Q5JFT3 (AROA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	TK0263

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088336

Sequences

	Sequence		Length	Mass (Da)
	Q5JFT3 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 8FB32B8007A824B6	FASTA	399	43,119
	10 20 30 40 50 60 MIIRPVDEVR GELNAPPSKS YTHRAYFLAL LAEGESTIEN PLVCDDTLAT IEAIRSFGAG 70 80 90 100 110 120 VDGKTVVPPE EPSPGFVYAR ESGTTARFST ALAGGIGGKT LIDGARRLRE RPMDGLVKAL 130 140 150 160 170 180 KGLGAEVDGF SLPLTVKGPV KSGRVSVDAS KSSQFVSGLL LLGAEVGLKV EARNPVSKPY 190 200 210 220 230 240 IEMTLRTMEA FGVEFEREGF SFEVYPGVKG TRYKVPGDYS TASFFLAAGA LYGKVRVNNL 250 260 270 280 290 300 LREDVQADMA FLDALEEFGA RVKRGRDYVK VEGGELKAVA LDCSDFPDSF PILAVVAAYA 310 320 330 340 350 360 EGRSVIRARQ LRFKESDRVR AMAVNLSRMG VKVRELEDGL EIEGGRPRGA KVETFNDHRI 370 380 390 AMAMSIAALG ATGPSIIEDT ESVSKSHPGF FDDLRRLLE

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84452.1.
RefSeq	YP_182676.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JFT3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000007847; EBPYRP00000007628; EBPYRG00000007846.
GeneID	3235874.
GenomeReviews	Gene locus TK0263 in contig AP006878_GR.
KEGG	tko:TK0263.
NMPDR	fig|69014.3.peg.10.
Phylogenomic databases
eggNOG	arNOG05032.
GeneTree	EBGT00050000023163.
HOGENOM	HBG646626.
OMA	ERKSWSF.
PhylomeDB	Q5JFT3.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0263-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_PYRKO

Accession

Primary (citable) accession number: Q5JFT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program