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Q5JEW5 (NADK_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:TK2124
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278NAD kinase HAMAP-Rule MF_00361
PRO_0000120708

Regions

Nucleotide binding67 – 682NAD By similarity
Nucleotide binding137 – 1382NAD By similarity
Nucleotide binding178 – 1836NAD By similarity

Sites

Active site671Proton acceptor By similarity
Binding site721NAD By similarity
Binding site1481NAD By similarity
Binding site1651NAD By similarity
Binding site1671NAD By similarity
Binding site2021NAD; via carbonyl oxygen By similarity
Binding site2371NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5JEW5 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 3F7EFF3030B7D070

FASTA27831,197
        10         20         30         40         50         60 
MKFGVVARRD KLEALKLAYR VYDFLKVSGF DVVVDEDTYR YLGEFSEDDV LPLEEFDVDI 

        70         80         90        100        110        120 
IVVIGGDGTI LRVEHKTKKE IPILGINMGT LGFLTEVEPH ETFFALSRVI EGDYHIDERI 

       130        140        150        160        170        180 
KLRTFLDGEN RVPDALNEVA VLTGIPGKII HLKYYIDGGL ADEVRADGLI ISTPTGSTGY 

       190        200        210        220        230        240 
AMSAGGPFVD PRLDVVVIAP LAPIALSSRP MVVPSSSRID VRNVAMTREV ILSVDGQFYT 

       250        260        270 
YLSPETEITI VRSPRKTKFV RFNREIYPKY TMKIKSRF 

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD86313.1.
RefSeqYP_184537.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JEW5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING69014.TK2124.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD86313; BAD86313; TK2124.
GeneID3234844.
KEGGtko:TK2124.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAPRKTKFV.

Enzyme and pathway databases

BioCycTKOD69014:GH72-2166-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_THEKO
AccessionPrimary (citable) accession number: Q5JEW5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: July 9, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families