIIVA TAEKFDSLLR HGASWIKDVK LVVADEVHLI
160 170 180 190 200
GSYDRGATLE MILTHMLGKA QILALSATVG NAEELAEWLD ASLVVSDWRP
210 220 230 240 250
VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ ALVFVNTRRS
260 270 280 290 300
AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF
310 320 330 340 350
HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE
360 370 380 390 400
LTEGDEVLTH TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV
410 420 430 440 450
VKQIRHKSHY SDGRQVIWWE FEGPEWMTAQ ELKERLESET DPKVSYMLLQ
460 470 480 490 500
PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL HPSVKRTPEY LPLNFETARL
510 520 530 540 550
IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP HAKIVVKDHE
560 570 580 590 600
RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL
610 620 630 640 650
IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT
660 670 680 690 700
EGIGKTRKPI YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL
710 720 730 740 750
LKVRSIEEEE YEGDVYNLEV EGDESYSVGF IVHNSAGINL PAFRVIIRDT
760 770 780 790 800
KRYANFGWTD IPVLEIQQMM GRAGRPKYDK VGEAIIVART EDPKKLIDRY
810 820 830 840 850
IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF LSRTFYAHQR
860 870 880 890 900
GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA
910 920 930 940 950
KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME
960 970 980 990 1000
EHLYSSIPYY EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD
1010 1020 1030 1040 1050
LYRILELADW LMYSLIELYK LFEPKEDVLQ YLRDLHLRLR HGVREELLEL
1060 1070 1080 1090 1100
VKLPNIGRRR ARALYNAGFR SVEDILRAKP KDLLQVEGIG LKIIEGIYRH
1110 1120
LGVEYTISEK EKPKKRKGNL YDFLK
Length:1,125
Mass (Da):129,202
Last modified:February 15, 2005 - v1
Checksum:i1208EEC7AD1045FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1.

Genome annotation databases

EnsemblBacteriaiBAD85521; BAD85521; TK1332.
KEGGitko:TK1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1.

3D structure databases

ProteinModelPortaliQ5JGV6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK1332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85521; BAD85521; TK1332.
KEGGitko:TK1332.

Phylogenomic databases

eggNOGiarCOG00553. Archaea.
COG1204. LUCA.
COG1372. LUCA.
InParanoidiQ5JGV6.
KOiK03726.

Family and domain databases

Gene3Di2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_00442. Helicase_Hel308. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_Hel308.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHELS_THEKO
AccessioniPrimary (citable) accession number: Q5JGV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.