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Q5JGV6

- HELS_THEKO

UniProt

Q5JGV6 - HELS_THEKO

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Protein

ATP-dependent DNA helicase Hel308

Gene

hel308

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. endonuclease activity Source: UniProtKB-KW
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1352-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase Hel308UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent Holliday junction unwindase Hjm
Cleaved into the following chain:
Alternative name(s):
Pko Hel intein
Gene namesi
Name:hel308UniRule annotation
Ordered Locus Names:TK1332
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Pathology & Biotechi

Disruption phenotypei

Not essential, it can be disrupted. Slightly more sensitive to UV and mitomycin C than wild-type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331ATP-dependent DNA helicase Hel308, 1st partSequence AnalysisPRO_0000013302Add
BLAST
Chaini332 – 734403Endonuclease PI-PkoHelSequence AnalysisPRO_0000013303Add
BLAST
Chaini735 – 1125391ATP-dependent DNA helicase Hel308, 2nd partSequence AnalysisPRO_0000013304Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK1332.

Structurei

3D structure databases

ProteinModelPortaliQ5JGV6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 197165Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini226 – 440215Helicase C-terminalUniRule annotationAdd
BLAST
Domaini500 – 640141DOD-type homing endonucleaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 2929Q motifAdd
BLAST
Motifi145 – 1484DEAH box

Sequence similaritiesi

Belongs to the helicase family. Hel308 subfamily.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1372.
InParanoidiQ5JGV6.
KOiK03726.
OMAiRENIGHG.

Family and domain databases

Gene3Di2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPiMF_00442. Helicase_Hel308.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JGV6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVDELPIDE RIKRVIKERG IEELYPPQAE ALKSGVLEGK NLVLAIPTAS
60 70 80 90 100
GKTLVSEIVM VNKLLSEGGK AVYLVPLKAL AEEKYREFKE WEVLGLRVAA
110 120 130 140 150
TTGDYDSTDE WLGRYDIIVA TAEKFDSLLR HGASWIKDVK LVVADEVHLI
160 170 180 190 200
GSYDRGATLE MILTHMLGKA QILALSATVG NAEELAEWLD ASLVVSDWRP
210 220 230 240 250
VELRKGVFHL GQLFWEDGKI DHYPENWESL VLDAVKKGKQ ALVFVNTRRS
260 270 280 290 300
AEKEAISLSS KVSKLLTKPE TRRLEELISS IEDNPTTEKL KRALKGGVAF
310 320 330 340 350
HHAGLSRAER TLIEDAFRNG LIKVITATPT LCMHPDTYVV TKSGAKKVSE
360 370 380 390 400
LTEGDEVLTH TGTFKKVIQP LRREHKGRLL VIKAYGTVPV KITPEHMVWV
410 420 430 440 450
VKQIRHKSHY SDGRQVIWWE FEGPEWMTAQ ELKERLESET DPKVSYMLLQ
460 470 480 490 500
PIPEPSVDAD KIPLRKEVYV VNQHGKTDKL HPSVKRTPEY LPLNFETARL
510 520 530 540 550
IGLWIAEGST SKNGVIKFDI SSNEEDLTEF ITGTIRKYFP HAKIVVKDHE
560 570 580 590 600
RNRRTVRFCN KRFAEWLREN IGHGADNKSI PPLLLLNKNR EVRLGLLRGL
610 620 630 640 650
IEGDGYVRRE SQRRANYISY STVSPSLAYQ LQLLVASLGY TSSIHRSIRT
660 670 680 690 700
EGIGKTRKPI YDVKVSGKSY YSLLEELGFE VPQRGNRTYN VNRTWKNYLL
710 720 730 740 750
LKVRSIEEEE YEGDVYNLEV EGDESYSVGF IVHNSAGINL PAFRVIIRDT
760 770 780 790 800
KRYANFGWTD IPVLEIQQMM GRAGRPKYDK VGEAIIVART EDPKKLIDRY
810 820 830 840 850
IHGKPEKLFS MLANEQAFRS QVLALITNFG VEDFRELVDF LSRTFYAHQR
860 870 880 890 900
GDTSSLEYKA KDIVYFLIEN EFIDMDVENR FIALPFGRRT SQLYIDPLTA
910 920 930 940 950
KKFKDAFPAI ERNPNPFGIF QLLASTPDMA TLTARRREME DYLDLAYEME
960 970 980 990 1000
EHLYSSIPYY EDSRFQGFLG QVKTAKVLLD WINEVPEARI YETYNIDPGD
1010 1020 1030 1040 1050
LYRILELADW LMYSLIELYK LFEPKEDVLQ YLRDLHLRLR HGVREELLEL
1060 1070 1080 1090 1100
VKLPNIGRRR ARALYNAGFR SVEDILRAKP KDLLQVEGIG LKIIEGIYRH
1110 1120
LGVEYTISEK EKPKKRKGNL YDFLK
Length:1,125
Mass (Da):129,202
Last modified:February 15, 2005 - v1
Checksum:i1208EEC7AD1045FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1.
RefSeqiYP_183745.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85521; BAD85521; TK1332.
GeneIDi3235744.
KEGGitko:TK1332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD85521.1 .
RefSeqi YP_183745.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q5JGV6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 69014.TK1332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85521 ; BAD85521 ; TK1332 .
GeneIDi 3235744.
KEGGi tko:TK1332.

Phylogenomic databases

eggNOGi COG1372.
InParanoidi Q5JGV6.
KOi K03726.
OMAi RENIGHG.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1352-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 3 hits.
3.10.28.10. 1 hit.
3.40.50.300. 4 hits.
HAMAPi MF_00442. Helicase_Hel308.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR028992. Hedgehog/Intein_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR022965. Helicase_ski2.
IPR003586. Hint_dom_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00487. DEXDc. 1 hit.
SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis."
    Fujikane R., Ishino S., Ishino Y., Forterre P.
    Genes Genet. Syst. 85:243-257(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiHELS_THEKO
AccessioniPrimary (citable) accession number: Q5JGV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3