Reviewed, UniProtKB/Swiss-Prot Q5JGD1 (AMPM_PYRKO)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	TK1183

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

311400 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Methionine aminopeptidase		PRO_0000148980
Sites
	Metal binding	83	1	Cobalt 1 By similarity
	Metal binding	94	1	Cobalt 1 By similarity
	Metal binding	94	1	Cobalt 2 By similarity
	Metal binding	154	1	Cobalt 2 By similarity
	Metal binding	188	1	Cobalt 2 By similarity
	Metal binding	281	1	Cobalt 1 By similarity
	Metal binding	281	1	Cobalt 2 By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JGD1-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4199B9ADA77AD173	FASTA	295	33,021
	10 20 30 40 50 60 MDEREALIKA GEIARQVKKE VVDLIKPGAK LYDIAEFVER RIVELGGKPA FPCNLSINEI 70 80 90 100 110 120 AAHYTPYKGD GTVLKEGDYL KLDIGVHVDG YIADTAVTFR VGMEEDELME AAREALENAI 130 140 150 160 170 180 ATVRAGVMIR DVARAIEETI RGKGFNPIVN LSGHKVERYK LHAGVSVPNV YREADTYVLQ 190 200 210 220 230 240 EGDVFAIEPF ATTGAGQVIE VPPALIFMYL RDRPVRMLQA RRLLMHIKKN YKTLPFAYRW 250 260 270 280 290 LQDFLPEGQL KLALAQLEKA GAIYAYPILR EVRGGMVAQF EHTVIVEKEG AYITT

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006878 Genomic DNA. Translation: BAD85372.1.
RefSeq	YP_183596.1.
3D structure databases
SMR	Q5JGD1. Positions 3-295.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5JGD1.
Protein family/group databases
MEROPS	M24.002.
Genome annotation databases
GeneID	3234128.
GenomeReviews	Gene locus TK1183 in contig AP006878_GR.
KEGG	tko:TK1183.
NMPDR	fig|69014.3.peg.1534.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5JGD1.
OMA	GHKIERY.
Enzyme and pathway databases
BioCyc	TKOD69014:TK1183-MON.
BRENDA	3.4.11.18. 192130.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00501. met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_PYRKO

Accession

Primary (citable) accession number: Q5JGD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)