Reviewed, UniProtKB/Swiss-Prot Q5JEZ2 (GYAR_PYRKO)

Names and origin

Protein names

Recommended name:
    Glyoxylate reductase
    EC=1.1.1.26

Gene names

Name:	gyaR
Ordered Locus Names:	TK0683

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

311400 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Glycolate + NAD+ = glyoxylate + NADH. HAMAP MF_00776
Subunit structure	Homodimer By similarity. HAMAP MF_00776
Subcellular location	Cytoplasm By similarity HAMAP MF_00776.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Glyoxylate reductase HAMAP MF_00776		PRO_0000075950
Regions
	Nucleotide binding	158 – 161	4	NADP By similarity
	Nucleotide binding	180 – 182	3	NADP By similarity
	Nucleotide binding	239 – 241	3	NADP By similarity
	Nucleotide binding	288 – 290	3	NADP By similarity
Sites
	Active site	241	1	By similarity
	Active site	270	1	By similarity
	Active site	288	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5JEZ2-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 5D836274189CC8D2	FASTA	333	37,931
	10 20 30 40 50 60 MRPKVFITRA IPENGIEMLK EHFEVEVWPE EREIPREVLL KKVRDVDALV TMLSERIDSE 70 80 90 100 110 120 VFDAAPRLRI VANYAVGYDN IDVEEATRRG IYVTNTPDVL TDATADFAWT LLLATARRLI 130 140 150 160 170 180 EADHFTRSGE WKRRGIAWHP RWFLGYDVYG KTIGIVGFGR IGQAVARRAR GFGMRILYYS 190 200 210 220 230 240 RSRKPEAEKE LGAEFRSLED LLRESDFVVL AVPLTKETQY MINEERLRLM KKTAILVNIA 250 260 270 280 290 300 RGKVVDTKAL MKALKEGWIA GAGLDVYEEE PYYNEELFSL KNVVLAPHIG SATYGAREGM 310 320 330 AELVARNLIA FKNGEVPPTL VNKEVVKVRK PGF

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD84872.1.
RefSeq	YP_183096.1.
3D structure databases
SMR	Q5JEZ2. Positions 1-333.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5JEZ2.
Genome annotation databases
GeneID	3234458.
GenomeReviews	Gene locus TK0683 in contig AP006878_GR.
KEGG	tko:TK0683.
NMPDR	fig|69014.3.peg.871.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04496.
HOGENOM	HBG731446.
OMA	MMKPTAI.
PhylomeDB	Q5JEZ2.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0683-MONOMER.
BRENDA	1.1.1.26. 192130.
Family and domain databases
HAMAP	MF_00776. GyaR. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYAR_PYRKO

Accession

Primary (citable) accession number: Q5JEZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)