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Q5JFP1 (PUR2_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:TK0204
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151517

Regions

Domain107 – 315209ATP-grasp
Nucleotide binding134 – 19360ATP By similarity

Sites

Metal binding2731Magnesium or manganese 1 By similarity
Metal binding2851Magnesium or manganese 1 By similarity
Metal binding2851Magnesium or manganese 2 By similarity
Metal binding2871Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5JFP1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: CFFF66298DF5BC37

FASTA43147,925
        10         20         30         40         50         60 
MRVLLVGAGG RENAIAEVLA RDAELYVVAG HKNPGIARLA KDYALAKETD VPRVLDFALK 

        70         80         90        100        110        120 
WGVDMAFIGP EAPLEKGIVN VLEENGVPTV GPTREAAMLE TNKAFARWLM EEYKIPGRKL 

       130        140        150        160        170        180 
FKVFDDVSEM KSWIDDFGRP VVVKPLGLTG GKGVKVVGYQ LKDNEEAKAY AEELIKRDGK 

       190        200        210        220        230        240 
VLIEERTDGV EFTFQVFTDG KRVVPMPLAQ DYPHAYEGDV GPITGGMGSY SCEDHRLPFI 

       250        260        270        280        290        300 
TKEDYEKALE TLKATVEAMR KNGTPYKGIL YGQFMLAKDE PKIIEFNARF GDPEAMNVLP 

       310        320        330        340        350        360 
ILKRSLVEIG EEIVDGNLKG AEFERKATVV KYIAPKGYPT NPVRGIKLHV DEAKIREEGA 

       370        380        390        400        410        420 
RVYYASLDEN FRMLGSRALA VVGIADSLEE AERIASAGIR HVRGEIFYRK DVGTRESIAR 

       430 
RIELVRAMRG E 

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD84393.1.
RefSeqYP_182617.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JFP1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK0204.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD84393; BAD84393; TK0204.
GeneID3234772.
KEGGtko:TK0204.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycTKOD69014:GH72-206-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_THEKO
AccessionPrimary (citable) accession number: Q5JFP1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways