Q5JFM1 (GUAAB_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	TK0193

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140250
Regions
	Domain	1 – 184	184	GMPS ATP-PPase
	Nucleotide binding	27 – 33	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JFM1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 725077FE88F5EF99	FASTA	307	34,467
	10 20 30 40 50 60 MWENFIEEKV KEIRETVGDG KAIIALSGGV DSSTAAVLAH RAIGDRLHAV FVNTGFMRKG 70 80 90 100 110 120 EPEFVVKTFR DEFGLNLHYV DASERFFREL KGVTDPEEKR KIIGRVFIEV FEEVAKEINA 130 140 150 160 170 180 DFLIQGTIAP DWIESHGKIK SHHNVGGLPE RLNLKLIEPL RDLYKDEVRE LAKELGLPEK 190 200 210 220 230 240 IYNRMPFPGP GLAVRVLGEV TPERVAIVRE ANAIVEEEIE KAGLKPWQAF AVLLGVKTVG 250 260 270 280 290 300 VQGDIRAYKE TVAVRVVESL DGMTANAMNV PWEVLQRIAF RITSEIPEVG RVLYDITNKP PATIEFE

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84382.1.
RefSeq	YP_182606.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JFM1.
SMR	Q5JFM1. Positions 2-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000007419; EBPYRP00000007200; EBPYRG00000007418.
GeneID	3233684.
GenomeReviews	Gene locus TK0193 in contig AP006878_GR.
KEGG	tko:TK0193.
NMPDR	fig|69014.3.peg.421.
Phylogenomic databases
eggNOG	arNOG04539.
GeneTree	EBGT00050000022268.
HOGENOM	HBG391775.
OMA	QGTIAPD.
PhylomeDB	Q5JFM1.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0193-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_PYRKO

Accession

Primary (citable) accession number: Q5JFM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	February 15, 2005
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program