Q5JFM4 (GUAAA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	TK0190

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140231
Regions
	Domain	1 – 188	188	Glutamine amidotransferase type-1
Sites
	Active site	78	1	Nucleophile By similarity
	Active site	165	1	By similarity
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JFM4 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4074B943E749C029	FASTA	188	21,203
	10 20 30 40 50 60 MIVIMDNGGQ YVHRIWRTLR YLGVEAKIIP NTTPLEEIKA MKPKGIIFSG GPDINKTGNC 70 80 90 100 110 120 SAILEHYDEF NVPILGICLG HQLIAKHFGG KVGRGEKAEY SLVEIEILDE NDIFRGLPRK 130 140 150 160 170 180 LRVWESHMDE VKELPPGFKL LARSETCPVE AMKHESLPIY GVQFHPEVAH TEHGADIYRN FAELCGEL

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84379.1.
RefSeq	YP_182603.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JFM4.
SMR	Q5JFM4. Positions 1-187.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006618; EBPYRP00000006399; EBPYRG00000006617.
GeneID	3235912.
GenomeReviews	Gene locus TK0190 in contig AP006878_GR.
KEGG	tko:TK0190.
NMPDR	fig|69014.3.peg.418.
Phylogenomic databases
eggNOG	arNOG05587.
GeneTree	EBGT00050000022457.
HOGENOM	HBG292341.
OMA	GQYVHRI.
PhylomeDB	Q5JFM4.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0190-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_PYRKO

Accession

Primary (citable) accession number: Q5JFM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program