Q5JFL7 (PURL_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	TK0197

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100523
Regions
	Nucleotide binding	89 – 100	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5JFL7 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 2E07AB16A60D6A15	FASTA	713	78,396
	10 20 30 40 50 60 MFPHEEKLIR ERLGREPNEV EKAMLEVMWS EHASYKSSRP FLKLLPTENE HVVLGPGEDA 70 80 90 100 110 120 GIVKFDDETW IAVGIESHNH PSAVEPYGGA ATGVGGIVRD ILCMGARPIA LLDPIRFGPL 130 140 150 160 170 180 EKERNRYLLQ GVVKGIADYG NRIGVPTVGG ETEFDESLDN YTLVNVACVG IMRPEHFVHS 190 200 210 220 230 240 YVEEAGLKLV LVGNRTGRDG IHGVTFASEE LSENAEEEDR SAVQIPDPFT EKLLIEATLE 250 260 270 280 290 300 AVYTGKVRAL KDLGGGGLTC ASSEMAGKKG FGAVIYADRV PLREPNMTPT EVMISESQER 310 320 330 340 350 360 MLFAVREEDV EEIGKIFEKY GLEWTVVGEI IKEPRYIVYW KGEKVADLPI DLLTEVPTIE 370 380 390 400 410 420 WETRPYSLER DVETPKISFS DAFELVWSSP NVLNKRWVWE QYDHEVQGRT VVKPGRDAAV 430 440 450 460 470 480 LKINERYGLA FVADGNPNHS HLNPYHGAMG AVAEVVRNLV SVGAEPLALV DNLNFASPER 490 500 510 520 530 540 PEVYWSFAET VRGLADAARA FGLAYVSGNV SFYNEVVDRP IKPTPVVAGL GKVELEKIPG 550 560 570 580 590 600 FGLEEGLLIG VVGSTKRELG GSELYVRLGL KGGIAPRVNL EEEKANAEGI LEAIRRGLLK 610 620 630 640 650 660 AVHDVSKGGI AVALAEMAVL GNTGFTADLS KVPAETSNPL EVAFSESHGR YIVVFPEERL 670 680 690 700 710 EELKALFRHF AVIGRAGGSG AVFLWNGDEL LRKPITKLRE VHESFPKLLG EEE

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84386.1.
RefSeq	YP_182610.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JFL7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000007144; EBPYRP00000006925; EBPYRG00000007143.
GeneID	3235963.
GenomeReviews	Gene locus TK0197 in contig AP006878_GR.
KEGG	tko:TK0197.
NMPDR	fig|69014.3.peg.452.
Phylogenomic databases
eggNOG	arNOG04101.
GeneTree	EBGT00050000022555.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q5JFL7.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0197-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRKO

Accession

Primary (citable) accession number: Q5JFL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program