Q5JGN9 (RIFK_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	TK1272

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Riboflavin kinase HAMAP MF_01285		PRO_0000322105
Regions
	Nucleotide binding	95 – 100	6	CDP By similarity
	Nucleotide binding	191 – 194	4	CDP By similarity
	Region	1 – 85	85	H-T-H motif-like HAMAP MF_01285
	Region	86 – 211	126	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	122	1	Magnesium By similarity
	Metal binding	124	1	Magnesium By similarity
	Binding site	178	1	FMN By similarity
	Binding site	186	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JGN9 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 97B9AFA0DD0A78AA	FASTA	211	23,441
	10 20 30 40 50 60 MKKILMLIEL SERNAIGRPV RITIRELADA LNTSPQTVLR LLAELEDEGL IERKTEGRRT 70 80 90 100 110 120 YIEILPKGLD FLQDICDKIS NALSKGVIVG EVVSGLGEGA YYVRQYEPLI EEYLGFKPFP 130 140 150 160 170 180 GTLNVKILFP KTVLDAVCNI RPVIIPGFVK DGRTFGDVKA YPVMIGGIKG AIVVPSRTIH 190 200 210 PPRIAEIIAP VNLREALKLK DGDRVRLEVI Q

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD85461.1.
RefSeq	YP_183685.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JGN9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006177; EBPYRP00000005958; EBPYRG00000006176.
GeneID	3233889.
GenomeReviews	Gene locus TK1272 in contig AP006878_GR.
KEGG	tko:TK1272.
NMPDR	fig|69014.3.peg.848.
Phylogenomic databases
eggNOG	arNOG10832.
GeneTree	EBGT00050000023005.
HOGENOM	HBG553349.
OMA	GRHYISL.
PhylomeDB	Q5JGN9.
ProtClustDB	CLSK253495.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1272-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR012318. HTH_CRP_2. IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR001808. Tscrpt_reg_HTH_Crp. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
SMART	SM00419. HTH_CRP. 1 hit. [Graphical view]
PROSITE	PS51063. HTH_CRP_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRKO

Accession

Primary (citable) accession number: Q5JGN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 15, 2005
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program