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Q5JEY0 (DHYS_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable deoxyhypusine synthase

Short name=DHS
EC=2.5.1.46
Gene names
Name:dys
Ordered Locus Names:TK0671
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue By similarity. HAMAP-Rule MF_00153

Catalytic activity

[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine. HAMAP-Rule MF_00153

Cofactor

NAD By similarity. HAMAP-Rule MF_00153

Pathway

Protein modification; eIF5A hypusination. HAMAP-Rule MF_00153

Sequence similarities

Belongs to the deoxyhypusine synthase family.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   LigandNAD
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdeoxyhypusine biosynthetic process from spermidine

Inferred from electronic annotation. Source: UniProtKB-HAMAP

peptidyl-lysine modification to hypusine

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondeoxyhypusine synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Probable deoxyhypusine synthase HAMAP-Rule MF_00153
PRO_0000134506

Sites

Active site3081Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5JEY0 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 776B14C6D3BEBF36

FASTA33738,214
        10         20         30         40         50         60 
MTEPKDIVLK ESEEVEGIPI EGPWLDDVSS LEEVLDYYER IGFQATHLGK AIEIWKKVEK 

        70         80         90        100        110        120 
RRAEGKEVRV FLGYTSNIIS SGLRELVAWL VKEGKIDVIV TTAGGIEEDF IKALKPFILG 

       130        140        150        160        170        180 
DWHVNDALMR EKGINRIGNI FVPNDRYIEF EKYMIPFFER VLEIEKERGK PLTASEFIYE 

       190        200        210        220        230        240 
LGRYMDEKLG KEKERSVIYW AYKRNVPIFC PAITDGSIGD MLYFFKEERG DRELIIDIAN 

       250        260        270        280        290        300 
DIVKLNNLAV TAKETASIIL GGSLPKHAII NANLFRGGTD YAIYITTAVP WDGSLSGAPP 

       310        320        330 
SEGVSWGKIR AKADYVEIWA DATLVFPLLV WKVMKAP 

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD84860.1.
RefSeqYP_183084.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JEY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK0671.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD84860; BAD84860; TK0671.
GeneID3234536.
KEGGtko:TK0671.

Phylogenomic databases

eggNOGCOG1899.
HOGENOMHOG000228838.
KOK00809.
OMAVEIWADA.
ProtClustDBPRK03971.

Enzyme and pathway databases

BioCycTKOD69014:GH72-682-MONOMER.
UniPathwayUPA00354.

Family and domain databases

Gene3D3.40.910.10. 1 hit.
HAMAPMF_00153. DHS.
InterProIPR022899. Deoxyhypus_synthase_arc.
IPR002773. Deoxyhypusine_synthase.
[Graphical view]
PANTHERPTHR11703. PTHR11703. 1 hit.
PfamPF01916. DS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00321. dhys. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDHYS_THEKO
AccessionPrimary (citable) accession number: Q5JEY0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways