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Protein

S-adenosylmethionine synthase

Gene

mat

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (mat)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi139 – 1446ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-555-MONOMER.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:matUniRule annotation
Ordered Locus Names:TK0545
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405S-adenosylmethionine synthasePRO_0000150039Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi69014.TK0545.

Structurei

Secondary structure

1
405
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 125Combined sources
Helixi18 – 203Combined sources
Beta strandi21 – 299Combined sources
Helixi34 – 5724Combined sources
Beta strandi63 – 719Combined sources
Beta strandi74 – 763Combined sources
Beta strandi83 – 864Combined sources
Beta strandi88 – 10013Combined sources
Helixi107 – 12216Combined sources
Turni128 – 1303Combined sources
Beta strandi131 – 1366Combined sources
Beta strandi139 – 1435Combined sources
Helixi145 – 15410Combined sources
Beta strandi165 – 1706Combined sources
Helixi174 – 18613Combined sources
Helixi189 – 1946Combined sources
Beta strandi198 – 20912Combined sources
Beta strandi212 – 22211Combined sources
Helixi223 – 2253Combined sources
Helixi229 – 25022Combined sources
Beta strandi255 – 2595Combined sources
Turni265 – 2684Combined sources
Beta strandi272 – 2776Combined sources
Helixi278 – 2814Combined sources
Turni313 – 3153Combined sources
Helixi317 – 33519Combined sources
Beta strandi339 – 3479Combined sources
Beta strandi358 – 3669Combined sources
Helixi372 – 3743Combined sources
Helixi376 – 38813Combined sources
Turni389 – 3913Combined sources
Helixi392 – 3987Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L4QX-ray2.00A/B/C/D1-405[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01678. Archaea.
COG1812. LUCA.
HOGENOMiHOG000229842.
InParanoidiQ5JF22.
KOiK00789.
OMAiHNTDQVE.

Family and domain databases

HAMAPiMF_00136. S_AdoMet_synth2.
InterProiIPR027790. AdoMet_synthase_2_family.
IPR002795. S-AdoMet_synthetase_arc.
[Graphical view]
PfamiPF01941. AdoMet_Synthase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JF22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKVRNIVV EELVRTPVEM QKVELVERKG IGHPDSIADG IAEAVSRALS
60 70 80 90 100
REYVKRYGII LHHNTDQVEV VGGRAYPQFG GGEVIKPIYI LLSGRAVEMV
110 120 130 140 150
DREFFPVHEI ALKAAKDYLR KAVRHLDLEH HVIIDSRIGQ GSVDLVGVFN
160 170 180 190 200
KAKKNPIPLA NDTSFGVGYA PLSETEKIVL ETEKYLNSDE FKKKYPAVGE
210 220 230 240 250
DIKVMGLRKG DEIDLTIAAA IVDSEVDNPD DYMAVKEAIY EAAKGIVESH
260 270 280 290 300
TERPTNIYVN TADDPKEGIY YITVTGTSAE AGDDGSVGRG NRVNGLITPN
310 320 330 340 350
RHMSMEAAAG KNPVSHVGKI YNILSMLIAN DIAEQVEGVE EVYVRILSQI
360 370 380 390 400
GKPIDEPLVA SVQIIPKKGY SIDVLQKPAY EIADEWLANI TKIQKMILED

KVNVF
Length:405
Mass (Da):44,615
Last modified:February 15, 2005 - v1
Checksum:i13F0938F4ED2E6B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD84734.1.
RefSeqiWP_011249500.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD84734; BAD84734; TK0545.
GeneIDi3233990.
KEGGitko:TK0545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD84734.1.
RefSeqiWP_011249500.1. NC_006624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L4QX-ray2.00A/B/C/D1-405[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK0545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD84734; BAD84734; TK0545.
GeneIDi3233990.
KEGGitko:TK0545.

Phylogenomic databases

eggNOGiarCOG01678. Archaea.
COG1812. LUCA.
HOGENOMiHOG000229842.
InParanoidiQ5JF22.
KOiK00789.
OMAiHNTDQVE.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
BioCyciTKOD69014:GH72-555-MONOMER.

Family and domain databases

HAMAPiMF_00136. S_AdoMet_synth2.
InterProiIPR027790. AdoMet_synthase_2_family.
IPR002795. S-AdoMet_synthetase_arc.
[Graphical view]
PfamiPF01941. AdoMet_Synthase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiMETK_THEKO
AccessioniPrimary (citable) accession number: Q5JF22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: November 11, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.