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Protein

DNA polymerase sliding clamp 1

Gene

pcn1

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.UniRule annotation

Enzyme regulationi

Inhibited by interaction with the PCNA inhibitor TIP.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA polymerase processivity factor activity Source: GO_Central
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-530-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clamp 1UniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homolog 1UniRule annotation
Short name:
PCNA 1UniRule annotation
Gene namesi
Name:pcn1UniRule annotation
Ordered Locus Names:TK0535
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492081 – 249DNA polymerase sliding clamp 1Add BLAST249

Interactioni

Subunit structurei

Homotrimer (PubMed:27141962). The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA (By similarity). Interacts with TIP (PubMed:24728986, PubMed:27141962).UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-15908613,EBI-15908613

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-59610N
STRINGi69014.TK0535

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 23Combined sources14
Beta strandi25 – 31Combined sources7
Beta strandi33 – 41Combined sources9
Beta strandi47 – 54Combined sources8
Helixi55 – 57Combined sources3
Beta strandi58 – 65Combined sources8
Beta strandi67 – 72Combined sources6
Helixi73 – 80Combined sources8
Beta strandi88 – 93Combined sources6
Beta strandi95 – 114Combined sources20
Beta strandi132 – 137Combined sources6
Helixi138 – 151Combined sources14
Beta strandi153 – 160Combined sources8
Beta strandi163 – 169Combined sources7
Beta strandi174 – 180Combined sources7
Beta strandi186 – 193Combined sources8
Beta strandi195 – 200Combined sources6
Helixi201 – 208Combined sources8
Beta strandi216 – 221Combined sources6
Beta strandi227 – 233Combined sources7
Turni234 – 236Combined sources3
Beta strandi237 – 243Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LX1X-ray2.00A1-249[»]
5DA7X-ray2.80A/D1-249[»]
5DAIX-ray2.00A1-249[»]
ProteinModelPortaliQ5JF32
SMRiQ5JF32
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5JF32

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488 Archaea
COG0592 LUCA
HOGENOMiHOG000222371
InParanoidiQ5JF32
KOiK04802
OMAiSDGFDKY
OrthoDBiPOG093Z0814

Family and domain databases

HAMAPiMF_00317 DNApol_clamp_arch, 1 hit
InterProiView protein in InterPro
IPR000730 Pr_cel_nuc_antig
IPR022649 Pr_cel_nuc_antig_C
IPR022659 Pr_cel_nuc_antig_CS
IPR022648 Pr_cel_nuc_antig_N
PANTHERiPTHR11352:SF0 PTHR11352:SF0, 1 hit
PfamiView protein in Pfam
PF02747 PCNA_C, 1 hit
PF00705 PCNA_N, 1 hit
PRINTSiPR00339 PCNACYCLIN
TIGRFAMsiTIGR00590 pcna, 1 hit
PROSITEiView protein in PROSITE
PS01251 PCNA_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JF32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFEVVFDGA KEFADLIATA SNLIDEAAFK FTEEGISMRA MDPSRVVLID
60 70 80 90 100
LNLPESIFSK YEVEEPETIG INMDQFKKIL KRGKAKDTLI LRKGDENFLE
110 120 130 140 150
ITFEGTAKRT FRLPLIDVEE LELELPELPF TAKVVLLGEV LKEGIKDASL
160 170 180 190 200
VSDAIKFIAK ENEFTMKAEG ETNEVEIRLT LEDEGLLDLE VEEETKSAYG
210 220 230 240
IRYLSDMVKG IGKADEVILR FGNEMPLQME YMIRDEGRLT FLLAPRVEE
Length:249
Mass (Da):28,239
Last modified:February 15, 2005 - v1
Checksum:iAC836C2F0E2C79CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD84724.1
RefSeqiWP_011249490.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD84724; BAD84724; TK0535
GeneIDi3234167
KEGGitko:TK0535
PATRICifig|69014.16.peg.523

Similar proteinsi

Entry informationi

Entry nameiPCNA1_THEKO
AccessioniPrimary (citable) accession number: Q5JF32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health